张梦师 刘美珍 杨斌

【摘 要】 目的：預测木棉花治疗肝损伤的主要成分和作用靶点，探讨木棉花多成分-多靶点-多通路的作用机制。方法：采用网络药理学方法，依托中药系统药理学分析平台（TCMSP）检索和筛选出木棉花的主要活性成分，借助superpred webserwer和SwissTargetPrediction数据库预测主要活性成分的作用靶点，构建化合物-靶点网络图;通过OMIM 数据库预测出与肝损伤相关的靶点，与木棉花药物预测靶点相映射构建药物-疾病-靶点相互作用网络图;对木棉花与肝损伤相互作用靶点进行GO和KEGG富集分析。结果：TCMSP数据库筛选出木棉花主要活性成分10个，PharmMapper数据库检索到个木棉花验证靶点330个、预测靶点150个，swiss target prediction数据库检索到木棉花潜在靶点319个。木棉花治疗肝损伤的预测靶点为PTGS2、CYP2E1、GSR等20个，GO分析筛选了Heterocycle metabolic process，response to oxidative stress等前10条生物过程。KEGG分析筛选了pentose and glucuronate interconversions，serotonergic synapse，metabolic pathyways等前10条通路在肝损伤方面具有作用。结论：木棉花主要通过氧化应激、氧化还原过程、代谢过程等个生物过程，以及花生四烯酸代谢、ABC转运蛋白、相互转化等信号通路来发挥保肝作用，体现了中药多成分、多靶点、多途径的作用特点。

【关键词】 木棉花;肝损伤;网络药理学

【中图分类号】R285   【文献标志码】 A    【文章编号】1007-8517（2020）20-0019-07

Study on the Mechanism of Gossampiniflos on Liver Injury Based on Network Pharmacology

ZHANG Mengshi LIU Meizhen YANG Bin*

Guangxi Medical University，School of Pharmacy，Nanning 530021，China

Abstract：Objective To predict the active components and targets of Gossampiniflos in the treatment of liver injury， and to explore the mechanism. Methods The effective components of Gossampiniflos were screened by Traditional Chinese Medicine Systems Pharmacology（TCMSP）. The superpred webserwer and SwissTargetPrediction database were used to predict the target of the active components and to construct a compound-target network. The OMIM database was used to predict targets associated with liver injury， compared target of disease with targets of drug to construct drug-disease-target network; performed GO and KEGG analysis on interaction targets.Results The TCMPS database selected 10 major active components. The PharmMapper database retrieved 330 verification targets and 150 predicted targets. The SwissTargetPrediction database retrieved 319 potential targets. The predictive targets for the treatment of liver injury by Gossampiniflos were PTGS2、CYP2E1、GSR， etc. The first 10 biological processes were Heterocycle metabolic process， response to oxidative stress， etc. The first 10 KEGG pathways were pentose and glucuronate interconversions， serotonergic synapse， and metabolic pathways， etc.Conclusion Gossampiniflos plays a significant role in liver protection through oxidative stress， redox process， metabolic process and other biological processes， as well as arachidonic acid metabolism， ABC transporter， and mutual transformation. It reflects the multi-component， multi- target and multi-pathway of Gossampiniflos.

Keywords：Gossampiniflos; Liver Injury; Network Pharmacology

木棉花为木棉科植物木棉Gossampinus malabarica （DC.） Merr.的干燥花，别名斑芝树、英雄花，攀枝花等。其性味：甘、淡，凉;归大肠经;具有清热利湿，解毒的功效[1]。药典记载，由木棉花、广木香、松石等组成的二十五味松石丸，可用于肝郁气滞，肝中毒，以及各种急、慢性肝炎等。伍小燕等[2-3]研究发现木棉花提取物能改善CCl4致大鼠肝纤维，缓解抗结核药物引起的肝损伤。……