王晓燕 郑岩 孟宝玺 夏炜 马显杰 王志军

[摘要]目的：探讨经耳源静脉移植入体内的骨髓间充质干细胞（Bone marrow-derived mesenchymal stem cells ，BM-MSCs）参与皮肤扩张的情况。方法：以小型家猪为研究对象，分离培养并鉴定猪BM-MSCs。制作猪皮肤扩张模型，8头猪随机分为两组，每组4头，实验组（A组）：扩张后耳源静脉注入DiI标记的猪BM-MSCs；对照组（B组）：扩张后耳源静脉注入等量PBS，分别于扩张后7、14、28天测量两组扩张面积，冰冻切片观察标记细胞在扩张皮肤中的比例和分布情况，Real-time PCR检测基质细胞衍生因子-1（SDF-1）表达情况。结果：流式细胞仪鉴定细胞CD29、CD90表达阳性，CD34、CD45表达阴性，细胞标记率＞99%。扩张后第14天、28天实验组扩张面积较对照组明显增加（P＜0.05），冰冻切片显示，14天时实验组扩张皮肤可见DiI标记细胞聚集。PCR示SDF-1表达量实验组是对照组的5.8倍。结论：经静脉移植的BM-MSCs可迁移至扩张局部，并能有效促进皮肤扩张。

[关键词]骨髓间充质干细胞；静脉移植；皮肤扩张

[中图分类号]R622Q813.1[文献标识码]A [文章编号]1008-6455（2012）04-0596-03

Study on the feasibility of BM-MSCs transplanted through systemic vein to improve the skin expansion

WANG Xiao-yan1,ZHENG Yan1,MENG Bao-xi2,XIA Wei1,MA Xian-jie1,WANG Zhi-jun3

（1Institutes of Plastic Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an710032, Shaanxi, China；2.Department of Plastic Surgery,The Fifst People's Hospital, Zhengzhou；3.Department of Plastic Surgery, Affiliated Xinhua Hospital of Dalian Medical College）

Abstract:ObjectiveTo investigate the feasibility of transplantation of mesenchymal stem cell from ear vein into the skin and soft tissue expansion model.Methods MSCs were isolated from porket's bone marrow and cultured in vitro. Eight pigs were randomly divided into two groups: The group A was injected with MSCs which dyed with CM-DiI from ear vein; the group B was injected with PBS from ear vein as the control group. The expansion area was measured on day7,14,28 after operation and the proportion and distribution of labeled cells were detected by frozen section . Real-time quantitative PCR was used to quantify the expression of chemotactic growth factor-1(SDF-1).Results The MSCs cultured expressed CD90 and CD29 highly but didn't express CD34 or CD45. The mark rate>99%. On day 14 and 28, the area of experimental group was increased significantly,compared to the control group (P＜0.05). The result of frozen section show that the DiI-labled cells were gathered together in the experimental group on day 14 after expansion. Real-time quantitative PCR show that the target gene expression of SDF-1 was 5.8-fold higher than the control group. Conclusion BM-MSCs transplantated from ear vein can migrate to the local of expansion and promote the skin expansion.

Key words:bone marrow mesenchymal stem cells (BM-MSCs); vein transplantation; skin expansion

皮肤扩张术由于其良好的修复效果，成为整形外科最常用的修复手段之一。但皮肤扩张术存在治疗周期长、术后皮瓣回缩的缺点，如何在短时间扩张出更多新生的皮肤成为有待解决的问题。骨髓间充质干细胞由于其多项分化功能、活性强、易分离培养等优点成为创伤修复领域的热点[1-2]，而皮肤扩张术本质上也是创伤修复的过程，因此本实验经静脉移植染色标记后的骨髓间充干细胞于扩张器模型，观察其在扩张皮肤内的分布和比例，拟为间充质干细胞移植促进扩张皮肤新生探寻一种无创性的移植方案。