宋晴晴　张慧敏　张烨　张一卉　李景娟　李化银　贺立龙　王凤德　高建伟

摘要：大白菜是我国分布最广、种植面积最大的蔬菜作物，其丰产对安定人民生活起着举足轻重的作用。GID1蛋白是赤霉素信号转导过程中的重要受体，在调控植物生长发育过程中发挥重要作用。本研究采用生物信息学分析方法对大白菜基因组中GID1家族基因进行鉴定，并对该基因结构、染色体分布、系统进化以及表达模式等进行分析。结果表明，大白菜基因组中含有5个GID1家族成员，分别位于4、5、6、7、9号染色体上，各家族成员都只含有1个内含子。系统进化结果显示，单子叶和双子叶植物的GID1蛋白明显处于两个不同分支，大白菜GID1家族成员与拟南芥GID1具有更近的亲缘关系。通过表达模式以及启动子顺式响应元件分析发现，大白菜GID1基因不仅参与生长发育调控还可能参与对不同环境因子的响应过程。

关键词：大白菜;GID1基因;生物信息学分析;表达模式分析

Abstract： Chinese cabbage is the vegetable with the widest distribution and the largest planting area in China. Its yield plays an important role in the stability of the people's life. GID1 protein is an important receptor in the process of gibberellin signal transduction and plays an important role in regulating plant growth and development. In this study， the GID1 family genes in the Chinese cabbage genome were identified by bioinformatics methods， and their gene structure， chromosome distribution， phylogenetics and expression patterns were analyzed. The results showed that the Chinese cabbage genome contained 5 GID1 gene members and was evenly distributed on chromosomes 4， 5， 6， 7 and 9. In addition， members of the Chinese cabbage GID1 family contain only one intron. In phylogenetic evolution， the GID1 family of proteins has distinct branches between dicotyledons and monocotyledons， while the GID1 family members of Chinese cabbage have a closer genetic relationship with Arabidopsis GID1. Through expression pattern and promoter cis-element analysis， it was found that the GID1 gene of Chinese cabbage not only participates in the regulation of growth and development but also may participate in the response process to different environmental factors. This study not only provides clues for us to understand the function of GID1 gene in Chinese cabbage， but also provides an important reference for further application in molecular breeding of Chinese cabbage.

Key words： Chinese cabbage; GID1 gene;Bioinformatics analysis; Expression pattern analysis

大白菜（Brassica rapa L. ssp. pekinensis）起源于中国，常年种植面积2×106 hm2左右，总产约1亿吨，是种植面积、产量最大的蔬菜作物，素有“国菜”之称。长期生产实践表明，大白菜产量与叶球大小直接相关并且受严格的遗传控制。因此，挖掘参与调控大白菜叶球大小的相关基因将不仅有助于阐明叶球发育的分子调控机制，还有助于通过分子辅助育种手段提高大白菜产量。

赤霉素（gibberellin， GA）作为一种重要的植物激素，参与调控植物生长和发育，如种子萌发、植株生长、花器官形成以及果实发育等[1，2]。……