冯琪　尚华杰　刘美琪

摘要：目的 观察电针对血管性认知障碍（VCI）大鼠学习记忆能力和大脑皮层炎症因子的影响，探讨针刺防治VCI的作用机制。方法 采用颈内动脉微栓子体外注入法制备VCI大鼠模型。将成模大鼠随机分为模型组、阳性药组和电针组，同时取正常大鼠作为对照组。造模3 d后，阳性药组给予盐酸多奈哌齐药液灌胃，电针组针刺“百会”“足三里”。治疗结束后，采用Morris水迷宫检测大鼠学习记忆能力，ELISA检测大鼠脑组织肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）-6、IL-1β的含量。结果 定位巡航实验随训练次数的增加，阳性药组和电针组大鼠寻找平台平均逃避潜伏期较模型组均有不同程度的缩短；空间探索实验阳性药组和电针组较模型组首次穿越平台所需时间显著缩短；与对照组比较，模型组TNF-α、IL-6、IL-1β含量显著升高；与模型组比较，阳性药组和电针组TNF-α、IL-6、IL-1β含量均显著降低。结论 电针“百会”“足三里”可能通过降低VCI大鼠大脑皮层TNF-α、IL-6和IL-1β的含量，改善大鼠學习记忆能力。

关键词：血管性认知障碍；电针；百会；足三里；炎症因子；学习记忆能力；大鼠

DOI：10.3969/j.issn.1005-5304.2018.06.010

中图分类号：R245 文献标识码：A 文章编号：1005-5304（2018）06-0039-05

Effects of Electroacupuncture on Learning and Memory Ability and Brain Inflammatory Factor in Rats with Vascular Cognitive Impairment

FENG Qi， SHANG Hua-jie， LIU Mei-qi， LIN Zhao-yan， QIU Ling-hui， ZANG Ying-ying，

CHEN Xin-li， LIU Yue-ting， CHEN Hui-yun， WANG Zhao-yang

School of Acupuncture and Moxibustion， Beijing University of Chinese Medicine， Beijing 100029， China

Abstract： Objective To observe the effects of electroacupuncture on the learning and memory ability and cerebral cortex inflammatory factor of rats with vascular cognitive impairment （VCI）； To discuss the mechanism of electroacupuncture for preventing and treating VCI. Methods VCI rat models were made in microemboli injection through internal carotid artery method. The successful modeled rats were randomly divided into model group， positive medicine group and electroacupuncture group， and normal rats were taken as control group. Three days after rat models were established， the positive medicine group was given donepezil hydrochlorideby gavage， and electroacupuncture group was given electroacupuncture at “Baihui” and “Zusanli” acupoints. After treatment， the learning and memory ability was detected by Morris water maze test. The contents of TNF-α， IL-6 and IL-1β in rat brain tissue were detected by ELISA. Results The water maze results showed that with the increase of the number of training， the average escape latency of rats to find platform in positive medicine group and electroacupuncture group all had different degrees of shortening in positioning cruise experiment； in space exploration experiment， positive medicine group and electroacupuncture group to cross the platform area for the first time were significantly reduced compared with the model group； compared with the control group， the contents of TNF-α， IL-6 and IL-1β in the model group were increased significantly； compared with the model group， the contents of TNF-α， IL-6 and IL-β in

基金项目：国家自然科学基金重点项目（81430100）

通讯作者：王朝阳，E-mail：xlywzy@163.com

postive medicine group and electroacupuncture group were decreased. Conclusion Electroacupuncture at “Baihui” and “Zusanli” acupoints can decrease the contents of TNF-α， IL-1 and IL-6 in the cortex of VCI rats， and improve the learning and memory ability of rats.

Keywords： vascular cognitive impairment； electroacupuncture； Baihui； Zusanli； inflammatory factor； learning and memory ability； rats

腔隙性腦梗死是大脑或脑干深部的小穿通动脉管壁发生病变，最终管腔闭塞导致的缺血性微梗死，是脑梗死的常见亚型之一。血管性认知功能障碍（vascular cognitive impairment，VCI）是由所有脑血管危险因素、明显或不明显的脑血管病引起的从轻度认知障碍到痴呆的一大类综合征。……