吴晓玲　　李力强　　段文彪　　吴伟斌　　杨坚　　胡钰颖

[摘要] 目的 探讨活血消癥益气方对肝硬化门静脉高压症模型大鼠门静脉压力、肝功能及血清PDGF、TGF-β1、CTGF的影响及其作用机制。 方法 采用四氯化碳复合法诱导构建肝硬化门静脉高压症大鼠模型，然后采用普萘洛尔及活血消癥益气方分别干预治疗8周。治疗结束后采用直接穿刺门静脉法测定大鼠的门静脉压力，取肝组织采用HE及Masson染色观察肝组织纤维化及门静脉曲张情况，取下腔静脉血检测大鼠肝功能及肝纤维化相关因子的水平。 结果 ①活血消癥益气方能显著改善纤维化大鼠的体重下降。②活血消癥益气方能显著改善肝组织纤维化、重建正常肝小叶，减轻肝细胞炎症浸润程度，减少结缔组织增生，肝细胞索排列基本整齐。③活血消癥益气方可显著改善肝纤维化大鼠的肝功能情况（P<0.05）。④活血消癥益气方能显著降低肝纤维化大鼠血清PDGF、TGF-β1、CTGF等纤维化相关因子及门静脉压力的水平（P<0.05）。 结论 活血消癥益气方能有效改善肝硬化门静脉高压症模型大鼠门静脉压力、肝功能损伤、肝纤维化，作用机制可能与调控血清PDGF、TGF-β1、CTGF水平有关。

[关键词] 活血消癥益气方;肝硬化门静脉高压症;血小板衍生生長因子;转化生长因子-β1;结缔组织生长因子

[中图分类号] R965.1          [文献标识码] A          [文章编号] 1673-9701（2021）13-0037-05

The effect of Huoxue Xiaozheng Yiqi Recipe on liver function and serum PDGF，TGF-β1， CTGF in rats with cirrhosis portal hypertension

WU Xiaoling   LI Liqiang   DUAN Wenbiao   WU Weibin   YANG Jian   HU Yuying

Zhaoqing Medical College， Zhaoqing   527400， China

[Abstract] Objective To investigate the effects of Huoxue Xiaozheng Yiqi Recipe on portal venous pressure，liver function，serum PDGF， TGF-β1， and CTGF in cirrhosis portal hypertension model rats and the mechanism of action. Methods The rat model of cirrhosis portal hypertension was induced by the carbon tetrachloride complex method，and then the rats were respectively interfered with propranolol and Huoxue Xiaozheng Yiqi Recipe for eight weeks. After treatment， the portal vein pressure of rats was measured by the direct portal vein puncture method. Liver tissues were taken to observe liver fibrosis and portal varicose vein by HE and Masson staining. The lower venous blood in rats was taken to detect liver function and the level of hepatic fibrosis-related factors. Results （1）Huoxue Xiaozheng Yiqi Recipe significantly improved the weight loss of rats with fibrosis. （2）Huoxue Xiaozheng Yiqi Recipe can significantly improve liver tissue fibrosis， rebuild normal liver lobules， reduce the degree of inflammatory infiltration of liver cells， reduce the proliferation of connective tissue， and the arrangement of liver cell cord was basically orderly. （3）Huoxue Xiaozheng Yiqi Recipe significantly improved the liver function of liver fibrosis rats （P<0.05）. （4）Huoxue Xiaozheng Yiqi Recipe significantly reduced serum levels of fibrosis-related factors such as PDGF， TGF-β1， CTGF， and portal venous pressure in hepatic fibrosis rats（P<0.05）. Conclusion Huoxue Xiaozheng Yiqi Recipe can effectively improve portal venous pressure， liver function damage， and liver fibrosis in cirrhosis portal hypertension model rats，and the mechanism of action may be related to regulating serum levels of PDGF， TGF-β1， and CTGF.

[Key words] Huoxue Xiaozheng Yiqi Recipe; Cirrhosis portal hypertension; Platelet derived growth factor;Transforming growth factor-β1; Connective tissue growth factor

门静脉高压症（Portal hypertension，PH）是一组以门静脉血流系统异常变化为特点的症候群，各种原因所致的肝硬化为其最常见病因，食管胃底曲张静脉破裂出血为门脉高压症的主要并发症[1]。近50%门静脉高压症患者可出现食管胃底静脉曲张，发生率与肝功能损害的严重程度有关[2]。门静脉高压症的治疗方法包括药物、介入、内镜和手术治疗等，门静脉高压症应遵循个性化治疗原则，筛选能改变纤维化发展过程、应用于肝硬化早期及肝纤维化患者的药物是关键[3]。……