闫明雪　王倩倩　杨铭哲　薛文静　史磊　赵盼

摘      要：為了研究核桃楸皮总黄酮的抗氧化活性和体外总还原能力，采用质量分数70%的乙醇回流提取核桃楸皮总黄酮，并用HPD-750型大孔树脂纯化核桃楸皮总黄酮;利用亚硝酸钠-硝酸铝-氢氧化钠显色法测定核桃楸皮总黄酮的质量浓度;通过DPPH自由基清除实验、超氧阴离子自由基清除实验和还原力实验研究核桃楸皮总黄酮的抗氧化活性和体外总还原能力。结果表明：提取物经HPD-750型大孔树脂纯化后所得的总黄酮粉末纯度为（72.02±4.01）%;核桃楸皮总黄酮对DPPH 自由基的半抑制质量浓度IC₅₀为2.068 ?g·mL^-1，对超氧阴离子自由基的半抑制质量浓度IC₅₀为273.253 ?g·mL^-1;还原力实验中，核桃楸皮总黄酮质量浓度为1 mg·mL^-1时吸光度为2.094 4;经统计学分析，核桃楸皮总黄酮的抗氧化活性与总黄酮质量浓度呈显著正相关（P<0.05）。体外抗氧化试验表明核桃楸皮总黄酮具有较好的体外抗氧化活性，以期为食品工业新型抗氧化剂、抗氧化食品以及抗氧化药物的研发提供理论依据。

关  键  词：核桃楸皮;总黄酮;提取纯化;抗氧化

中图分类号：R284       文献标识码： A       文章编号： 1671-0460（2020）06-1051-05

Antioxidant Activity In Vitro of Total Flavonoids From Cortex Juglandis Mandshuricae

YAN Ming-xue， WANG Qian-qian， YANG Ming-zhe， XUE Wen-jing， SHI Lei， ZHAO Pan^*

（College of Pharmacy， Shandong University of Traditional Chinese Medicine， Jinan Shandong 250355， China）

Abstract： To study the antioxidant activity and total reduction capacity in vitro of total flavonoids （TFs） from Cortex Juglandis Mandshuricae （CJM），TFs were extracted from CJM by 70% ethanol and purified by HPD-750 macroporous resin. The system of NaNO₂-Al（NO₃）₃-NaOH was used to determine the content of TFs from CJM. The antioxidant activity and total reduction capacity of TFs from CJM were studied by DPPH free radical scavenging experiments， superoxide anion radical scavenging experiments and reducing power experiments. The results showed that， after the extract was purified by HPD-750 macroporous resin， the content of TFs powder obtained was （72.02±4.01）%. The half inhibitory concentration （IC₅₀） of DPPH free radicals by TFs from CJM was 2.068 ?g·mL^-1， and the half inhibitory concentration （IC₅₀） of superoxide anion radicals was 273.253 ?g·mL^-1. In the reducing power experiment， the absorbance was 2.0944 when the TFs content from CJM was 1 mg·mL^-1. After statistical analysis， there was a significant positive correlation between the antioxidant activity and the content of TFs from CJM （P<0.05）. So TFs from CJM had good antioxidant activity in vitro. The paper can provide theoretical basis for the research and development of new antioxidants for antioxidant foods and antioxidant drugs.

Key words： Cortex Juglandis Mandshuricae; Total flavonoids; Extraction and purification; Antioxidant

核桃楸（Juglandis Mandshuricae）属胡桃科胡桃属植物，主要分布于我国东北、华北地区，是十分珍贵的药源植物，开发应用前景广阔^[1]。核桃楸皮（Cortex Juglandis Mandshuricae）为核桃楸的茎皮和枝皮^[2]，富含黄酮类、醌类、萜类、二芳基庚烷类、多酚类等多种化学成分^[3]，具有抗氧化、抗菌、抗肿瘤等多种生物活性^[4]，在食品、药品等领域都具有较大的开发价值。

黄酮类化合物普遍存在于自然界中，具有抗氧化、抗菌、抗肿瘤、调节免疫等多种药理活性^[5]，但目前针对核桃楸皮的研究多集中在醌类物质及抗肿瘤方面，对核桃楸皮总黄酮抗氧化活性的研究少见报道^[6-7]。……