吕邵娃 邱茜 杨志伟 李庆华 王秋红 匡海学

〔摘要〕 目的 研究婦康栓对妇科疾病致病菌的抑菌活性及对厌氧消化链球菌生物膜的影响。方法 采用滤纸片法测量供试药液作用于菌株的抑菌圈直径;采用倍比稀释法检测菌株的最小抑菌浓度（minimum bacteriostatic concentration， MIC）及最低杀菌浓度（minimum bactericidal concentration， MBC）;采用荧光染色法对厌氧消化链球菌生物膜的形成抑制情况进行红绿荧光定量分析。结果 妇康栓对白色念珠菌、新生隐球菌、金黄色葡萄球菌、大肠埃希氏菌、厌氧消化链球菌、乙型溶血性链球菌、红色毛癣菌及阴道加德纳氏菌均有不同程度的抑制作用，尤其是对革兰氏阳性菌的抑制作用强于其他菌株。抑菌结果表明药物对厌氧消化链球菌的抑制作用最强。在荧光显微镜下可清楚观察，与对照组相比，各给药组绿色荧光百分比减少，生物膜总量降低（P<0.05）。结论 妇康栓具有抑菌活性，特别是对厌氧消化链球菌具有较强的抑制作用，并具有破坏生物膜的能力。表明妇康栓对妇科疾病主要致病菌有抑制作用，为进一步临床用药提供理论指导。

〔关键词〕 妇康栓;体外抑菌;妇科炎症;厌氧消化链球菌;生物膜

〔中图分类号〕R285.5       〔文献标志码〕A       〔文章编号〕doi：10.3969/j.issn.1674-070X.2021.06.004

Study on Antibacterial Activity in Vitro and Effect of Fukang Suppository on Bacterial Biofilm

LV Shaowa1，2， QIU Qian1，2， YANG Zhiwei3， LI Qinghua3， WANG Qiuhong1，4， KUANG Haixue1，2*

（1. Key Laboratory of Basic and Applied Research of Northern Medicine， Ministry of Education， Heilongjiang University of Traditional Chinese Medicine， Harbin， Heilongjiang 150040， China; 2. Key Laboratory of Basic Research on Pharmacodynamic Substances of Traditional Chinese Medicine and Natural Medicine， Heilongjiang University of Traditional Chinese Medicine， Harbin， Heilongjiang 150040， China; 3. Heilongjiang Province Rich East Pharmaceutical Company Limited， Mudanjiang

157000， Heilongjiang， China; 4. College of Traditional Chinese Medicine， Guangdong Pharmaceutical University， Guangzhou， GuangDong 510000， China）

〔Abstract〕 Objective To study the antibacterial activity of Fukang Suppository on pathogenic bacteria of gynecological diseases and its effect on the biofilm of anaerobic digestion streptococcus. Methods The diameters of inhibition zone of test solution to bacteria was measured by the paper filtering method. The minimum bacteriostatic concentration （MIC） and minimum bactericidal

concentration （MBC） of Fukang Suppository were determined by dilution method. The inhibition of biofilm formation in anaerobic digestion streptococcus was quantitatively analyzed by fluorescence staining. Results Fukang Suppository had different

degree of inhibitory effect on candida albicans， cryptococcus neoforme， staphylococcus aureus， escherichia coli， streptococcus anaerobic digestion， streptococcus hemolytis-β， trichophyton rubrum and Gardnerella vaginalis. The inhibitory effect of the drug on the gram positive bacteria was the strongest. The results of bacteriostasis showed that the drug had the strongest inhibitory effect on anaerobic digestion streptococcus. It can be observed clearly under fluorescence microscope， compared with the control group， the percentage of green fluorescence and the total amount of biofilm in each group were decreased （P<0.05）. Conclusion Fukang Suppository had antibacterial activity， especially against the anaerobic digestion streptococcus， and had the ability to destroy the biofilm. It is proved that Fukang Suppository has an inhibitory effect on the main pathogenic bacteria of gynecological diseases， which provides theoretical guidance for further clinical medication.

〔Keywords〕 Fukang Suppository; inhibition in vitro; gynecological inflammation; anaerobic digestion streptococcus; biological membrane

妇康栓是以核桃楸（Juglans mandshurica Maxim.）的成熟种仁采用干馏法得到的馏油为主药，硬脂酸聚烃氧40酯为基质，混合制成黑褐色鸭嘴形栓剂。核桃楸中含有醌、黄酮、糖苷、鞣质和三萜类化合物[1]，具有抗肿瘤、抗氧化、杀菌和止痛等多种作用[2-3]。从核桃楸外果皮提取分离出的活性成分对多种病原微生物有抑制作用，然而关于核桃楸成熟种仁的研究报道较少[4]。妇康栓作为阴道外用药用于治疗赤白带下、少腹坠痛、子宫糜烂等多种常见妇科疾病，但其是否通过抑菌起到对宫颈炎、阴道炎及盆腔炎的治疗作用尚不清楚。……