田新民 石兰英

摘要：为了分析东北林蛙和牛蛙腿部肌肉的基本营养成分，试验分别采用105 ℃恒质法、考马斯亮蓝染色法、凯氏定氮法、索氏提取法、3，5-二硝基水杨酸比色法和马福炉灰化法对东北林蛙和牛蛙腿部肌肉的水分、水溶性蛋白、盐溶性蛋白、总蛋白、粗脂肪、还原糖、总糖和灰分含量进行了比较分析。结果表明，东北林蛙和牛蛙的腿部肌肉水分含量分别为79.21%±0.40%和76.87%±0.76%，水溶性蛋白为2.91%±0.37%和2.56%±0.07%、盐溶性蛋白为6.07%±0.52%和6.44%±0.48%、总蛋白为24.57%±1.86%和25.08%±2.20%、粗脂肪为0.50%±0.05%和0.59%±0.10%、还原糖为0.15%±0.01%和0.33%±0.02%、总糖为2.41%±0.13%和2.34%±0.04%、灰分为4.41%±0.17%和6.63%±0.42%。其中水分、还原糖和灰分含量差异显著（P<0.05），其他成分差异不显著（P>0.05）。

关键词：东北林蛙;牛蛙;肌肉;基本营养成分

中图分类号：Q959.9         文献标识码：A

文章编号：0439-8114（2019）21-0173-03

DOI：10.14088/j.cnki.issn0439-8114.2019.21.038

Abstract： In order to analyze the basic nutritional components of the muscle of Rana dybowskii and Lithobates catesbeiana， and provide reference for food development. The water content， water soluble protein， salt soluble protein， total protein， crude fat， reducing sugar， total sugar and the ash content of leg muscles of Rana dybowskii and Lithobates catesbeiana were determined by 105 ℃ drying constant mass method， coomassie brilliant blue dyeing method， kjeldahl method， soxhlet extraction method， 3，5-dinitrosalicylic acid colorimetric method and volatilization constant mass method. The contents were compared， the results show that the moisture content of leg muscles of Rana dybowskii and Lithobates catesbeiana were 79.21%±0.40% and 76.87%±0.76% respectively， water-soluble protein was 2.91%±0.37% and 2.56%±0.07%， salt-soluble protein was 6.07%±0.52% and 6.44%±0.48%， total protein was 24.57%±1.86% and 25.08%±2.20%， crude fat was 0.50%±0.05% and 0.59%±0.10%， reducing sugar was 0.15%±0.01% and 0.33%±0.02. The total sugar content was 2.41%±0.13% and 2.34%±0.04% respectively. The ash content was 4.41%±0.17% and 6.63%±0.42%. Among them， the contents of water， reducing sugar and ash were significantly different between Rana dybowskii and Lithobates catesbeiana（P<0.05）， but there was no difference in other components （P>0.05）.

Key words： Rana dybowskii; Lithobates catesbeiana; muscle; basic nutritional components

東北林蛙（Rana dybowskii）隶属蛙科林蛙属，主要分布于中国东北林区，是林蛙油的主要来源，同时具有较高的食用价值，其养殖业为东北林区的特色产业[1，2]。牛蛙（Lithobates catesbeiana）隶属蛙科牛蛙属，原产自北美洲，引入中国后已大范围养殖，为食用蛙中的主要种类[3]。东北林蛙和牛蛙作为重要的经济物种，在营养价值方面已进行大量研究，主要围绕总蛋白、粗脂肪、氨基酸、脂肪酸、矿物质元素等方面，并已证明东北林蛙和牛蛙是具有高蛋白、低脂肪、富含多种重要矿质元素的保健性营养食品[4-11]。在食品开发中，水溶性蛋白、盐溶性蛋白、还原糖等重要参数还鲜有报道。因此，本研究对东北林蛙和牛蛙两种重要蛙类腿部肌肉的水分、水溶性蛋白、盐溶性蛋白、总蛋白、粗脂肪、还原糖、总糖和灰分含量进行比较分析，为加快蛙肉的食品开发提供参考。……