柏静萍 宋志敏 魏宁颐 杨梅

摘要：目的 通过观测云南春燥环境条件下小鼠气道中粘多糖（RS）、白介素8（IL-8）含量的变化，探究春燥环境对小鼠气道津液代谢及卫外功能的影响，并阐释“燥胜则干”、“肺津耗伤”、“燥犯肺卫”、“肺卫失司”等中医理论。方法 复制云南春燥环境的多重因素作用于实验小鼠，并以桑杏汤灌胃反证模型。结果 模型组小鼠气道RS含量相較于正常组有显著降低（P<0.01）;加水组小鼠气道RS含量有明显下降（P<0.05）;桑杏汤组小鼠气道RS相较模型组含量有显著提升（P<0.01）;模型组、加水组小鼠相较于正常组，气道IL-8含量明显升高（P<0.05）;桑杏汤组小鼠气道IL-8含量下降趋势明显（P>0.05）。结论 处于云南春燥环境下的小鼠气道RS含量显著降低、IL-8含量升高明显，且在桑杏汤的干预下均有改善，可初步将RS、IL-8纳入云南春燥动物模型的观测及评价的指标体系之中进一步研究。

关键词：云南春燥;桑杏汤;粘多糖RS;白介素IL-8

中图分类号：R563 文献标志码：A 文章编号：1007-2349（2021）01-0074-04

【Abstract】Objective： To study the effect of spring dry environment on the metabolism of airway body fluid and external health functions in mice by observing the changes of mucopolysaccharide（RS） and interleukin-8（IL-8） contents in airways of the mice in the spring dry environment of Yunnan and explain the traditional Chinese medicine theories such as “dryness brings out deficiency of fluid”， “lung fluid hurts”， “dryness offends lung” and “lung health loses”. Methods： The laboratory mice were replicated to multiple factors of spring dry environment of Yunnan and the disproof model was made by gavage administration of Sangxing Decoction. Results： The airway RS content of the mice in the model group was significantly lower than that of the normal group（P<0.01）. The airway RS content of the mice in the water added group was significantly reduced（P<0.05）. Compared with that of the model group， the content of the airway RS of the mice in the Sangxingtang group significantly increased（P<0.01） and compared with that of the normal group， the airway IL-8 content of mice in the model group and the water added group significantly increased（P<0.05）. The IL-8 content in the airway of the Sangxingtang group had a significant downward trend（P>0.05）. Conclusion： The airway RS content of mice in the spring dry environment of Yunnan was significantly reduced， and IL-8 content was significantly increased， and the content of the two groups improved under the intervention of Sangxing Decoction. RS and IL-8 can be initially included in the indicator system of animal model observation and evaluation.in the spring dry environment for further study.

【Key words】spring dryness in Yunnan， Sangxing Decoction， mucopolysaccharide RS， interleukin IL-8

外燥证，是一为外邪燥气侵袭，以目口鼻咽干涩、干咳少痰、皮肤毛发枯槁、便干结尿少等以津液匮乏之类特征为主要表现的症候[1]。云南因其独特的地势特点，形成夏秋多雨、冬春干燥的气候，加之春季风力强劲及居民擅食辛辣厚味的特点，内外合邪，形成云南特有的春燥证[2-3]。

当前，“云南春燥”相关实验研究已初见成效，本实验拟在前期实验的基础上，将小鼠置于复制云南春季“干燥多风”的气候特点的气候箱中，同时饲与辛辣食料，并予以方药反证，通过观测小鼠表征、组织及体液分子指标的变化，深入探究春燥环境对小鼠气道津液代谢及卫外功能的影响，为建立云南春燥动物模型提供进一步的科学、客观依据。研究内容如下：