符超君　凌艳君　颜家朝

摘要：目的 观察双丹明目胶囊对糖尿病视网膜病变（DR）大鼠视网膜组织低氧诱导因子-1α（HIF-1α）、核因子-κB（NF-κB）表达的影响，探讨其可能的作用机制。方法 SD大鼠尾静脉一次性注射STZ（50 mg/kg）诱导糖尿病模型，眼底荧光血管造影确认DR模型成功。随机分为模型组、中药复方组和阳性药组，同时以正常大鼠为对照。末次给药后，检测大鼠血糖，观察视网膜病变，Western blot和RT-PCR分别检测大鼠视网膜HIF-1α和NF-κB蛋白和基因的表达。结果 与对照组比较，模型组大鼠血糖显著升高，视网膜微血管增加、荧光素渗漏明显，HIF-1α、NF-κB蛋白和基因表达均升高；与模型组比较，中药复方组大鼠血糖、视网膜微血管数量下降，荧光素渗漏面积减少，HIF-1α、NF-κB蛋白和基因表达均降低。结论 双丹明目胶囊可有效抑制DR大鼠新血管生成，保护视网膜组织，其作用可能与下调视网膜HIF-1α、NF-κB的表达有关。

关键词：糖尿病视网膜病变；双丹明目胶囊；视网膜；低氧诱导因子-1α；核因子-κB；大鼠

DOI：10.3969/j.issn.1005-5304.2018.06.011

中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2018）06-0044-04

Effects of Shuangdan Mingmu Capsules on Expressions of HIF-1α and NF-κB

in Retina of Diabetic Retinopathy Rats

FU Chao-jun1， LING Yan-jun2， YAN Jia-zhao1， ZHAO Hong-qing1， LV Yan1， HE Wen-long1， QIN Yu-hui1

1. Hunan University of Chinese Medicine， Changsha 410007， China；

2. Hunan Academy of Chinese Medicine， Changsha 410006， China

Abstract： Objective To observe the effects of Shuangdan Mingmu Capsules on the expressions of HIF-1α and NF-κB； To explore its possible mechanism of treatment. Methods SD rats were injected with STZ （50 mg/kg） into the tail vein to induce diabetes mellitus. After the DR model was confirmed by fundus fluorescein angiography， the rats were randomly divided into model group， Shuangdan Mingmu Capsules group and positive medicine group， and normal rats were set as control group. After the last administration， the blood glucose level and retinopathy in rats were measured. The protein and gene expression of HIF-1α and NF-κB in the retina were detected by Western blot and RT-PCR respectively. Results Compared with control group， the blood glucose in the model group was significantly increased； the retinal capillaries increased； the leakage of fluorescein was obvious； the protein and gene expressions of HIF-1α， NF-κB were significantly increased. After administration of Shuangdan Mingmu Capsules， the model rats decreased blood glucose； retinal microvascular volume decreased； fluorescein leakage area was significantly reduced； protein and gene expressions of HIF-1α and NF-κB were decreased. Conclusion Shuangdan Mingmu Capsules can effectively inhibit the neovascularization of DR rats and protect the retina， which may be related to the down-regulation of the expressions of HIF-1α and NF-κB.

Keywords： diabetic retinopathy； Shuangdan Mingmu Capsules； retina； HIF-1α； NF-κB； rats

糖尿病视网膜病变（diabetic retinopathy，DR）是糖尿病最严重的微血管并发症之一，发病率高且危害性大，是患者视力下降甚至失明的主要原因。据报道，约20%～40%糖尿病患者有不同程度的视网膜病变情况，且DR的发生率随糖尿病病程的發展而明显升高[1]。目前，DR的治疗尚缺乏有效药物，较公认的治疗药物是羟苯磺酸钙胶囊，但其依赖性高、不良反应较大。双丹明目胶囊主治肝肾阴虚、瘀血阻络所致的DR，临床应用疗效较好[2-3]。本课题组前期研究表……