王莲 魏玲 付莉

[摘要] 目的：应用Fluoro-Jade C（FJC）染色方法在小鼠匹罗卡品癫痫模型中探测纹状体结构中神经元的变性情况，以了解纹状体结构在慢性颞叶癫痫发生中的病理神经基础。方法：雄性昆明小鼠10只（对照组5只，模型组5只）。在癫痫持续状态后3 d处死模型组小鼠。在纹状体水平切制冠状切片，行FJC染色，在荧光显微镜下观察FJC阳性细胞的形态和在纹状体（尾状壳核）中的整体分布情况。结果：在模型组，FJC染色的脑切片上清楚显示FJC阳性变性细胞呈亮黄绿色，呈神经元形态，胞体和突起均清晰显示。在纹状体（尾状壳核）内背侧1/3和尾侧部分出现许多FJC阳性细胞。结论：本研究运用FJC染色技术在小鼠匹罗卡品癫痫模型中显示纹状体中发生了神经元变性，提示纹状体可能在慢性颞叶癫痫的发生机制中起重要作用。

[关键词] Fluoro-Jade C；癫痫；匹罗卡品；神经变性

[中图分类号] R742.1 [文献标识码]A[文章编号]1673-7210（2011）11（c）-021-03

Fluoro-Jade C staining in the assessment of neurodegeneration in the striatum of Pilocarpine-treated mice

WANG Lian, WEI Ling, FU Li

Local Cadres Ward, Kunming General Hospital of Chengdu Military Region, Yunnan Province, Kunming 650032, China

[Abstract] Objective: To detect degenerative neurons by Fluoro-Jade C (FJC) that may occurred in the striatum of pilocarpine-treated mice and to understand the pathologically neural basis of striatum in chronic epilepsy. Methods: Male adult mice (Kunming stain, n=10) were divided into Pilocarpine-treated group (n=5) and control group (n=5). Pilocarpine-treated mice were sacrificed at 3 d after status epilepticus. Brain coronal sections in the striatum levels were subject to FJC staining. Finally, FJC-stained brain and overall distribution of the Fluoro-Jade C positive cells sections were examined under an epifluorescence microscope to identify the shape in the striatum (caudate putamen). Results: The FJC-positive staining cells showed a bright green color in the somas and fine processes with neuronal profiles. Numerous degenerating cells could be seen in the medial dorsal one-third and caudal part of the striatum (caudate putamen). Conclusion: Massive neurodegeneration are demonstrated by FJC staining in the striatum of Pilocarpine-treated mice, suggesting that the striatum may play a role in recurrent seizure mechanism of chronic epilepsy.

[Key words] Fluoro-Jade C; Epilepsy; Pilocarpine; Neurodegeneration

颞叶癫痫是人类癫痫的常见形式，治疗难度大，发病机制复杂，目前仍有许多问题有待解决。中枢神经系统中许多关键性区域涉及颞叶癫痫的发作，如基底节在癫痫活动的启动和传播方面起重要作用[1]。在本研究中笔者应用新近发展的一类阴离子荧光配体组化染料Fluoro-Jade C（FJC，特异性标记中枢神经系统中正在变性的神经元[2-4]）和小鼠匹罗卡品颞叶癫痫模型研究了纹状体神经元的变性情况，深入了解慢性颞叶癫痫中癫痫反复发作的神经基础。

1 材料与方法

1.1 材料

实验动物为雄性昆明小鼠10只，鼠龄4～5周，体重20～25 g。……