第1期

·研究报告·

铜离子胁迫对近江牡蛎软体组织及贝壳重金属含量的影响

卓 文，陈 新，刘秋妤，唐 敏(1)

益生菌和复合营养剂对鱼虾混养池塘水质及细菌群落结构的影响

陈锦豪，郑锦滨，毛 勇，苏永全，王 军(6)

三亚国家级珊瑚礁自然保护区珊瑚礁资源的多样性

吴川良, 李长青, 张文勇, 耿涛年, 李 颖， 刘亚星, 田晶晶(14)

珊瑚的室内循环海水生态养殖模式的构建

黄 敏，王荣霞，王永波，蒲利云，唐贤明(22)

16种植物源活性成分对2种芒果采后病害的防治

苏子寒，高兆银，胡美姣，李 敏，张正科(28)

非洲楝拟茎点霉叶斑病病原菌鉴定及其生物学特性测定

林 宇，李增平，吴如慧，张 宇(34)

1株耐多种重金属菌株的分离鉴定

刘士尧，刘珮熠，徐羽丰，龙云飘，牛晓磊，李春霞，陈银华，陶 均(41)

插皮接技术在海南油茶低产林高接换冠的应用

徐家洪，曾 晴，张毛宁，胡新文，吴友根，王 健，陈金辉，，赖杭桂(47)

橡胶树HbHMGS1启动子对光照、干旱及热击处理的表达响应

巩笑笑，闫冰玉，谭玉荣，王 鹏，李双江，王 艺，周璐瑶，刘进平(52)

海南冬季诱导火龙果开花的补光条件

熊 睿, 徐 敏, 刘成立, 林家年，程 玉，韦双双, 汤 华(60)

钝药野木瓜果实全营养素分析测定

刘天虎，刘积光(66)

2种调查方法对兴隆植物园游人分布及行为分析

刘维维，康梓杭，黄亚琼，邓 晶，吴庆书(70)

海口市街巷系统开放空间的有效性评价

陈 隆，林世平(76)

·技术方法·

反相高效液相色谱法对小鼠血清中基因重组人促肾上腺皮质激素前体及其代谢物的测定

武新丽，韦双双，裴业春，赵景锋，王大勇(83)

β-葡萄糖苷酶抗体的制备及比较分析

余厚美，张振文(89)

濒危兰科植物DNA的提取方法

潘英文，张 凌，王安石，李佳潼，谢添伟，陈施明，刘福秀，林明光(94)

第2期

·研究报告·

α-芋螺毒素TxID对肺癌的细胞毒性

钱 江，孙志华，刘益巧，谢品希，长孙东亭，罗素兰(99)

水稻OsNHX5基因的亚细胞定位及表达分析

王丹阳，范亚飞，周 扬，罗明华，江行玉，罗越华，夏幽泉(106)

木薯MeNRT2.5基因的克隆及表达分析

任 宁，陈秀珍，夏幽泉，白雪杨，江行玉，周 扬 (111)

木薯MKK家族基因的鉴定及表达分析

侯鹏宇，于心怡，肖晓蓉，郑琳琳，陈银华(119)

香蕉枯萎镰刀菌4号生理小种mon1基因敲除转化子的表型分析及致病力测定

李 恒，畅文军，陈汉清，乔 帆，曾会才(127)

溴氰虫酰胺对羊角月牙藻的急性毒性效应

徐成滨，王宇心，孔伟浩，张晓凯，刘诗颖，范咏梅(135)

氟硅唑对丰年虫急性毒性及氧化应激的影响

张晓凯，刘剑南，徐成滨，张业扬，孙明凯，范咏梅(140)

槌果藤粗提物的杀虫活性

章 杰，吴接呈，骆焱平，王兰英(145)

蚯蚓处理对猪粪重金属富集的影响

黄 炜，刁晓平，李森楠，龚 莹(151)

海南龙血树人工血竭与天然血竭的抗炎镇痛活性比较

王 辉，杨 宁，吴晓鹏，戴好富，梅文莉(159)

海口羊山湿地野生维管束植物资源的调查

尹菡怿，申益春，琚青青，周 鹏(165)

老挝丰沙里省小粒咖啡园杂草的生物多样性分析

胡发广，毕晓菲，黄家雄，李亚男，严 炜，吕玉兰(172)

