李丰+王炜+姜开文+等

广州医科大学附属第一医院肝胆外科，广东广州 510000

[摘要] 目的 探讨大鼠肝细胞癌变过程中survivin与livin的动态表达及其二者的相互关系。 方法 随机将100只SD大鼠分为模型组与对照组各50只，模型组大鼠给予0.01%DEN自由饮用18周诱导大鼠肝癌模型，对照组大鼠常规自由饮水，于4、8、12、16、18周分别处死模型组10只，对照组10只。采用免疫组化、RT-PCR、Western bolt法检测survivin和livin的表达。 结果 免疫组化结果显示：模型组大鼠survivin、livin表达总阳性率分别为82%（41/50）、86%（43/50），survivin与livin都表达于肝癌组织及癌旁肝组织中，且二者的表达重叠，Spearman相关分析表明，二者的表达有相关性（r=0.9027，P < 0.05）。RT-PCR检测结果显示，肝癌组织中均有survivin mRNA和livin mRNA表达，随着肝癌程度的加重而增加，与对照组肝组织比较，差异有统计学意义（P < 0.05）。Western blot结果显示，survivin、livin的表达随着肝癌程度的加重而增加，对照组肝组织比较，差异有统计学意义（P < 0.05）。 结论 survivin、livin在肝癌组织中高表达，且二者的表达密切相关，为肝细胞癌的临床治疗以及预后提供理论依据。

[关键词] 大鼠；肝细胞癌；Survivin；Livin

[中图分类号] R735.7 [文献标识码] A [文章编号] 1673-7210（2014）03（b）-0027-04

Dynamic expression and correlation of survivin and livin during hepatocellular carcinogenesis in rats

LI Feng WANG Wei JIANG Kaiwen ZHANG Longxin

Department of Hepatobiliary Surgery， the First Affiliated Hospital of Guangzhou Medical University， Guangdong Province， Guangzhou 510000， China

[Abstract] Objective To determine the dynamic expression and correlation of survivin and livin during hepatocellular carcinogenesis in rats. Methods 100 SD rats were divided into model group and control group with 50 rats in each group. Rats in the model group were given 0.01%DEN for drink induced model of liver cancer， while rats in the control group were given conventional water for drink. 10 rats in model group and control group were killed at 4， 8， 12， 16， 18 weeks respectively. Survivin and livin were measured by immunohistochemistry， RT-PCR， Western bolt. Results The results of immunohistochemistry showed that the total positive rates of survivin and livin were 82% （41/50）， 86% （43/50） in the model group， survivin and livin expressed in hepatic cellular cancer tissues and para-carcinoma tissue， the expression of them was overlapping. Spearman correlation analysis showed that the correlation between them was positive （r=0.9027， P < 0.05）. The results of RT-PCR showed that survivin mRNA and livin mRNA were expressed in hepatic cellular cancer tissues， increased with the liver cancer degree， compared with the control group， the difference was statistically significant （P < 0.05） .The results of Western blot showed that the expression of survivin and livin increased with the increase of hepatocellular carcinoma of liver tissue. There was statistically significant difference compared with the control group （P < 0.05）. Conclusion Survivin and livin are highly expressed in hepatic celluar Cancer tissues， and the expression of them are closely related. The results may provide a theoretical basis for the clinical treatment and prognosis of hepatocellular carcinoma.

[Key words] Rat； Hepatocellular carcinoma； Survivin； Livin

肝细胞癌（HCC）是临床中常见的肿瘤，大部分进展期的病例缺乏有效的临床治疗，日益受到重视[1]。无限的细胞增殖和损伤凋亡的不平衡是HCC重要的特征[2]。IAP家族是重要的凋亡抑制因子。目前已发现8种IAPs，作为家族的新成员，survivin被认为在肿瘤的发生、发展中扮演重要角色；livin的研究则刚刚开始，其在肝肿瘤组织中的表达研究更不完善，在我国鲜有livin在肝癌组织中的研究报道。本文用免疫组化、Western bolt、RT-PCR法检测抗凋亡蛋白livin、survivin蛋白和mRNA在肝癌组织中的表达，探讨二者之间相互关系，从而为临床诊断和肝癌的治疗提供理论依据。……