方园 黄河 刘迈兰 刘红华 冯芳 常小荣

〔摘要〕 目的 觀察隔药饼灸对动脉粥样硬化兔血清脂联素及血脂含量的影响。方法 将40只雄性新西兰兔随机分为正常组、模型组、隔药饼灸组、阿托伐他汀组，每组10只，模型组喂食高胆固醇饲料，阿托伐他汀组予高胆固醇饲料加阿托伐他汀喂养，隔药饼灸组喂食高胆固醇饲料同时予以隔药饼灸，12周后取材。采用ELISA法测定各组兔血清中总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-C）及血清脂联素（ADPN）含量;HE染色观察兔主动脉管壁形态结构。结果 干预后，与正常组比较，模型组TC、TG、LDL-C均明显升高（P<0.01），ADPN水平明显降低（P<0.01）。与模型组比较，隔药饼灸组、阿托伐他汀组TC、TG、LDL-C均不同程度降低（P<0.05，P<0.01），隔药饼灸组ADPN含量升高（P<0.05），阿托伐他汀组ADPN水平升高，但差异无统计学意义（P>0.05）。与正常组比较，模型组主动脉管壁结构明显受损;与模型组比较，隔药饼灸组、阿托伐他汀组主动脉内皮结构明显改善。结论 隔药饼灸抗动脉粥样硬化的作用机制，可能与调节血清ADPN含量、降低血脂水平、改善动脉管壁损伤情况以及防治动脉粥样硬化斑块的形成有关。

〔关键词〕 动脉粥样硬化;隔药饼灸;脂联素;总胆固醇;甘油三酯;低密度脂蛋白胆固醇

〔中图分类号〕R245.8       〔文献标志码〕A       〔文章编号〕doi：10.3969/j.issn.1674-070X.2021.10.001

Effect of Herbal Cake-separated Moxibustion on Serum Adiponectin and Blood

Lipid Levels in Rabbits with Atherosclerosis

FANG Yuan1， HUANG He1， LIU Mailan1， LIU Honghua1， FENG Fang2*， CHANG Xiaorong1*

（1. Hunan University of Chinese Medicine， Changsha， Hunan 410000， China;

2. West Hospital of Chenzhou First Peoples Hospital， Chenzhou， Hunan 423000， China）

〔Abstract〕 Objective To observe the effect of herbal cake-separated moxibustion on serum adiponectin and lipid content of atherosclerotic rabbits. Methods 40 male New Zealand rabbits were randomly divided into normal group， model group， herbal cake-separated moxibustion group and atorvastatin group， with 10 rabbits in each group. The model group was fed with high-cholesterol diet， the atorvastatin group was fed with high-cholesterol diet plus atorvastatin， and the herbal cake-separated moxibustion group was fed with high-cholesterol diet and given herbal cake-separated moxibustion， and samples were taken after 12 weeks. The content of serum total cholesterol （TC）， triglyceride （TG）， low density lipoprotein cholesterol （LDL-C） and serum adiponectin （ADPN） were measured by ELISA. HE staining was used to observe the morphology and structure of rabbit aortic wall. Results After intervention， TC， TG and LDL-C in the model group were significantly higher than those in the normal group （P<0.01）， the serum ADPN level in the model group decreased significantly  （P<0.01）. Compared with the model group， TC， TG and LDL-C in herbal cake-separated

moxibustion group and atorvastatin group were all decreased to different degrees （P<0.01， P<0.05）， the serum ADPN content in the herbal cake-separated moxibustion group increased （P<0.05）， the serum ADPN level in atorvastatin group increased， but the difference was not statistically significant  （P>0.05）. Compared with the normal group， the aortic wall structure of the model group was

significantly damaged， and compared with the model group， the aortic endothelial structure of the herbal cake-separated moxibustion group and atorvastatin group was significantly improved. Conclusion The anti-atherosclerotic mechanism of herbal cake-separated

moxibustion may be related to regulating the content of serum ADPN， reducing the level of blood lipid， improving the injury of arterial wall， and preventing and treating the formation of atherosclerotic plaque.

〔Keywords〕 atherosclerotic; herbal cake-separated moxibustion; adiponectin; total cholesterol; triglyceride; low density lipoprotein cholesterol

以动脉粥样硬化（atherosclerosis， AS）为诱因的心血管疾病（cardiovascular diseases， CVD）是造成人类死亡的常见原因，据《中国心血管健康与疾病报告2020概要》中分析，至2018年心血管疾病的患病人数已达3.30亿[1]。AS是大中动脉血管壁内脂质驱动的慢性炎症疾病，脂质代谢异常是AS发生发展关键因素之一，不仅可促进AS发展，还可加速斑块形成[2-4]。因此，调控脂质代谢是临床防治AS的重要环节。血清脂联素（adiponectin， ADPN）主要是由脂肪细胞分泌的一种内源性生物活性蛋白质，在体外作为一种激素可减少炎症反应[5]，具有抗氧化、抗动脉粥樣硬化、促血管生成和胰岛素增敏等有益代谢的特性[6]。……