窦金金 齐珊 张喜武

摘要 目的：观察加减黄连温胆汤对血管性痴呆（Vascular Dementia，VD）模型大鼠脑组织白细胞介素-1β（Interleukin-1β，IL-1β）mRNA、肿瘤坏死因子-α（Tumor Necrosis Factor-α，TNF-α）mRNA和环氧合酶-2（Cyclooxygenase-2，COX-2）mRNA的影响，明确炎症反应对VD的影响，探讨加减黄连温胆汤对VD大鼠的炎症作用机制。方法：采用大鼠双侧颈总动脉间断性结扎的方法，复制VD大鼠模型。通过逆转录-聚合酶链反应（Reverse Transcription-polymerase Chain Reaction，RT-PCR）法试剂盒检测TNF-αmRNA、IL-1βmRNA和COX-2 mRNA，并描绘各验证因子数据表达变化。結果：模型组与空白组、假手术组比较，模型组脑组织IL-1βmRNA和TNF-αmRNA及COX-2mRNA的表达明显增多，有显著性差异;加减黄连温胆汤与模型组比较，加减黄连温胆汤可使IL-1βmRNA和TNF-αmRNA及COX-2mRNA的表达下调，有显著性差异。结论：加减黄连温胆汤可通过抑制炎症介质IL-1βmRNA、TNF-αmRNA及COX-2 mRNA的表达，发挥对VD模型大鼠的治疗作用。

关键词 加减黄连温胆汤;血管性痴呆;炎症反应;逆转录-聚合酶链反应;白细胞介素-1βmRNA;肿瘤坏死因子-αmRNA;环氧合酶-2 mRNA

Abstract Objective：To observe the effects of modified Huanglian Wendan Decoction on interleukin-1β（IL-1β）mRNA，tumor necrosis factor-α（TNF-α） mRNA and cyclooxygenase-2（COX-2）mRNA in the brain tissue of vascular dementia（VD）model rats.To clarify the influence of inflammation on VD，and to explore the inflammatory mechanism of modified Huanglian Wendan Decoction on VD rats.Methods：The rat model of VD was replicated by intermittent ligation of bilateral common carotid arteries in rats.TNF-αmRNA，IL-1βmRNA and COX-2 mRNA were detected by reverse transcription-polymerase chain reaction（RT-PCR）kit，and the expression changes of each verification factor data were depicted.Results：Compared with the blank group and the sham operation group，the expression of IL-1βmRNA，TNF-αmRNA and COX-2mRNA in the brain tissue of the model group was significantly increased，and there was a significant difference.Compared with the model group，modified Huanglian Wendan Decoction，can down-regulate the expression of IL-1βmRNA，TNF-αmRNA and COX-2mRNA，with significant differences.Conclusion：Modified Huanglian Wendan can reduce the inhibition of inflammatory factor the expression of IL-1βmRNA，TNF-αmRNA and COX-2mRNA，and play a role in inhibiting inflammation.

Keywords Modified Huanglian Wendan Decoction; Vascular dementia; Inflammation reverse transcription-polymerase chain reaction; Interleukin-1βmRNA; Tumor necrosisfactor-α; TNF-αmRNA; Cyclooxygenase-2mRNA

中图分类号：R289.5文献标识码：Adoi：10.3969/j.issn.1673-7202.2021.09.012

血管性痴呆指由各类脑血管疾病导致的脑功能障碍而产生的一种获得性智能损害综合征[1]，患病者有不同程度的记忆、认知、行为等认知障碍[2]。中药方剂可通过抑制神经细胞凋亡、改善血流变、调节神经递质、激活脑细胞、抗氧化损伤等作用治疗VD，尤其是抑制炎症反应作用[3-4]。众多中医大家普遍认为VD病机为髓海虚空、脏腑衰退、痰瘀互结、浊毒化生，导致脑络痹阻、络脉瘀滞、神志失统[5-7]。黄连温胆汤是化痰的经典方，在此基础上加用活血化瘀类药物，可共奏化痰活血之功。本研究应用RT-PCR等技术，在VD模型基础上完成VD模型大鼠炎症反应影响研究，以利于指导临床应用，以期为VD的治疗提供新思路。……