·技术方法·

CHRNA7基因荧光定量PCR检测方法的建立

刘益巧，孙志华，钱 江，长孙东亭，罗素兰(178)

火龙果根际土壤微生物2种DNA提取方法的比较

陈 弟，李可增，吴 琼，何应对 ，殷晓敏(184)

白木香不同结香方法所得沉香的精油成分分析

陈品品，陈日忠，吴辉煌，林文忠，马晓娟，刘 靓，庄卫东(190)

超高效液相色谱-串联质谱法检测东寨港海水中磺胺类药物残留

张腾云，徐 文，张鸣姗，赵洪伟(197)

海南菲牛蛭抗凝血酶成分的提取与SDS-PAGE电泳测定

何梦雪，李泽友(204)

·文献综述·

硫化氢在炎症和自噬等病理中作用与机制的研究进展

周 骊，林昌俊，周 鹏(208)

第3期

·研究报告·

8种生草水浸提物对狗牙根和鬼针草的化感作用

袁 洋，张苗苗，窦孟兰，朱朝华(215)

单叶蔓荆子提取物的抑菌活性

沈恬安，杨德友，骆焱平，王兰英(222)

31种瑶药提取物抑菌活性的筛选

杨若颖，李 婵，叶明亮，张丹阳，冯俊涛，闫 合(226)

刺芫荽叶乙醇提取物的抗氧化活性

1.2.4 按照医嘱服用药物对于患有慢性病的老年患者,社区护理人员应当定期上门对其进行血糖、血压的监测,结合患者的实际情况实施心理护理和生活护理,将疾病的相关知识向患者耐心讲解,药物的服用剂量、注意事项和用法向患者详细说明,将遵照医嘱用药的重要性向患者不断强调,药物的服用量不能随意增加或减少[4]。

张建春，吴接呈，张筑宏，王兰英，骆焱平(231)

雾滴粒径对川楝素在3种作物上沉积量的影响

李 海，马志卿，张 兴，周一万(235)

甲基化植物油对玉米田除草剂的增效作用

施秀飞，刘显良，李俊凯，杜晓英(240)

3种拟除虫菊酯农药在海南土壤中的降解特性

郑 钊，许佳彬，王 威，邹丽君，梁晓宇，王 萌，张 宇(244)

川楝素乳油在3种作物上的最佳喷雾条件

周一万，吴丽媛，张 兴，冯俊涛(250)

放线菌KN37菌株代谢物对番茄灰霉病的防治

邵胜楠，张安琪，巴尔那·库马尔，张国强(259)

5种植物源农药对假眼小绿叶蝉的田间药效

田向荣，李彦涛，王智辉，林 军，高小宝，吴 华，何 军(265)

60%霜霉·精甲霜水剂防治葡萄霜霉病

曹雅慧，卢海博，朱修昊，师占海，王道瑛，焦英虎，张红杰(269)

橡胶树枯萎病病原菌室内毒力测定

彭 昀，肖宇明，刘衍超，李增平，王 萌，梁晓宇，张 宇(273)

嘧啶肟草醚类化合物对稗草的室内活性测定

向兰香，张 波，李俊凯，覃兆海，杜晓英(277)

蔡永凤，肖彩云，吴 帅，郑茂彬，李 明，李荣玉(281)

稻田常用农药对螟黄赤眼蜂的影响

张唯伟,董 怡，张传清，朱国念，刘亚慧(286)

果园多种处理模式对杂草的影响

张苗苗，窦孟兰，敖 苏，朱朝华(291)

·文献综述·

内生真菌印度梨形孢提高植物抗逆性的研究进展

韩 飞，李俊凯(301)

第4期

·研究报告·

烟碱型乙酰胆碱受体β4亚基点突变体的构建

张 璐，朱晓鹏，长孙东亭，罗素兰(307)

我国蔗区甘蔗宿根矮化病发生情况的分子检测

沈林波，吴楠楠，冯小艳，王文治，熊国如，赵婷婷，张树珍(314)

濒危兰科植物DNA条形码鉴定体系的建立与优化

潘英文，张 凌，王安石，李佳潼，谢添伟，陈施明，刘福秀，林明光 (319)

