杨姣　贺福元　刘惠　张雨恬　周逸群　肖美凤　杨岩涛　周晋

摘要：目的 利用已建立的中药材成分封闭体系提取动力学模型，探讨金银花与山银花绿原酸的提取动力学差异。方法 采用HPLC测定金银花与山银花绿原酸有效含量W0及分配系数ρ2，采用吸水法测定工艺参数V1、V2再计算V0，利用SPSS19.0软件非线性拟合得到参数M、N、L、α、β、π，用Excel2016按公式计算k、k1′、k2′、ρ1、tmax、cmax、AUC、P%、D%。运用总量统计矩相似度法计算两者的相似度。结果 金银花的V0、V1、V2分别为18.69、9.50、30.20 mL，金銀花绿原酸W0为3.75%、ρ2为0.884；山银花的V0、V1、V2分别为12.79、7.80、37.00 mL，山银花绿原酸W0为5.67%、ρ2为1.020。金银花与山银花绿原酸药时曲线呈三室模型，其主要提取动力学参数k分别为0.110 1、0.3755 h-1，k1′分别为3.632、3.288 h-1，k2′分别为53.12、55.28 h-1，ρ1分别为2.731、2.751，tmax分别为0.299 5、0.216 3 h，cmax分别为0.134 0、0.252 7 mg/mL，AUC分别为3.405、1.560 h，P%分别为35.73%、44.57%，D%分别为0.916 2%、2.680 7%。总量统计矩相似度为0.963 8，表明金银花与山银花绿原酸提取动力学行为基本相似。结论 金银花与山银花绿原酸的溶出行为及其差异可以用中药提取动力学数学模型客观且有效地表达，本研究可以为进一步开展金银花与山银花提取工艺的优化及其制剂研究提供一定参考。

关键词：金银花；山银花；绿原酸；提取动力学模型；封闭体系；总量统计矩相似度

DOI：10.3969/j.issn.1005-5304.2018.04.019

中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2018）04-0091-05

Abstract： Objective To explore the extraction kinetic deviation of the chlorogenic acid （ChA） in Lonicerae Japonicae Flos and Lonicerae Flos through established extract kinetic model of closed system of Chinese materia medica. Methods The content of ChA （W0） and ρ2 in Lonicerae Japonicae Flos and Lonicerae Flos were determined by HPLC； The V1 and V2 were measured by water absorbing further to calculate V0； The value of M， N， L， α， β， and π were estimated by curve fitting using SPSS19.0 edition； The extraction kinetic parameters k， k1′， k2′， ρ1， tmax， cmax， AUC， P%， and D% were calculated by Excel； their similarity was calculate by the total quantum statistical moment similarity （TQSMS）. Results The V0， V1， and V2 for Lonicerae Japonicae Flos were 18.69， 9.50， and 30.20 mL， respectively. The W0 for the ChA in Lonicerae Japonicae Flos were 3.75%， and ρ2 was 0.884； The V0， V1， and V2 for Lonicerae Flos were 12.79， 7.80， and 37.00 mL， respectively. The W0 for the ChA in Lonicerae Flos were 5.67%， and ρ2 was 1.020； The extraction kinetic profiles for ChA in Lonicerae Japonicae Flos and Lonicerae Flos were fitted three compartment model. The main kinetic parameters as k were 0.1101， 0.3755 h-1； k1′ were 3.632， 3.288 h-1； k2′ were 53.12， 55.28 h-1； ρ1 were 2.731， 2.751； tmax were 0.299 5， 0.216 3 h； cmax were 0.134 0， 0.252 7 mg/mL； AUC were 3.405， 1.560 h； P% were 35.73% and 44.57%； D% were 0.916 2% and 2.680 7%， respectively. Their TQSMS was 0.963 8， which indicated that the extraction kinetics of ChA in Lonicerae Japonicae Flos and Lonicerae Flos had good similarity. Conclusion The extraction kinetic model described the dissolution behavior and deviation of extraction kinetic profiles for ChA in Lonicerae Japonicae Flos and Lonicerae Flos objectively and effectively. This research can provide some references for further study on extraction processes and preparation of Lonicerae Japonicae Flos and Lonicerae Flos.

Keywords： Lonicerae Japonicae Flos； Lonicerae Flos； chlorogenic acid； extraction kinetic model； closed system； total quantum statistical moment similarity

中药材由于生物体组织构成及成分的复杂性和多样性，影响其提取因素众多，但其综合效果可用提取动力学数学模型及参数表达。中药成分从药材组织中溶出经历3个阶段[1-2]：首先，溶剂向药材内部渗透和药材的润湿，即浸润解吸；其次，依靠溶质的溶剂化等将溶质溶解到固液界面上，即置换溶解；……