李佳佳 郑文丽 张华潭 李春花

[摘要] 目的 研究葶藶生脉方中黄芪、麦冬皂苷的含量测定方法，并以其含量为指标优选黄芪、麦冬的最佳提取工艺。 方法 选用以香草醛-冰醋酸-高氯酸为显色剂，紫外分光光度法测定总皂苷的含量。采用正交试验法，以总皂苷的含量为评价指标，考察乙醇浓度、料液比和提取时间对提取工艺的影响，优选黄芪、麦冬的最佳提取工艺。 结果 本研究中黄芪甲苷在8.88～44.40 μg范围内呈良好的线性关系，精密度、稳定性、重现性试验的RSD值均≤1.19%，平均加样回收率为101.78%，RSD值为1.85%（n = 9）。最佳提取工艺：8倍量80%的乙醇回流提取2次，每次2 h。 结论 该方法操作简便，准确、重现性好，可作为葶苈生脉方中黄芪、麦冬皂苷质量控制的方法。

[关键词] 黄芪；麦冬；总皂苷；葶苈生脉方；提取工艺；正交试验

[中图分类号] R284.2 [文献标识码] A [文章编号] 1673-7210（2018）02（c）-0128-04

Extraction and content determination of total saponins from Astragali Radix and Ophiopogonis Radix in Tingli Shengmai Decoction

LI Jiajia1 ZHENG Wenli2 ZHANG Huatan3 LI Chunhua4

1.Grduate School， Hebei University of Chinese Medicine， Hebei Province， Shijiazhuang 050090， China； 2.Department of Pharmacy， Shijiazhuang Second Hospital， Hebei Province， Shijiazhuang 050051， China； 3.Shijiazhuang Keren Pharmaceutical Technology Co.， Ltd， Hebei Province， Shijiazhuang 050035， China； 4.College of Pharmacy， Hebei University of Chinese Medicine， Hebei Province， Shijiazhuang 050200， China

[Abstract] Objective To study the content determination method of total saponins from Astragali Radix and Ophiopogonis Radix in Tingli Shengmai Decoction， and to optimize the optimum extraction process of Astragali Radix and Ophiopogonis Radix taking the contents as indices. Methods The vanillic aldehyde-glacial acetic acid-perchloric acid was selected as color developing agent， and the contents of total saponins were detected by ultraviolet spectrophotometry. By using orthogonal experiment， taking the contents of total saponins as indices， the effects of ethanol concentration， solid-liquid ratio and extraction duration for the extraction process were investigated， so as to optimize the optimum extraction process of Astragali Radix and Ophiopogonis Radix. Results In this study， astragaloside Ⅳ showed a good linear relationship within the range of 8.88-44.40 μg， all the RSD values of degree of precision， stability and repeatability experiments were ≤1.19%， the average recovery rate was 101.78%， RSD was 1.85% （n = 9）. The optimum extraction process： reflux extraction of 8 times amount of 80% ethanol was taken for 2 times， once 2 h. Conclusion The method is simple， accurate and reproducible， which can be used as a method for the quality control of Astragali Radix and Ophiopogonis Radix in Tingli Shengmai Decoction

[Key words] Astragali Radix； Ophiopogonis Radix； Total saponins； Tingli Shengmai Decoction； Extraction process； Orthogonal experiment

葶苈生脉方是全国名老中医邢月朋治疗气虚血瘀、阳虚水停型充血性心力衰竭（CHF）的经验方，该方是由葶苈子、黄芪、麦冬、红参、五味子、桂枝、茯苓、白术、猪苓、泽泻、丹参、红花、川芎组成，被列为国家“十一五”科技支撑计划项目。为将葶苈生脉方开发为现代制剂，我们探索适于方中黄芪、麦冬工业生产的工艺参数。方中黄芪、麦冬含有皂苷类成分，且均为有效成分。现代药理研究已证实：黄芪皂苷有强心作用，尤以黄芪甲苷具有明显的正性肌力作用，可以改善心脏收缩和舒张功能[1-4]；麦冬皂苷对心肌细胞缺氧再给氧损伤具有保护作用[5-6]。文献中多为黄芪、麦冬单味药材的提取研究[7-10]，本研究将对黄芪、麦冬药对进行提取工艺的优化，为实际生产提供科学依据。……