增强UV-B辐射对芒果叶片光合组织结构的损伤

岳 堃，王 红，郭钰柬，周开兵(324)

栽培方式对山栏稻光合作用和产量的影响

柯 智，黄孟雨，刘志超，何美丹，郭 婷，曾丹琦，王子牛，王玥琦，袁潜华(331)

7 个甘薯品种自然富硒能力的比较

黄 婷，万 玲， 陈艳丽 ，司成成，曾丽萍(338)

50味中药及其复方与抗生素对河流弧菌的体外抑菌作用

徐晓津，李秀华，马一帆，何 俊，李慧耀，祁 欣，张蕉南，王云才，赵玲敏，鄢庆枇(343)

联合施用微生物菌剂和氨基酸水溶肥对哈密瓜土壤性质及细菌群落结构的影响

齐 钊，张曼丽，闫 臻，徐 敏，李相煌，马 叶，熊 睿，柳晓磊，汤 华(352)

茶皂素对沙门氏菌的抑菌机理及对鸡胸肉的保鲜效果

何荣荣，谭运寿，张美虹，李正香，钟秋平(360)

绿色木霉对花生黄曲霉毒素污染的防治

徐杨玉，刘付香，洪彦彬，陈小平，李海芬，温世杰，李杏瑜， 周桂元，梁炫强(367)

大尖囊蝴蝶兰内生真菌和细菌的分离与鉴定

陈耀丽，俞龙春，钱 悦，宋希强，张 哲(372)

基于大数据挖掘的橡胶园耕地地力评价

彭金莲，唐朝胜，蒋菊生，赵春梅(380)

尖峰岭热带山地雨林雨季土壤的酶活性特征及其影响因子

许 全，杨 怀，杨 秋，蒋亚敏，张书齐，刘文杰(387)

海南铜鼓岭热带滨海森林树干地衣真菌群落分析

高博钰，杨小波，李英英，李东海，张 丽，宋佳昱(396)

海南白石岭野生植物的调查及其园林价值

宋佳昱，杨小波，李东海，张 丽，高博钰(406)

城市街道开放空间活力与空间构成要素的相关性评价——以海口市海甸岛为例

段 磊，林世平，陈秋池，马山水，张 杰(417)

基于日照需求的热带城市行道树的配置——以海口市为例

汪 丽，康梓杭，吴庆书(424)

基于空间句法的海口骑楼历史文化街区更新策略

马 昊，段 磊，付 晖，任萧雨，许先升(432)

Vol.10 2019

ContentsofMainArticles

NO.1

·Researchreports·

Changes of Heavy Metals in the Soft Tissue and Shell ofCrassostrearivularisunder the Stress of Cu2+

ZHUOWen,CHENXin,LIUQiuyu,TANGMin(1)

Effects of Probiotics and Compound Nutrients on Water Quality and Community Structure of Bacteria in

Fish-shrimp Farming Pond

CHENJinhao,ZHENGJinbin,MAOYong,SUYongquan,WANGJun(6)

Diversity of Corals in Sanya Coral Reef National Nature Reserve

WUChuanliang,LIChangqing,ZHANGWenyong,GENGTaonian,LIYing,LIUYaxing,TIANJingjing(14)

Construction of Coral Recycling Aquarium Model and Analysis of Main Environmental Factors Affecting the Coral Growth

HUANGMin,WANGRongxia,WANGYongbo,PULiyun,TANGXianming(22)

Effects of Plant-based Active Ingredients on Postharvest Diseases of Mango Fruit

SUZihan,GAOZhaoyin,HUMeijiao,LIMin,ZHANGZhengke(28)

Identification and Biological Characteristics of the Pathogen CausingPhomopsisleaf spot inKhayasenegalensis

LINYu,LIZengping,WURuhui,ZHANGYu(34)

Isolation and Identification of a Multiple Resistant Bacterium to Heavy Metals

NIUShiyao,LIUPeiyi,XUYufeng,LONGYunpiao,NIUXiaolei,LIChunxia,CHENYinhua,TAOJun(41)

Application of Bark Grafting in the Topworking of Low Yielding Camellia Oleifera Plantations in Hainan

XUJiahong,ZENGQing,ZHANGMaoning,HUXinweng,WUYougen,WANGJian,CHENJinhui,LAIHanggui(47)

