张鑫 刘阳 姜巍 王垂杰 李玉锋

摘要：目的 研究肝郁型功能性消化不良大鼠胃排空率以及胃和下丘腦组织Ghrelin 蛋白的表达变化，以探究和胃理气方对功能性消化不良干预的可能机制。方法 选取40只健康Wistar大鼠，雌雄各半，体质量（200±20）g，随机分为空白组（10只）及模型组（30只）。空白组常规饲养，其余大鼠通过夹尾刺激法建立肝郁型FD大鼠模型，造模14 d。造模结束后按照随机数字表分为模型组、吗丁啉组、和胃理气方组（每组10只）。期间监测大鼠进食以及毛发、行为等一般状况。实验结束后，测量各组大鼠胃排空率，并通过Western blot法测定胃组织以及下丘脑组织Ghrelin蛋白表达。结果 模型组胃排空率明显低于和胃理气方组、吗丁啉组（P<0.05）;与模型组相比，和胃理气方组和吗丁啉组的胃及下丘脑Ghrelin蛋白表达增加（P<0.05）。结论 和胃理气方可能通过上调Ghrelin蛋白表达来有效干预功能性消化不良。

关键词：功能性消化不良;脑肠互动;胃;下丘脑;Ghrelin

中图分类号：R285.5 文献标志码：A 文章编号：1007-2349（2021）04-0063-04

Effect of Hewei Liqi Decoction on Ghrelin Expression in Rats withLiver-Stagnation Type of Functional Dyspepsia

ZHANG Xin1， LIU Yang1， JIANG Wei2， WANG Chui-jie2， LI Yu-feng2

（1. Liaoning University of Traditional Chinese Medicine， Shenyang 110032， China;2. The Affiliated Hospital of Liaoning University of Traditional Chinese Medicine， Shenyang 110032， China）

【Abstract】Objective： To study the gastric emptying rate and the expression changes of Ghrelin protein in stomach and hypothalamus in rats with liver-stagnation functional dyspepsia in order to explore the possible mechanism of Hewei Liqi decoction on functional dyspepsia. Methods： 40 healthy Wistar rats were selected， half male and half male， and weighed （200±20） g. They were randomly divided into a blank group （10） and a model group （30）. The blank group was bred routinely and the rest of the rats were used to create a liver depression type FD rat model by the tail-clamping method， and the model was established for 14 days. After modeling， the rats were divided into model group， domperidone group and Hewei Liqi Decoction group （10 each group） according to the random number table. During this period， the general conditions of the rats' food intake， hair， behavior， etc. were monitored. After the experiment， the gastric emptying rate of rats in each group was measured and the expression of Ghrelin protein in gastric tissue and hypothalamic tissue was determined by Western blot. Results： The gastric emptying rate of the model group was significantly lower than that of the Hewei Liqi Decoction group and the domperidone group（P<0.05）. Compared with that of the model group， the gastric and hypothalamic Ghrelin protein expression in the Hewei Liqi Decotion group and the domperidone group increased （P<0.05）. Conclusion： Hewei Liqi Decoction may effectively interfere with functional dyspepsia by up-regulating the expression of Ghrelin protein.

【Key words】Functional dyspepsia;Brain-intestine interaction;Stomach;Hypothalamus;Ghrelin

功能性消化不良（functional gastriointestinal disease，FD）是全球范围内常见的功能性胃肠病之一，全球各国的消化不良患病率相差较大，总患病率约为11.5%～15.5%[1]。目前認为它是一种生物-心理-社会病症，其病因和发病机制还不是十分清楚，多考虑与遗传易感性、胃肠运动障碍、内脏敏感性增高、幽门螺杆菌（Helicobacter pylori，H.pylori）感染、中枢神经系统失调、黏膜免疫和炎症反应、脑肠轴异常及精神-心理社会因素等有关[2]。近年来随着对神经内分泌学说的深入研究，越来越多的学者认为脑肠轴在FD的发病中起着重要的作用[3]。由精神心理因素导致的FD患者除了有上腹部不适，早饱等胃肠道症状以外，还伴随着不同程度的焦虑紧张，而脑肠肽在此过程中有至关重要的作用。……