谢玲 陶菡 张学俊 张灵丽 贺扬洁 田运霞 吴巧灵 季春

中圖分类号 R284.2 文献标志码 A 文章编号 1001-0408（2021）13-1557-08

DOI 10.6039/j.issn.1001-0408.2021.13.04

摘 要 目的：优化杜仲翅果皮酶解提取液的超滤工艺。方法：采用单因素实验考察超滤膜截留分子量、料液温度、操作压力、操作频率、过膜时间、料液浓度、料液pH对杜仲翅果皮酶解提取液中桃叶珊瑚苷、京尼平苷酸、绿原酸的转移率和固形物去除率的影响。固定超滤膜截留分子量100 000、料液浓度7 g/L、料液pH 7，以料液温度、操作压力、操作频率、过膜时间为考察因素，以桃叶珊瑚苷、京尼平苷酸、绿原酸的转移率和固形物去除率的综合得分为评价指标，采用Box-Behnken设计-响应面法对超滤工艺进行优化并验证。结果：杜仲翅果皮酶解提取液的最佳超滤工艺为料液温度35 ℃、操作压力0.5 MPa、操作频率35 Hz、过膜时间42 min。验证试验结果显示，杜仲翅果皮酶解提取液中桃叶珊瑚苷、京尼平苷酸、绿原酸的转移率和固形物去除率的综合得分为78.06%（RSD=1.43%，n=3），与预测值（77.18%）的相对误差为1.14%。结论：优化后的超滤工艺稳定、可靠，可用于杜仲翅果皮酶解提取液的超滤纯化。

关键词 杜仲翅果皮;超滤工艺;Box-Behnken设计;响应面法;桃叶珊瑚苷;京尼平苷酸;绿原酸

Optimization of Ultrafiltration Technology of Enzymatic Hydrolysate from Eucommia ulmoides Peel

XIE Ling1，TAO Han1，ZHANG Xuejun1，ZHANG Lingli1，HE Yangjie1，TIAN Yunxia1，WU Qiaoling1，JI Chun2   （1. Guizhou Province Key Laboratory of Fermentation Engineering and Bio-Pharmaceutical/School of Liquor & Food Engineering， Guizhou University， Guiyang 550025， China; 2. School of Pharmaceutical Sciences， Guizhou University， Guiyang 550025， China）

ABSTRACT   OBJECTIVE： To optimize the ultrafiltration technology of enzymatic hydrolysate from Eucommia ulmoides peel. METHODS： The single factor test was adopted to investigate the effects of molecular weight of ultrafiltration membrane， liquid temperature， operating pressure， operating frequency， membrane filtration time， liquid concentration and pH on transfer rates of aucubin， geniposide and chlorogenic acid as well as solid removal rate in enzymatic hydrolysate from E. ulmoides peel. Setting the molecular cut off of fixed ultrafiltration membrane of 100 000， liquid concentration of 7 g/L， and pH value of 7， the ultrafiltration technology was optimized by Box-Behnken design response-surface methodology and validated with liquid temperature， operating pressure， operating frequency and membrane passing time as factors， using comprehensive scores calculated from transfer rates of aucubin， geniposide and chlorogenic acid as well as solid removal rate as indexes. RESULTS： The optimal ultrafiltration technology of enzymatic hydrolysate from E. ulmoides peel was as follows as liquid temperature of 35 ℃， operating pressure of 0.5 MPa， operating frequency of 35 Hz and membrane passing time of 42 min.  Results of validation tests showed that the comprehensive scores of the transfer rates of aucubin， geniposide and chlorogenic acid as well as solid removal rate in enzymatic hydrolysate from E. ulmoides peel was 78.06% （RSD=1.43%， n=3）， and its relative error with the predicted value （77.18%） was 1.14%. CONCLUSIONS： The optimized ultrafiltration technology is stable and reliable， and can be used for the ultrafiltration purification of enzymatic hydrolysate from E. ulmoides peel.

KEYWORDS   Eucommia ulmoides peel; Ultrafiltration technology; Box-Behnken design; Response surface methodology; Aucubin; Geniposidic acid; Chlorogenic acid

杜仲Eucommia ulmoides Oliv.属于被子植物门杜仲科Eucommiaceae，是我国重要的名贵中药材之一，传统以杜仲皮入药外，其叶、雄花、果实也具有很高的药用价值，在《神农本草经》《本草纲目》《中国药典》中均有详细记载[1-2]。杜仲翅果皮是杜仲果实的皮，其中含有环烯醚萜类、酚酸类、萜类、甾体类、苯丙素类等活性成分[3]。其中，苯丙……