陈强锋 杨均秀 黎子武

【摘要】 目的：探討嗜酸乳杆菌对坏死性小肠结肠炎患儿免疫功能与肠道菌群的影响。方法：选取2015年1月-2020年10月在本院诊治的坏死性小肠结肠炎患儿72例为研究对象，根据随机信封1∶1抽签原则将患儿分为研究组与对照组，各36例。对照组给予美沙拉嗪治疗，研究组在对照组的基础上给予嗜酸乳杆菌治疗，两组均治疗3个月，检测患儿免疫功能与肠道菌群的变化情况。结果：研究组总有效率为86.11%，虽高于对照组的72.22%，但差异无统计学意义（P>0.05）。治疗后，研究组双歧杆菌、乳杆菌含量均高于对照组（P<0.05），肠杆菌、肠球菌含量均低于对照组（P<0.05）。治疗后，两组血清白介素-6（IL-6）、IL-8含量均低于治疗前（P<0.05），且研究组治疗后IL-6、IL-8含量均低于对照组（P<0.05）。治疗后两组CD4+T淋巴细胞比例均高于治疗前（P<0.05），研究组治疗后CD4+T淋巴细胞比例高于对照组（P<0.05），治疗前后两组CD3+T淋巴细胞比例在组内与组间比较，差异均无统计学意义（P>0.05）。结论：嗜酸乳杆菌在坏死性小肠结肠炎患儿的应用中能改善免疫功能，有利于维持肠道菌群平衡，从而抑制炎症因子的表达，提高治疗效果。

【关键词】 嗜酸乳杆菌 坏死性小肠结肠炎 免疫功能 肠道菌群 美沙拉嗪 炎症因子

Effects of Lactobacillus Acidophilus on Immune Function and Intestinal Flora in Children with Necrotizing Enterocolitis/CHEN Qiangfeng， YANG Junxiu， LI Ziwu. //Medical Innovation of China， 2021， 18（33）： -122

[Abstract] Objective： To investigate the effects of Lactobacillus acidophilus on the immune function and intestinal flora of children with necrotizing enterocolitis. Method： A total of 72 cases with necrotizing enterocolitis who were diagnosed and treated in our hospital from January 2015 to October 2020 were selected as the research objects. All the children were divided into the study group and the control group with 36 children in each groups accorded to the random envelope 1∶1 lottery principle. The control group were treated with Mesalazine， and the study group were treated with Lactobacillus acidophilus on the basis of the control group. Both groups were treated for 3 months， the changes in the immune function and intestinal flora of the children were detected. Result： The total effective rates of the study group were 86.11%， which were higher than 72.22% of the control group， but the difference was not statistically significant （P>0.05）. After treatment， the contents of Bifidobacterium and Lactobacillus in the study group were higher than those in the control group （P<0.05）， and the contents of Enterobacter and Enterococcus were lower than those in the control group （P<0.05）. The levels of serum interleukin-6 （IL-6） and IL-8 after treatment in the two groups were lower than those before treatment （P<0.05）， and those in the study group were lower than those in the control group after treatment（P<0.05）. The ratio of CD4+T lymphocytes in the two groups after treatment were higher than those before treatment （P<0.05）， and that in the study group was higher than that in the control group （P<0.05）. The ratio of CD3+T lymphocytes in the two groups before and after treatment were compared within and between groups， the difference were not statistically significant （P>0.05）. Conclusion： The application of Lactobacillus acidophilus in children with necrotizing enterocolitis can improve immune function and maintain the balance of intestinal flora， thereby inhibiting the expression of inflammatory factors and improving the therapeutic effect.

[Key words] Lactobacillus acidophilus Necrotizing enterocolitis Immune function Intestinal flora Mesalazine Inflammatory factors

First-author’s address： Foshan Fosun Chancheng Hospital Co.， Ltd， Foshan 528000， China

doi：10.3969/j.issn.1674-4985.2021.33.029

坏死性小肠结肠炎在临床上比较常见，也是较为严重的胃肠道急症之一，多发病于小儿[1-2]。随着人们饮食结构的改变和生活水平的提高，坏死性小肠结肠炎的发病人数呈上升趋势，且该病易反复发作，对患儿身心健康造成严重负面影响[3]。该病在临床上主要表现为血便、腹胀、呕吐、腹泻等症状，若未及时给予对症治疗，容易导致患儿出现呼吸衰竭、休克症状。……