促性腺激素释放激素类似物和左旋多巴对秀丽高原鳅体长、体重和卵巢发育的影响
2021-08-26黄松茂白华毅李光华于虹漫冷云毕保良王正阳赵玉姣张铭枭孔令富
黄松茂 白华毅 李光华 于虹漫 冷云 毕保良 王正阳 赵玉姣 张铭枭 孔令富



摘要:秀丽高原鳅(Triplophysa venusta)为云南特有鱼类,为阐明促性腺激素释放激素类似物和左旋多巴对秀丽高原鳅体长、体重和卵巢发育的影响,本实验采用生物化学等技术,研究了促性腺激素释放激素类似物(GnRH-a,0.1 mg/kg/2周,共注射4次)和左旋多巴(L-dopa,100 mg/kg/2周,共注射4次)对体长、体重和卵巢发育的影响,以期为秀丽高原鳅人工繁育提供理论依据。结果表明:两种外源激素处理组和对照组间体长、体重变化均无显著差异,两种外源激素对秀丽高原鳅体长和体重的影响不明显;由于个体成熟度差异较大,有的个体卵巢尚未发育成熟,故无法进行分区的组间比较,但对少数成熟个体进行了卵巢腺发育的特征和组织学分期。
关键词:秀丽高原鳅;外源激素;体长、体重;卵巢发育
中图分类号:S917 文献标识码:A
文章编号:1006-3188(2021)03-007-06
Abstract: Triplophysa venusta is a fish unique to Yunnan.In order to clarify the effects of gonadotropin-releasing hormone analogues and levodopa on the body length, weight and ovarian development of Triplophysa venusta, this experiment used biochemical techniques to study Gonadotropin-releasing hormone analogues (GnRH-a, 0.1 mg/kg/2 weeks, 4 injections total) and levodopa (L-dopa, 100 mg/kg/2 weeks, 4 injections total) The effects of body length, weight and ovarian development are expected to provide a theoretical basis for the artificial breeding of Triplophysa venusta.The results showed that there was no significant difference in body length and weight between the two exogenous hormone treatment groups and the control group, and the two exogenous hormones had no significant effect on the body length and weight of Triplophysa venusta; due to the difference in individual maturity The ovaries of some individuals are not yet mature, so it is not possible to compare the divisions between groups.However, a few mature individuals were given the characteristics of ovarian gland development and histological staging.
Keywords: Triplophysa venusta; exogenous hormones; body length and weight; ovarian developmentt
秀麗高原鳅(Triplophysa venusta)属软骨鱼纲(Chondrichthyes)、鲤形目(Cypriniformes) 、条鳅科(Nemacheilidae),为云南省特有品种,仅分布于云南金沙江中、上游干支流和附属水体(包括湖泊)[1]。秀丽高原鳅为均匀型生长鱼类,雌雄个体体长、体重和肥满度无显著差异[2],是一种高蛋白、高脂肪,且富含氨基酸和脂肪酸的营养均衡、肉味鲜美的鱼类[3]。幼鱼适合生存pH值为4.55~10.15[4],耗氧率随温度降低而减小,昼夜变化不明显[5],在人工可控条件下可存活并生长[6]。但关于外源激素对其体长、体重和卵巢发育的影响尚不明确。
本研究采用腹腔注射促性腺激素释放激素类似物(GnRH-a)和左旋多……
