王业秋,陈巧云,张宁,井丽巍,祁永华

[摘要]目的：探讨玉竹水提液对中波紫外线（UVB）诱导人皮肤角质形成细胞损伤的保护作用及其可能机制。方法：用64mJ·cm-2的UVB照射人皮肤角质形成细胞建立光老化细胞模型，以不同浓度玉竹提取物处理光老化细胞，羟胺法检测细胞超氧化物歧化酶（SOD）活性，比色法检测谷胱甘肽过氧化物酶（GSH-Px）活性，TBA法检测丙二醛（MDA）含量，酶联免疫法检测肿瘤坏死因子α（TNF-α）、白介素6（IL-6）分泌量。结果：UVB照射角质形成细胞后，SOD、GSH-Px活性降低，MDA含量升高，TNF-α、IL-6分泌量增加（P<0.01），玉竹水提液中（100μg·ml-1）、高（200μg·ml-1）剂量组能显著提高SOD、GSH-Px活性，降低MDA含量，减少TNF-α、IL-6分泌（P<0.05或P<0.01）。结论：玉竹水提液可在一定程度上减轻UVB对角质形成细胞的损伤，其机制可能与其抑制氧化损伤，增强细胞抗氧化能力，减少炎症细胞因子分泌有关。

[关键词]玉竹；UVB；HaCaT细胞；保护作用

[中图分类号]Q813.1[文献标识码]A[文章编号]1008-6455（2012）04-0599-03

Protective effects of yuzhu on hacat cells damaged by ultraviolet B

WANG Ye-qiu,CHEN Qiao-yun,ZHANG Ning,JING Li-wei,QI Yong-hua

(Institute of Traditional Chinese Medicine &Cosmetology,Faculty of Jiamusi,Heilongjiang University of Chinese Medicine, Jiamusi 154007,Helongjiang,China)

Abstract:ObjectiveTo investigate the protective effect of water-extract solution of Yuzhu on HaCaT cells injured by irradiation of ultraviolet B and its possible mechanisms.MethodsCell photoaging model was established by irradiating HaCaT cells with UVB(64mJ·cm-2). HaCaT cells were treated with different concentration water-extract solution of Yuzhu. The activity of superoxide dismutase (SOD) and glutathione peroxidase(GSH-Px),the content of malondialdehyde(MDA) were detected by biochemical assay, the secretion levels of TNF-α and IL-6 were detected by enzyme-linked immunosorbent assay (ELISA).ResultsThe proliferation activity of HaCaT cells descended with the UVB irradiation, the activities of SOD and GSH-Px decreased, when the content of MDA, TNF-α, IL-6, were ascensive(P<0.01). But under the intervention at middle concentration(100μg·ml-1) and high concentration(200μg·ml-1) of water-extract solution of Yuzhu, the above changes induced by UVB irradiation were inhibited(P<0.05 or P<0.01).ConclusionThe water-extract solution of Yuzhu might inhibit the proliferation decreasing which induced by UVB. And its mechanism may have relationship with the ability of strengthening antioxidation, decreasing oxygen radicals and decreasing the secretions of proinflammatory cytokines.

Key words:Yuzhu;UVB;HaCaT cells;protective effects

随着环境污染的日益严重，到达地球表面的UV日益增多，UV辐射对健康的危害已成为全球关注的重大环境与健康问题之一，有关UV辐射的机制和防治研究是目前的研究热点。中药以其独特的优势，在抗皮肤光损伤领域的研究工作得到广泛开展，玉竹为百合科植物玉竹Polygonatum odoratum(Mill.)Druce的干燥根茎，现代药理学研究表明玉竹具有抗氧化、清除自由基、提高免疫力、抗肿瘤等多种生物活性[1-3]。笔者以体外培养的人皮肤角质形成细胞（HaCaT）为研究对象，在体外成功建立UVB诱导HaCaT细胞光损伤模型，观察玉竹水提液对UVB损伤HaCaT细胞的保护作用。

1材料和仪器

1.1 实验材料：人皮肤角质形成细胞HaCaT（武汉大学细胞中心），MEM培养基（Gibco公司），胎牛血清、胰蛋白酶、双抗（Billab公司），MTT（Sigma公司），超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、丙二醛（MDA）测定试剂盒（南京建成生物工程公司），TNF-α、IL-6（研域(上海)化学试剂有限公司），中药玉竹（北京同仁堂制药集团哈尔滨分店，经本校陈效忠副教授鉴定为正品）。……