邹婧　吕燕慧　姚中伟　王悦　张学涛　王玲娜

摘      要：以金銀花为研究对象，采用傅立叶红外光谱技术结合二阶导数红外光谱对不同干燥方法、不同品种的金银花进行分析。检测结果表明：同一品种金银花的不同干燥方法的红外光谱中，在1 047 cm^-1处烤房烘干的“九丰一号”吸收峰强度最高，说明烤房烘干“九丰一号”金银花的黄酮、苷类等物质含量较晒干和杀青烘干的高;金银花红外光谱图中主要吸收峰在3 387、2 913、2 843、1 642、1 380、1 259、1 048、610 cm^-1附近，不同品种金银花的吸收峰基本相似，但吸收峰的位置及强度存在一定差异;同时在金银花与山银花的红外光谱中发现在1 732、1 514、1 459 cm^-1处山银花比金银花吸收明显。因此，红外光谱结合二阶导数光谱可用来反映金银花有效成分、干燥方法和品种的信息。

关  键  词：金银花;山银花;傅立叶红外光谱法;二阶导数红外光谱;鉴别

中图分类号：R282.5; O657.33     文献标识码： A     文章编号： 1671-0460（2020）06-1042-05

Study on Infrared Spectra of Different Varieties of Honeysuckle Dried by Different Methods and Mountain Honeysuckle

ZOU Jing， LV Yan-hui ，YAO Zhong-wei， WANG Yue， ZHANG Xue-tao ，WANG Ling-na^*

（College of Pharmacy， Shandong University of Traditional Chinese Medicine， Experiment Center of Shandong University of Traditional Chinese Medicine， Jinan Shandong 250355， China）

Abstract： Taking honeysuckle as the research object， Fourier transform infrared spectroscopy combined with second derivative infrared spectroscopy was used to analyze different varieties of honeysuckle dried by different methods. The test results showed that，in the infrared spectrum of the same variety of honeysuckle dried by different methods， the intensity of the absorption peak of “Jiufeng No.1” dried in the baking room was the highest at 1 047 cm^-1， indicating that the contents of flavonoids and glycosides in the “Jiufeng No.1” honeysuckle dried in the baking room were higher than those in the “Jiufeng No.1” honeysuckle dried by sun-drying and cyanocyanine drying. The main absorption peaks of honeysuckle infrared spectrum were near 3 387， 2 913， 2 843， 1 642， 1 380， 1 259， 1 048， 610 cm^-1. Absorption peaks of different varieties of honeysuckle were basically similar， but there were some differences in the position and intensity of the absorption peaks. At the same time， in the infrared absorption spectra of honeysuckle and mountain honeysuckle， absorption peaks of mountain honeysuckle were more obvious than those of honeysuckle at   1 732， 1 514， 1 459 cm^-1. Therefore， the infrared spectrum combined with the second derivative spectrum can be used to reflect the information of active ingredients， drying methods and varieties of honeysuckle.

Key words： Honeysuckle; Mountain honeysuckle; Fourier infrared spectroscopy; Second derivative infrared spectroscopy; Identification

金银花的来源为忍冬科植物忍冬的干燥花蕾或带初开的花，山银花的来源为忍冬科植物灰毡毛忍冬、华南忍冬、红腺忍冬或黄褐毛忍冬的干燥花蕾或带初开的花^[1-2]。金银花与山银花均为清热解毒和疏风散热的传统中药，常被人们用来医治疔疮、风热感冒、丹毒、热血痢疾及其他病症^[3-4]。现代药理学研究表明金银花和山银花具有抑菌、杀菌、抗病毒、抗过敏、解热和肝脏保护作用^[5]; 金银花与山银花的化学成分十分复杂， 目前已鉴定出有60多种成分， 其中具有药理活性的主要成分有挥发油、黄酮类化合物、有机酸、三萜皂苷类化合物^[6-8]。……