王元惠　冯媛媛　韩植芬

摘要：目的 观察补肾健脾方对人结肠癌移植瘤生长及血清血管内皮生长因子（VEGF）和基质金属蛋白酶7（MMP-7）表达的影响，探讨其抗结肠癌的部分作用机制。方法 裸鼠腋下接种人结肠癌细胞，建立人结肠癌裸鼠皮下移植瘤模型。随机分为模型组、5-氟尿嘧啶组和补肾健脾方低、中、高剂量组，各给药组给予相应药物，连续3周。各组处死6只荷瘤鼠，测量瘤体质量，计算相对抑瘤率；ELISA测定血清VEGF及MMP-7表达；剩余荷瘤鼠用于观察生存时间。结果 补肾健脾方各剂量组瘤体质量较模型组均明显降低，荷瘤鼠生存时间较模型组明显延长；与模型组比较，补肾健脾方各剂量组VEGF、MMP-7表达均明显下降。结论 补肾健脾方可抑制人结肠癌裸鼠皮下移植瘤生长、延长荷瘤鼠生存时间，其机制可能与下调 VEGF、MMP-7表达有关。

关键词：补肾健脾方；结肠癌；血管内皮生长因子；基质金属蛋白酶7；BALB/c 裸鼠

DOI：10.3969/j.issn.1005-5304.2018.06.013

中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2018）06-0053-03

Effects of Bushen Jianpi Prescription on Tumor Growth in Subcutaneous Colorectal Cancer Xenografts in Mice and Expressions of VEGF and MMP-7

WANG Yuan-hui1，2， FENG Yuan-yuan2， HAN Zhi-fen2， SUN Xiao-ting2， LIU Xuan2， DENG Wan-li2， LI Qi2

1. Jiangwan Hospital， Hongkou District， Shanghai 200434， China；

2. Shuguang Hospital， Shanghai University of Traditional Chinese Medicine， Shanghai 201203， China

Abstract： Objective To investigate the effects of Bushen Jianpi Prescription on tumor growth in subcutaneous colorectal cancer xenografts in mice and expressions of VEGF and MMP-7； To discuss its mechanism of anti-colon cancer. Methods Nude mice were inoculated subcutaneously into human colon cancer cells to establish human colon cancer xenograft models. Nude mouse models of subcutaneous colorectal cancer xenografts were randomly divided into model group， 5-FU group， Bushen Jianpi low-， medium-， and high-dose groups. Each medication group was given relevant medicine for three weeks. Six tumor-bearing mice in each group were sacrificed. Tumor mass was measured， and the relative inhibition rate was calculated. The serum levels of VEGF and MMP-7 were measured by ELISA. The remaining tumor-bearing mice were used to observe the survival time. Results Compared with model group， the weight of tumor were reduced and the median survival time of mice were prolonged in Bushen Jianpi groups， as well as the expression levels of VEGF and MMP-7 significantly decreased Bushen Jianpi groups. Conclusion Bushen Jianpi Prescription can inhibit the growth of human colon cancer xenografts in nude mice and prolong the survival time of tumor-bearing mice， which may be related to the down-regulation of the expressions of VEGF and MMP-7.

Keywords： Bushen Jianpi Prescription； colorectal cancer； VEGF； MMP-7； BALB/c nude mice

大腸癌目前治疗方法以手术切除、放化疗为主，但仍有高复发率、高转移率及高病死率的特点。中医药在防治肿瘤复发和转移有一定的疗效[1-2]。复方补肾健脾方是上海中医药大学附属曙光医院肿瘤科临床经验方，有益气补肾健脾、解毒消肿散结之功效，临床疗效较好[3]，但其机制尚不明确。本实验建立人结肠癌裸鼠皮下移植瘤模型，采用补肾健脾方进行干预，观察其对人结肠癌裸鼠移植瘤的作用，探讨其抗结肠癌的部分机制，为其临床应用提供依据。……