张敏娜 王光辉 丁瑞芳 王利涛

摘 要 目的：研究月季花挥发油的最佳提取工艺并对其β-环糊精包合物进行初步研究。方法：采用水蒸气蒸馏法提取月季花中挥发油，以挥发油得率为指标，采用L9（34）正交试验优化最佳提取工艺；采用饱和水溶液-超声法制备挥发油β-环糊精包合物并用显微镜法和红外光谱法对包合物鉴别。结果：挥发油最佳提取工艺条件为A3B1C3，即采用粗粉，加入8倍量的水，浸泡6 h，提取10 h；经显微镜法和红外光谱法分析，月季花挥发油包合前后具有明显不同的特征。结论：本提取工艺简单、可行，可为月季花挥发油新制剂的开发提供科学依据。

关键词 月季花 挥发油 正交试验 β-环糊精

中图分类号：R284.2 文献标志码：A 文章编号：1006-1533（2019）01-0062-04

Extraction of volatile oil from Rosa chinensis Jacq and primary study on the inclusion compound of β-cyclodextrin*

ZHANG Minna**， WANG Guanghui， DING Ruifang， WANG Litao

（Pharmaceutical College， Jining Medical University， Shandong Rizhao 276826， China）

ABSTRACT Objective： To optimize the extraction technology of volatile oil from Rosa chinensis Jacq and to study its inclusion compound of b-cyclodextrin. Methods： Volatile oil was extracted from Rosa chinensis Jacq by steam distillation and its optimal extraction process was selected using the yield of volatile oil as the index by L9（34） orthogonal test. β-cyclodextrin inclusion compound of the volatile oil was prepared by saturated aqueous solution ultrasonic method and identified by microscopy and infrared spectroscopy. Results： The optimal process for volatile oil extraction was as follows： using coarse powder， 8-fold water， soaking for 6 h， extracting for 10 h. The analysis by microscopy and infrared spectroscopy showed that the inclusion of volatile oil from Rosa chinensis Jacq had distinct characteristics before and after inclusion. Conclusion： This technology is simple and feasible， can provide a scientific basis for the development of new preparation of the volatile oil from Rosa chinensis Jacq.

KEy WORDS Rosa chinensis Jacq； volatile oil； orthogonal experiment； β-cyclodextrin

月季花为蔷薇科植物月季（Rosa chinensis Jacq.）的干燥花，全年均可采收，主要含有黄酮类、酚酸类和挥发油等成分[1-3]，具有活血調经，疏肝解郁之功效[4]。近年来，对月季花的研究主要集中在化学成分的提取分离、同科植物鉴别及药理作用研究上[5-7]，对挥发油提取工艺的研究较少。为了有效提取月季花中挥发油，本文采用水蒸气蒸馏法，以挥发油得率为评价指标，考察粉碎度、加水倍数、提取时间和浸泡时间等因素对挥发油得率的影响，通过正交试验优选月季花挥发油最佳提取工艺。由于挥发油易挥发、性质不稳定，在制剂或贮存时易损失，从而影响成品质量，因此，本文采用b-环糊精包合技术，将其制成包合物，为月季花挥发油新制剂的开发提供科学依据。……