刘以琨,谭艾娟,吕世明,方英

摘要：为了解贵阳市市售猪肉中食源性致病菌的污染情况，采集贵阳市市售猪肉样品60份，按照相关国家标准分离鉴定样品中的沙门氏菌、志贺氏菌、单核细胞增生李斯特菌、金黄色葡萄球菌、肠出血性大肠杆菌。结果表明：样品中食源性致病菌总检出率为13.33%，其中沙门氏菌检出率为6.67%，金黄色葡萄球菌检出率为3.33%，志贺氏菌检出率为1.67%，单核细胞增生李斯特菌检出率为1.67%，肠出血性大肠杆菌未检出。说明贵阳市市售猪肉中沙门氏菌的污染率最高，金黄色葡萄球菌、志贺氏菌和单核细胞增生李斯特菌也存在一定污染情况。

关键词：食源性致病菌；猪肉；检测；食品安全

中图分类号：TS207.4文献标识码：ADOI编码：10.3969/j.issn.1006－6500.2012.02.021

Isolation and Identification of the Food-borne Pathogen in Retail Pork of Guiyang City

LIU Yi-kun1，TAN Ai-juan1， LV Shi-ming2，FANG Ying3

(1. College of Life Science, Guizhou University, Guiyang, Guizhou 550025,China;2. College of Animal Science, Guizhou University, Guiyang, Guizhou 550025,China;3. Agriculture Committee of Guiyang City, Guiyang，Guizhou 550081,China）

Ａｂｓｔｒａｃｔ: To investigate the pollution of five food-borne pathogenic bacteria in retail pork of Guiyang city, the sixty pork samples were collected from supermarket and open fairs. The five food-borne pathogenic bacteria in samples were isolated and identified according to the national standard. The result showed the total detection rate of five food-borne pathogenic bacteria was 13.33%, among which the Salmonella, Staphylococcus aureus, Shigella and Listeria monocytogene were 6.67%, 3.33%, 1.67% and 1.67% respectively. Escherichia coli O157∶H7 was not found. The pollution rate of Salmonella in retail pork in Guiyang city was highest among five food-borne pathogenic bacteria, and Staphylococcus aureus, Shigella and Listeria monocytogene were still detected.

Key words: food-borne pathogens; pork; detection; food safety

食品安全问题关乎人类健康，对国民经济和民生问题造成严重的冲击，影响整个社会的稳定和经济发展[1-2]。目前我国政府高度重视食品安全问题，正在采取一系列措施加强食品安全的监管，尤其是肉食品的安全监管[3-4]。猪肉是我国的主要肉食品，关注猪肉的安全意义重大，笔者对贵阳市市售猪肉中食源性致病菌进行检测，了解贵阳市市售猪肉中食源性致病菌的污染情况，为有效地预防和控制贵阳市猪肉引发的食源性疾病的发生提供依据。

1材料和方法

1.1材料

60份猪肉样品分别采自贵阳市南明区、云岩区、乌当区、花溪区、白云区、小河区、息烽县、开阳县、修文县、清镇市的农贸市场。

细菌分离培养基为改良麦康凯培养基。缓……