徐剑 周亚莎 彭俊 潘坤 吴权龙 李建超 刘悦 王英 彭清华

〔摘要〕 目的 探讨枸杞子加丹参对视网膜色素变性模型大鼠RCS（rdy-/-， p-/-）视网膜匀浆中cAMP含量、cGMP含量以及cAMP/cGMP值的影响。方法 实验动物随机分为5组：空白组为RCS（rdy+/+， p+/+）大鼠8只，模型组、枸杞子组、丹参组及枸杞子加丹参组（杞参组）为RCS（rdy-/-， p-/-）大鼠各8只，每组均雌雄各半。5组大鼠分别给予生理盐水、生理盐水、枸杞子、丹参、枸杞子加丹参灌胃。灌胃28 d后，ELISA法测定视网膜组织匀浆中cAMP、cGMP含量及cAMP/cGMP值。结果 与空白组比较，模型组大鼠视网膜匀浆组织中cAMP、cGMP含量及cAMP/cGMP值均增高，差异有显著统计学意义（P<0.01）;与模型组比较，杞参组视网膜匀浆组织中cAMP、cGMP含量及cAMP/cGMP值均明显降低，差异有显著统计学意义（P<0.01）。结论 “补虚活血”代表中药枸杞子加丹参可以降低RCS（rdy-/-，p-/-）大鼠视网膜组织匀浆中cAMP、cGMP含量及cAMP/cGMP值，改善其“虚”证指标。

〔关键词〕 视网膜色素变性;枸杞子;丹参;RCS大鼠;cAMP;cGMP

〔中图分类号〕R276.7       〔文献标志码〕A       〔文章编号〕doi：10.3969/j.issn.1674-070X.2020.07.002

〔Abstract〕 Objective To study the effects of Fructus Lycii and Radix Salviae Miltiorrhizae on the content of cAMP， cGMP and cAMP/cGMP retinal homogenate of RCS （rdy-/-，p-/-） rats. Methods The experimental animals were randomly divided into 5 group. The blank group had 8 RCS （rdy+/+，p+/+） rats. Each of the Fructus Lycii group， the Radix Salviae Miltiorrhizae group， the Fructus Lycii and Radix Salviae Miltiorrhizae （Qishen） group had 8 RCS （rdy-/-， p-/-） rats. Each group had half male and half female rats. The 5 groups were respectively filled the stomach with normal saline， normal saline， Fructus Lycii， Radix Salviae Miltiorrhizae， Fructus Lycii and Radix Salviae Miltiorrhizae by gavage. After 28 days gavage， the content of cAMP， cGMP and cAMP/cGMP in retinal homogenate were determined by ELISA. Results Compared with the blank group， the content of cAMP， cGMP and cAMP/cGMP in the retinal homogenate tissue of the model group were increased， and the difference was statistically significant （P<0.01）; Compared with the model group， the content of cAMP and cGMP and the value of cAMP/cGMP in the retinal homogenate tissue of Qishen group were significantly reduced， and the difference was statistically significant （P<0.01）. Conclusion The representative Chinese materia medica for “tonifying deficiency and activating blood circulation” Fructus Lycii and Radix Salviae Miltiorrhizae can reduce the contents of cAMP， cGMP and cAMP/cGMP in the retinal homogenate tissue of RCS （rdy-/-，p-/-） rats and improve the index of “deficiency”.

〔Keywords〕 retinitis pigmentosa; Fructus Lycii; Radix Salviae Miltiorrhizae; RCS rats; cAMP; cGMP

視网膜色素变性（retinitis pigmentosa， RP）是以夜盲和视野逐渐缩窄为特征的眼底疾病。中医学认为该病主要由先天禀赋不足、肝肾亏虚、脾脏虚弱和血脉瘀滞等导致[1]。上世纪90年代，本研究团队成员经过大量的临床观察和机制研究证实RP患者的病机为本虚标实、虚中夹瘀，且血瘀贯穿本病始终[2]，由此创造性提出以“补虚活血”为基本治则治疗RP患者。

基于团队前期对RP病机及治法的认识[3-8]，我们选用两味具有补虚活血作用的代表性中药——枸杞子和丹参，来研究补虚活血法对RP模型大鼠的影响。枸杞子有滋补肝肾、益精明目之效;丹参可活血调经、祛瘀止痛，常用治各种瘀血病证。两药合用可共奏益肾养肝、活血明目之功。

为了深入地阐明枸杞子加丹参对RP的作用机制，本实验以已验证的“虚中夹瘀”证RP模型……