中图分类号R965 文献标志码A 文章编号1001-0408（2025）20-2525-06

DOI 10.6039/j.issn.1001-0408.2025.20.07

Improvement effects and mechanism of Bazheng powder on chronic urinary tract infection in rats

XI Huirong1，LI Xiaofeng²，JIANG Xiaolei1，LI Jing'，MA Zheng'an1，LI Xixiang1，²（1.Dept.of Pharmacy，Gansu Provincial Hospital of Traditional Chinese Medicine，Lanzhou 730050，China;2.Collge of Pharmacy，Gansu University of Chinese Medicine，Lanzhou 730o00，China）

ABSTRACTOBJECTIVETo investigate theimprovement efectsanditsmechanismof Bazheng powderonchronic urinarytract infection（CUTI）inducedbyEscherichiacoli inrats.METHoDs Therats weredivided into normalcontrolgroup，modelgroup， levofloxacin group （45mg/kg ）and Bazheng powder group （4.95g/kg） ，with 10 rats in each group. Except for the normal control group，other groups were administered an intravesical injection of Escherichia coli suspension （1×10⁸cfu/mL ）via the urethra to establish CUTImodel；atthesametime，rats ineach groupwereadministeredthecorresponding medicinal solutionorwaterby gavageonceadayfor4consecutive weks.Afterthelastmedication，bloodroutinetests（whitebloodcellcountandlymphocyte percentage），the levels of serum inflammatory factors [interleukin- 1β （IL-1β），IL-6，IL-8，tumor necrosis factor- α （TNF- α ）]， andimmune indicators[CD4，CD8，secretoryimmunoglobulinA（SIgA）]，renalfunctionindicators[cystatin C（Cys-C），α1- microglobulin（αl-MG），ureaandcreatinine]werealldetermined；thepathologicalchangesinrenalandbladdertisuesinrats were observed. The protein expressons of Toll-like receptor 4（TLR4），nuclear factor- κB 0 NF-κB ），and nucleotide-binding domain leucine-rich repeat and pyrin domain-containing receptor 3（NLRP3）inrat blader tisues were detected.RESULTS Compared with thenormal control group，the levels of IL 1β ，IL-6，IL-8，TNF- α ，CD8，Cys-C，αl-MG，ureaand creatinine in serum，aswell as the protein expressions of TLR4，NF- σ_κB andNLRP3in bladdertissues，were significantly elevated inthemodel group （ Plt;0.05 ）.Conversely，the levels of CD4 and SIgA were significantly decreased Plt;0.05 ）.Pathological changes，such as extensiveinfitrationof inflammatorycels，wereobservedinbothrenalandbladdertisues.Comparedwiththemodelgroup，the above quantitative indicators in the Bazheng powder group were significantly improved （ （Plt;0.05 ），with no obviousinflammatory lesionsobserved ineitherrenal or blader tisues.CONCLUSIONSBazheng powdercan aleviate inflammatoryreactionand

improvetheimmunefunctionof CUTIrats， andits mechanism mayberelatedtotheinhibition ofTLR4/NF- κB/ NLRP3 signaling pathway.

KEYWORDSBazheng powder； chronic urinary tract infection;inflammatory reaction；immune function;Escherichiacoli;TLR4/NF- ⋅κB， /NLRP3 signaling pathway

尿路感染是临床常见的泌尿系统感染性疾病，主要是由于细菌、真菌等病原体在尿路中异常生长、繁殖，侵犯黏膜或组织，继而引起的炎症性疾病。尿路感染根据病情可分为急性尿路感染和慢性尿路感染（chronicurinarytractinfection，CUTI），其中CUTI主要由急性感染久治不愈所致，病情反复且病程较长，若不及时治疗可诱发脓毒血症和肾衰竭，甚至危及患者生命安全[]。现代研究表明，大肠埃希菌是CUTI的常见致病菌，约占其致病菌总数的 70%～90% 3]。目前，临床治疗CUTI的一线药物为抗菌药物，但长期使用易出现细菌耐药的现象，而中药在治疗尿路感染方面具有独特的优势，可缓解尿路感染症状，并降低复发率[4。

八正散出自《太平惠民和剂局方》，是治疗湿热淋证的代表方，由瞿麦、篇蓄、车前子等多味中药组成，具有清热利湿、通淋止痛的功效，被广泛用于尿路感染、前列腺炎、膀胱炎等泌尿系统疾病的临床治疗[5-。研究发现，Toll样受体4（Toll-likereceptor4，TLR4）/核因子 κB （nuclear factor- κB ，NF- σ_κB ）/核苷酸结合结构域富含亮氨酸重复序列和含热蛋白结构域受体3（nucleotide-binding domain leucine-rich repeat and pyrin domain-containingreceptor3，NLRP3）信号通路在尿路感染相关炎症反应的发生中具有重要作用[-9。因此，调控上述信号通路可能是治疗尿路感染的重要策略。基于此，本研究以大肠埃希菌诱导构建CUTI大鼠模型，并基于TLR4/NF-kB/NLRP3信号通路探究八正散对该模型的改善作用及潜在机制，以期为八正散治疗尿路感染的科学内涵阐释及CUTI的临床治疗提供参考。

1材料

1.1 主要仪器

PocH-100iV型血细胞分析仪、KNF-100型尿液分析仪均购自希森美康医用电子（上海）有限公司；……