白婧

【摘 要】目的：比较不同采样方法在检测高校公共设施表面菌落总数的差异，从而寻找更适合于检测不同表面质地的公共设施的采样方法；方法：在某高校中随机选取教室和宿舍门把手、餐厅内餐桌椅、图书馆图书等公共设施和物品的表面， 分别应用涂抹法和贴斑法采集并检测相同物体表面菌落总数，比较不同采样方法的差异性；结果：对门把手等光滑物体表面菌落总数检测分析，得出两种采样方法差异不显著（P>0.05）；而对图书馆图书等非光滑物体表面的检测，得出两种采样方法差异显著（P<0.05），贴斑法的檢测结果明显高于涂抹法；结论 不同表面质地的物体表面需要用不同的采样方法，其中，非光滑物体表面需要采用贴斑法进行采样，才能够保证检测结果的准确性。

【关键词】采样方法；高校公共设施；细菌污染

中图分类号： R187 文献标识码： A 文章编号： 2095-2457（2018）04-0027-002

A Comparative Study of Bacterial Contamination on the Surface of Public Facilities in a University was

Examined by Different Sampling Methods

BAI Jing

（Shandong union college，jinan shandong 250109，China）

【Abstract】Objective to compare the differences in the total number of bacterial colonies on the surface of public facilities in colleges and universities in order to find out the sampling method of public facilities more suitable for testing different surface textures；Method randomly selected in a university classroom and dormitory door handles，the eat desk and chair inside dining-room，library books and other public facilities and the surface of objects，respectively applied daub and post spot method to collect and detect the same surface total number of colonies，comparing the differences of different sampling methods；Results there was no significant difference between the two sampling methods（P BBB 0.05）.On the surface of non-smooth surfaces such as library books，the difference between the two sampling methods is significant （P <0.05），and the detection result of the patch method is significantly higher than that of the daub method.Conclusion the surface of different surface texture needs different sampling methods，among which，the surface of non-smooth surface needs to be sampled by spot method，so as to ensure the accuracy of test results.

【Key words】Sampling method；Public facilities in colleges and universities；Bacterial contamination

高校公共设施和公共用品表面的微生物污染情况反映了高校的卫生状况，也是监测其消毒措施及消毒效果优劣的主要依据。评价物体表面微生物污染情况在各种医疗卫生领域有着广泛应用，采样方法的优劣直接影响到检测结果[1]。物体表面微生物污染检测的采样方法通常分为涂抹法和斑贴法两种。有关学校公共设施对这两种采样方法比较研究尚不多见，本研究尝试采用贴斑法和涂抹法对待检样品进行采样，分析不同采样方法对公共设施表面检测结果的影响，旨在为正确选择采样方法，提高检测水平提供依据。

1 材料与方法

1.1 采集样品来源

采样物品包括光滑物品表面和非光滑物品表面，其中光滑物品表面选取了教室、宿舍和卫生间门把手；非……