Expressional Responses of theHbHMGS1Promoter to Light, Drought and Heat-shock Treatments

GONGXiaoxiao,YANBingyu,TANYurong,WANGPeng,LIShuangjiang,WANGYi,ZHOULuyao,LIUJinping(52)

Supplementary Illumination for Pitaya Flower Induction in Winter in Hainan

XIONGRui,XUMin,LIUChengli,LINJianian,CHENGYu,WEIShuangshuang,TANGHua(60)

Analysis and Determination of Nutrients inStauntonialeucanthaFruit

LIUTianhu,LIUJiguang(66)

Analysis of Distribution and Behavior of Tourists in Xinglong Botanical Garden by Two Survey Methods

LIUWeiwei,KANGZihang,HUANGYaqiong,DENGJing,WUQingshu(70)

Effective Evaluation of Open Space of Urban Street System

CHENLong，LINShiping(76)

·Literaturereviews·

Determination of Recombinant Human Adrenocorticotropic Hormone Precursor Protein and Its Metabolite

in Mouse Serum by RP-HPLC

WUXinli,WEIShuangshuang,PEIYechun,ZHAOJingfeng,WANGDayong(83)

Preparation and Comparative Analysis ofβ-glucosidase Antibodies

YUHoumei,ZHANGZhenwen(89)

Extraction of Genome DNA from Endangered Orchid Plants

PANYingwen,ZHANGLing,WANGAnshi,LIJiatong,XIETianwei,

CHENShiming,LIUFuxiu,LINMingguang(94)

NO.2

·Researchreports·

Cytotoxicity ofα-conotoxin TxID in Lung Cancer

QIANJiang,SUNZhihua,LIUYiqiao,XIEPinxi，ZHANGSUNDongting,LUOSulan(99)

Subcellular Localization and Expression ofOsNHX5in Rice

WANGDanyang,FANYafei,ZHOUYang,LUOMinghua,JIANGXingyu,LUOYuehua,XIAYouquan(106)

Cloning and Expression Analysis ofMeNRT2.5Gene inCassava

RENNing,CHENXiuzhen,XIAYouquan,BAIXueyang,JIANGXingyu,ZHOUYang(111)

Identification and Expression Analysis of MKK Genes inCassava

HOUPengyu,YUXinyi,XIAOXiaorong,ZHENGLinlin,CHENYinhua(119)

Functional Analysis of themon1Gene inFusariumoxysporumf. sp.cubenseRace 4

LIHeng,CHANGWenjun,CHENHanqing,QIAOFan,ZENGHuicai(127)

Acute Toxicity of Cyantraniliprole toSelenasidiumcapricornutum

XUChengbin,WANGYuxin,KONGWeihao,ZHANGXiaokai,LIUShiyin,FANYongmei(135)

The Acute Toxicity of Flusilazole to and Induced Oxidative Stresses ofChirocephalus

ZHANGXiaokai,LIUJiannan,XUChengbinZHANGYeyang,SUNMingkai,FANYongmei(140)

Insecticidal Activity of Crude Extracts fromCapparisspinosaL.

ZHANGJie,WUJiecheng,LUOYanping,WANGLanying(145)

Effect of Earthworms on Enrichment of Heavy Metals in Pig Manure

HUANGWei,DIAOXiaoping,LISennan,GONGYing(151)

Comparison of Analgesic and Anti-inflammatory Activities Between Natural and Artificially Induced Dragon’s

Blood ofDracaenacambodiana

WANGHui,YANGNing,WUXiaopeng,DAIHaofu,MEIWenli(159)

Vascular Plant Flora of Wetland in Yangshan of Haikou City

YINHanyi,SHENYichun,JUQingqing,ZHOUPeng(165)

Analysis of Species Diversity of Weed Communities in the Arabica Coffee Plantations in Phngsali Province, Laos

HUFaguang,BIXiaofei,HUANGJiaxiong,LIYanan,YANWei,LVYulan(172)

·Technicalmethods·

Establishment of Real-time PCR for Detection ofCHRNA7Gene

LIUYiqiao,SUNZhihua,QIANJiang,ZHANGSUNDongting,LUOSulan(178)

