黄玉菊 李冲 陈蕾蕾 谢俊霞

[摘要] 目的 探讨柯诺辛对鱼藤酮所致帕金森病（PD）小鼠运动功能的影响。方法 8周龄雄性C57BL/6n小鼠96只，随机分为4组，每组24只。空白对照组给予空白溶剂，PD模型组给予鱼藤酮（5 mg/kg），雷帕霉素组联合给予雷帕霉素（10 mg/kg）和鱼藤酮（5 mg/kg），柯诺辛组联合给予柯诺辛（10 mg/kg）和鱼藤酮（5 mg/kg），均灌胃给药，每天1次，连续12周。分别于实验周期的第4、8和12周末，应用转棒实验检测各组小鼠在转棒上持续运动的时间。结果 实验周期第4周末和第8周末，空白对照组、PD模型组、雷帕霉素组和柯诺辛组小鼠在转棒上持续运动时间比较差异无显著性（F=1.220、3.638，P>0.05）。第12周末，PD模型组小鼠在转棒上持续运动时间较空白对照组缩短，雷帕霉素组、柯诺辛组在转棒上持续运动时间较PD模型组增加，差异均有统计学意义（F=5.166，P<0.05）;雷帕霉素组与柯诺辛组比较，小鼠持续运动时间差异无统计学意义（P>0.05）。结论 柯诺辛可以改善鱼藤酮所致PD小鼠的运动持久能力。

[关键词] 帕金森病;鱼藤酮;柯诺辛;小鼠;移动

[中图分类号] Q426;R338.2  [文献标志码] A  [文章编号] 2096-5532（2020）02-0153-03

doi：10.11712/jms.2096-5532.2020.56.092 [开放科学（資源服务）标识码（OSID）]

[网络出版] http：//kns.cnki.net/kcms/detail/37.1517.R.20200519.1427.002.html;2020-05-20 08：57

[ABSTRACT] Objective To investigate the effect of corynoxine on the motor function of mice with rotenone-induced Parkinsons disease （PD）.  Methods A total of 96 male C57BL/6n mice， aged 8 weeks， were randomly divided into blank control group， PD model group， rapamycin group， and corynoxine group， with 24 mice in each group. The mice in the blank control group were given blank solvent， those in the PD model group were given rotenone （5 mg/kg）， those in the rapamycin group were given rapamycin （10 mg/kg） and rotenone （5 mg/kg）， and those in the corynoxine group were given corynoxine （10 mg/kg） and rotenone （5 mg/kg）， all by gavage， once a day for 12 consecutive weeks. At the end of weeks 4， 8， and 12 of experiment， the rotarod test was used to measure the duration of continuous movement on a rotating rod.  Results At the end of weeks 4 and 8 of experiment， there was no significant difference in the duration of continuous movement on a rotating rod between the blank control group， the PD model group， the rapamycin group， and the corynoxine group （F=1.220，3.638;P>0.05）. At the end of week 12， the PD model group had a significantly shorter duration of continuous movement on a rotating rod than the blank control group， and compared with the PD model group， the rapamycin group and the corynoxine group had a significant increase in the duration of continuous movement on a rotating rod （F=5.166，P<0.05）. There was no significant difference in the duration of continuous movement on a rotating rod between the rapamycin group and the corynoxine group （P>0.05）.  Conclusion Corynoxine can improve motor duration in mice with rotenone-induced PD.

[KEY WORDS] Parkinsons disease; rotenone; corynoxine; mice; locomotion

帕金森病（PD）是发病率仅次于阿尔茨海默病的第二大神经退行性疾病，其主要临床表现为运动功能障碍。流行病学研究显示，60岁以上人群PD的发病率为1%～2%，并且随着年龄的增加而增加[1-2]。由于PD的病因、病理机制尚未完全阐明，目前仍然缺乏有效的治疗措施。PD病理特征包含路易小体的出现，而路易小体的主要成分是聚集的α-突觸核蛋白。已有研究显示，α-突触核蛋白基因（SNCA）的多倍增加或点突变均可导致α-突触核蛋白错误折叠，从而引发PD[3]。在大……