王献　王海兵

[摘要] 目的 探討长链非编码RNA（lncRNA）尿路上皮癌相关基因1（UCA1）在食管鳞癌中表达及其对癌细胞黏附和迁移影响。

方法 以我院接受手术治疗的52例食管鳞癌病人作为研究对象，取原发灶处部分癌组织及距离原发灶边缘3～5 cm的癌旁组织，检测两者lncRNA UCA1表达水平及其对食管癌细胞侵袭和迁移能力的影响。

结果 PCR检测表明，原发灶癌组织的UCA1表达水平为（1.52±0.56） HU，癌旁组织为（0.95±0.36） HU，两者比较差异有显著性（t=6.174，P<0.01）。低表达UCA1基因能够抑制食管鳞癌细胞Eca109、KYSE170的迁移能力和侵袭能力，过表达UCA1基因能促进食管鳞癌细胞TE1和TE13的迁移能力和侵袭能力，差异均有显著性（F=133.00～1 252.32，P<0.01）。

结论 lncRNA UCA1能够促进食管癌细胞侵袭和迁移，通过影响细胞周期而影响癌细胞的增殖。

[关键词] 食管鳞状细胞癌;RNA，长链非编码;尿路上皮癌相关基因1;肿瘤侵润;肿瘤转移

[中图分类号] R735.1;R342.2

[文献标志码] A

[文章编号] 2096-5532（2020）03-0355-04

doi：10.11712/jms.2096-5532.2020.56.103

[开放科学（资源服务）标识码（OSID）]

[网络出版] http：//kns.cnki.net/kcms/detail/37.1517.R.20200528.0827.002.html;2020-05-28 11：45

EXPRESSION OF THE LONG NON-CODING RNA UROTHELIAL CARCINOMA ASSOCIATED 1 IN ESOPHAGEAL SQUAMOUS CELL CARCINOMA AND ITS EFFECT ON THE ADHESION AND MIGRATION OF CANCER CELLS

WANG Xian， WANG Haibing

（Department of Chest Surgery， Clinical Medical College， Henan University of Science and Technology （The First Aff-

iliated Hospital of Henan University of Science and Technology）， Luoyang 471000， China）

[ABSTRACT]ObjectiveTo investigate the expression of the long non-coding RNA （lncRNA） urothelial carcinoma asso-

ciated 1 （UCA1） in esophageal squamous cell carcinoma and its effect on the adhesion and migration of cancer cells.

MethodsA total of 52 patients with esophageal squamous cell carcinoma who underwent surgical treatment in our hospital were enrolled as subjects. The cancerous tissue of primary lesion and the adjacent tissue 3-5 cm away from the edge of the primary lesion were collected to measure the expression of the lncRNA UCA1 and observe its effect on the invasion and migration abilities of esophageal cancer cells.

ResultsThe results of PCR showed that there was a significant difference in the expression of UCA1 between the tissue of primary lesion and the adjacent tissue （（1.52±0.56） HU vs （0.95±0.36） HU;t=6.174，P<0.01）. Low expression of UCA1 inhibited migration and invasion abilities of esophageal squamous cell carcinoma cells Eca109 and KYSE170， while overexpression of UCA1 promoted the migration and invasion abilities of esophageal squamous cell carcinoma cells TE1 and TE13 （F=133.00-1 252.32，P<0.01）.

ConclusionThe lncRNA UCA1 can promote the invasion and migration of esophageal cancer cells and affect cell proliferation by influencing cell cycle.

[KEY WORDS]esophageal squamous cell carcinoma; RNA， long noncoding; urothelial carcinoma associated 1; neoplasm invasiveness; neoplasm metastasis

食管癌发病是由多种因素共同作用的结果，随着基因高通量测序技术的发展，长链非编码RNA（lncRNA）被认为在多种癌症发展中扮演重要角色[1-2]。已有研究结果表明，lncRNA参与人体细胞增殖、分化、转移、凋亡等生物学活动，其在多种恶性肿瘤发展中也发挥作用[3-6]。尿路上皮癌相关基因1（UCA1）是癌症调节耐药基因，参与多种肿瘤的恶性发展[7-12]，如胃癌、肺癌、直肠癌等。UCA1是一种来源于人类内源性逆转录病毒（HERV-H）家族的lncRNA分子，其基因定位于人类第19号染色体（19p13.12），全长为1.4 kb。lncRNA UCA1最初在膀胱癌被发现，并能够促进肿瘤细胞增殖和转移[13]。目前，lncRNA UCA1在食管癌中表达及其作用研究报道较少。本文对lncRNA UCA1在食管鳞癌中表达及其对癌细胞黏附和迁移的调控进行分析，探讨lncRNA UCA1用于食管鳞癌病人早期诊断及术后监测的临床价值。……