Comparison of Two Extraction Methods in Extraction of Total DNA from Soil Microbes in the Rhizosphere

of Pitaya

CHENDi,LIKezeng,WUQiong,HEYindui,YINXiaomin(184)

Analysis of Components of Essential Oil of Agarwood fromAquilariasinensisInduced by Different Methods

CHENPinpin,CHENRizhong,WUHuihuang,LINWenzhong,

MAXiaojuan,LULiang,ZHUANGWeidong(190)

Analysis of Sulfonamides Residues in Seawater of Dongzhai Harbor, Hainan Based on Ultra Performance

Liquid Chromatography-tandem Mass Spectrometry

ZHANGTengyun,XUWen,ZHANGMingshan,ZHAOHongwei(197)

Extraction and SDS-PAGE Electrophoresis of Anticoagulant Components fromPoecilobdellamanillensis

Lesson in Hainan

HEMengxue,LIZeyou(204)

·Literaturereview·

Research Progress on the Role and Mechanism of Hydrogen Sulfide in Inflammation and Autophagy

ZHOULi,LINChang-jun,ZHOUPeng(208)

NO.3

·Researchreports·

Allelopathic Effects of Extracts of 8 Cover Crops onCynodondactylon(L.) Pers. andBidenspilosaL.

YUANYang,ZHANGMiaomiao,DOUMenglan,ZHUChaohua(215)

Antifungal Activity of the Extract fromVitextrifolia

SHENTian′an,YANGDeyou,LUOYanping,WANGLanying(222)

Screening of Antibacterial Activities of Extracts of 31 Species of Yao Medicinal Plants

YangRuoying,LiChan,YeMingliang,ZhangDanyang,YanHe(226)

Antioxidant Activity of Ethanol Extract fromEryngiumfoetidumleaves

ZHANGJianchun,WUJiecheng,ZHANGZhuhong,WANGLanying,LUOYanping(231)

Effects of Droplet Size on Deposition of Toosendanin on Three Crops

LIHai,MAZhiqing,ZHANGXing,ZHOUYiwan(235)

Synergistic Effect of Methylated Vegetable oil on Weedicides in Corn Fields(Brief)

SHIXiufei,LIUXianliang,LIJunkai,DUXiaoying(240)

Degradation Characteristics of Three Different Pyrethroid Pesticides in Soil in Hainan

ZHENGZhao,XUJiabin,WANGWei,ZOULijun,WANGMeng,ZHANGYu(244)

Optimum Spray Condition of Toosendanin EC for Three Crops

ZHOUYiwan,WULiyuan,ZHANGXing,FENGJuntao(250)

The Control Effect of Actinomycete Strain KN37 against Tomato Gray Mold

SHAOShengnan,ZHANGAnqi,BAERNAKumaer,ZHANGGuoqiang(259)

Field Efficiency of Five Botanical Pesticides againstEmpoascavitisGothe

TIANXiangrong,LIYantao,WANGZhihui,LinJun,GAOXiaobao,WUHua,HEJun(265)

Control of Grape Downy Mildew by 60% Mixture of Frostweed and Metalaxil (Aqueous Solution)

CAOYahui,LUHaibo,ZHUXiuhao,SHIZhanhai,WANGDaoying,JIAOYinghu,ZHANGHongjie(269)

Screening of Fungicides in Laboratory Against Pathogens Causing Fusarium wilt in Rubber Trees

PENGYun,XIAOYuming,LIUYanchao,LIZengping,WANGMeng,LIANGXiaoyu,ZHANGYu(273)

Determination of Herbicidal Activity of Pyribenzoxim Compounds againstEchinochloacrusgalli(L.)(Brief)

Beauv. in Greenhouse

XIANGLanxiang,ZHANGBo,LIJunkai,QINZhaohai,DUXiaoying(277)

Synergistic Effect of Sulfoxaflor and Indoxacarb on Brown Planthopper,Nilaparvata Lugens (Stål)

CAIYongfeng,XIAOCaiyun,WUShuai,ZHENGMaobin,LIMing,LIRongyu(281)

Effects of Common Insecticides onTrichogrammachilonisin Paddy Fields

ZHANGWeiwei,DONGYi,ZHANGChuanqing,ZHUGuoxian,LIUYahui(286)

