张子轩 陈奕州 白瑞 李梅兰 张静

摘 要：为比较不同萝卜品种及同一品种萝卜肉质根不同部位的硫代葡萄糖苷含量，以11个品种萝卜为试材，将萝卜肉质根分为上中下三部分，采用氯化钯-分光光度法对总硫苷含量进行测定。结果表明，11个萝卜品种肉质根中总硫苷含量有一定差 异，其中甜脆水果萝卜肉质根中总硫苷含量（w，后同）最高，为24.45 mg·g-1;不同品种萝卜同一部位的总硫苷含量也不同，甜脆水果萝卜肉质根上部和中部的总硫苷含量最高;特选白蔓菁萝卜肉质根下部总硫苷含量最高;同一萝卜品种不同部位的总硫苷含量存在差异。以上研究结果为筛选出高硫代葡萄糖苷药食两用萝卜品种提供了依据，为选育优质萝卜品种提供了一定的理论支撑。

关键词：萝卜;总硫代葡萄糖苷;含量

中图分类号：S631.1 文献标志码：A 文章编号：1673-2871（2021）05-064-04

Content analysis of total glucosinolates in different radish varieties

ZHANG Zixuan1， CHEN Yizhou1， BAI Rui1， LI Meilan1， 2， ZHANG Jing1， 2

（1. College of Horticultural Science， Shanxi Agricultural University， Taigu 030801， Shanxi， China; 2. Collaborative Innovation Center of Improving quality and Increasing profits for Protected Vegetables in Shanxi， Taigu 030801， Shanxi， China）

Abstract： In order to compare the total glucosinolates contents of fleshy roots in different radish varieties and different parts of the same radish variety， eleven radish varieties were used as materials， such as Fengqiaoyidai， Tiancui Shuiguo， Sanchibai and so on， ， the total glucosinolates content was determined by Palladium chloride-Spectrophotometer. The results showed that the glucosinolates contents in the fleshy roots of eleven radish varieties were different， and the total glucosinolates content of the fleshy roots in Tiancui Shuiguo radish was the highest （24.45 mg·g-1 DW）. Moreover， the total glucosinolates contents of the same part in different radish varieties were also different. The total glucosinolates contents of the upper and middle parts of Tiancui Shuiguo were the highest. The total glucosinolates content of the lower part of Texuan Baimanqing was the highest. In addition， the total glucosinolates contents of different parts in the same radish variety were different. The results not only provided a basis for screening medicinal and edible radish varieties with high glucosinolates， but also provided a theoretical support for the breeding of high quality radish variety.

Key words： Radish; Total glucosinolates; Content

蘿卜（Raphanus stivus L.）为十字花科（Cruciferae）萝卜属（Raphanus）一、二年生草本植物，栽培历史悠久，是重要的蔬菜作物[1]。萝卜的肉质根发达，主要由次生木质部构成，为主要的食用器官，具有较高的药用和食用价值。十字花科蔬菜中含有多种营养物质，如糖类、维生素类和萝卜硫素等，多项研究表明萝卜硫素具有显著的抗癌活性[2]。

硫代葡萄糖苷（glucosinolate，GLs，简称硫苷）是十字花科植物中一种重要的次生代谢产物[3]，是抗癌化合物的前体物质，是最主要的抗癌活性物质，对食道癌、直肠癌、乳腺癌、肺癌和胃癌等有抗性[4]。除此之外，硫苷在植物的风味品质调节以及植物抗虫、抗逆性等多个方面有显著的作用，是植物防御体系中的重要组成部分[5-6]。……