Effects of Different Mulching Patterns on Weeds in Orchards

ZHANGMiaomiao,DOUMenglan,AOSu,ZHUChaohua(291)

·Literaturereview·

Research Advances in Endophytic Fungus Piriformospora Indica to Improve Plant Stress Resistance

HANFei,LIJunkai(301)

NO.4

·Researchreports·

Construction of Nicotinic Acethlcholine Receptorβ4 Subunit Mutant

ZHANGLu,ZHUXiaopeng,ZHANGSUNDongting,LUOSulan(307)

Molecular Detection of Sugarcane Ratoon Stunting Disease in Sugarcane Producing Areas of China

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Establishment and Optimization of DNA Barcoding in Endangered Orchid Plants

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Damages to the Photosynthetic Tissue Structure of Mango Leaves by Enhanced UV-B Radiation

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Effects of Cultivation Patterns on Photosynthesis, and Yield and Its Components of Shanlan Upland Rice

KEZhi,HUNAGMengyu,LIUZhichao,HEMeidan,GUOTing,

ZENGDanqi,WANGZiniu,WANGYueqi,YUANQianhua(331)

Natural Selenium-enriching Ability of 7 Sweet Potato Varieties

HUANGTing，WANLing，CHENYanli,SIChengcheng，ZENGLiping(338)

InVitroBacteriostasis of 50 species of Traditional Chinese Medicinal Plants and Their Combined Prescriptions and Antibiotics

AgainstVibriofluvialis

XUXiaojin,LIXiuhua，MAYifan,HEJun,LIHuiyao,QIXin,ZHANGJiaonan,

WANGYuncai,ZHAOLinming,YANQingpi(343)

Effects of Combined Treatment with Microbial Fertilizer and Amino Acid Water-soluble Fertilizer on

Physicochemical Properties and Bacterial Communities Structure of Hami Melon Soils

QIZhao,ZHANGManli,YANZhen,XUMin,LIXianghuang,MAYe,XIONGRui,LIUXiaolei,TANGHua(352)

Antibacterial Mechanism of Tea Saponin AgainstSalmonellasp. and Its Application in Chicken Breast Preservation

HERongrong,TANYunshou,ZHANGMeihong,LIZhengxiang,ZHONGQiuping(360)

Guangdong Agribusiness Tropical Agriculture Research Institute Co., Ltd.

XUYangyu,LIUFuxiang,HONGYanbin,CHENXiaoping,LIHaifen,WENShijie,

LIXingyu,ZHOUGuiyuan,LIANGXuanqiang(367)

Isolation and Identification of Endophytic Fungi and Bacteria fromPhalaenopsisdeliciosa

CHENYaoli，YULongchun,QIANYue,SONGXiqiang,ZHANGZhe(372)

Evaluation of Land Productivity in Rubber Plantations Based on Data Mining

PENGJinlian,TANGZhaosheng,JIANGJusheng,ZHAOChunmei(380)

Soil Enzyme Activities and Their Influencing Factors in the Tropical Montane Rain Forest in the Rainy Season in Jianfengling,

Hainan

XUQuan,YANGHuai,YANGQiu,JIANGYamin,ZHANGShuqi,LIUWenjie(387)

Analysis of Arboreal Lichens Communities on Forest Tree Trunks in Coastal Hilly Land of Mountain

Tongguling, Wenchang, Hainan Province

GAOBoyu,YANGXiaobo,LIYingying,LIDonghai,ZHANGLi,SONGJiayu(396)

A Survey of Wild Plant Resources and Their Landscape Value in Baishiling Ridge in Hainan

SONGJiayu,YANGXiaobo,LIDonghai,ZHANGLi,GAOBoyu(406)

Evaluation of Correlation Between Vitality and Spatial Components of Open Space in Urban Streets

——With Haidian Island in Haikou City as a Case Study

DUANLei,LINShiping,CHENQiuchi,MAShanshui,ZHANGJie(417)

Layout of Tropical Urban Street Trees Based on Sunshine Requirements with Haikou City as an Example

WANGLi,KANGZihang,WUQingshu(424)

Preparation of Upgrading Strategy for Historical Arcade Streets in Haikou Based on Space Syntax

MAHao,DUANLei,FUHui,RENXiaoyu,XUXiansheng(432)