宋漫玲 陶俊宇 杨剑 胡庭俊

摘要：目的 采用大孔吸附树脂对辣蓼总黄酮进行分离纯化，探讨静态及动态吸附过程中多种因素对吸附及解吸效果的影响，以确定其最佳分离提纯工艺。方法 提取辣蓼总黄酮，测定辣蓼黄酮乙酸乙酯部位（FEA）与辣蓼黄酮正丁醇部位（FNB）黄酮含量，进行大孔吸附树脂对FEA与FNB的吸附试验，采用D101、AB-8、DM130和XDA-8大孔树脂优选最佳分离纯化工艺。结果 选择XDA-8进行辣蓼总黄酮的富集，其最佳工艺为：调节FEA黄酮pH值为6，上样浓度为750 μg/mL，用75%乙醇洗脱，以1 BV/h流速洗脱5 BV；调节FNB黄酮pH值为6.0，上样浓度为1 mg/mL，用60%乙醇洗脱，以1 BV/h流速洗脱5 BV。结论 采用XDA-8大孔吸附树脂通过最佳工艺有利于分离纯化辣蓼总黄酮。

关键词：大孔吸附树脂；辣蓼总黄酮；分离纯化

DOI：10.3969/j.issn.1005-5304.2018.01.016

中图分类号：R284.2 文献标识码：A 文章编号：1005-5304（2018）01-0074-05

Study on Separation and Purification of Total Flavonoids from

Polygonum hydropiper Linn. by Macroporous Resin

SONG Man-ling1， TAO Jun-yu2， YANG Jian1， HU TING-jun1

1. School of Animal Science and Technology， Guangxi University， Nanning 530005， China；

2. School of Preclinical Medicine， Guangxi Medical University， Nanning 530021， China

Abstract： Objective To study the separation and purification of total flavonoids from Polygonum hydropiper Linn. by macroporous adsorption resin； To investigate the effects of various factors in the process of static and dynamic adsorption on effects of adsorption and desorption to determine the optimum capability. Methods The total flavonoids from Polygonum hydropiper Linn. was extracted； the contents of flavonoids of ethyl acetate part （FEA） and flavonoids of n-butanol part （FNB） were detected； the adsorption experiment of FEA and FNB was conducted by macroporous adsorption resin. D101， AB-8， DM130， and XDA-8 macroporous resin were used to optimize the separation and purification process. Results The XDA-8 macroporous resin was selected to enrich total flavonoids from Polygonum hydropiper Linn. The optimum process was： adjusting the pH value of FEA flavonoids to 6， the concentration of sample was 750 g/mL， eluting with 75% ethanol to elute 5 bed volumes at a flow rate of 1 bed per hour； adjusting the pH value of FNB flavonoids to 6， the concentration of sample was 1 mg/mL， eluting with 60% ethanol to elute 5 bed volumes at a flow rate of 1 bed per hour. Conclusion XDA-8 macroporous adsorption resin is helpful to separate and purify the total flavonoids of Polygonum hydropiper Linn. by the best process.

Keywords： macroporous adsorption resin； Polygonum hydropiper Linn.； total flavonoids； separation and purification

辣蓼為蓼科蓼属植物水蓼Polygonum hydropiper L.的全草，其主要成分为鞣质、黄酮和挥发油，其中黄酮类化合物有芦丁、槲皮素、金丝桃苷、山柰酚、异鼠李素[1]。近年研究表明，黄酮类化合物具有良好的抗菌[2]、抗病毒[3]、抗氧化[4]生理活性，还能降血压[5]、

基金项目：国家自然科学基金（31560708）

通讯作者：胡庭俊，E-mail：tingjunhu@126.com.

降血脂[6]和提高机体免疫力[7]。大孔吸附树脂是一类有机高分子聚合物吸附剂，近年来被广泛运用于中草药有效成分的分离及新药的开发与研制，其具有价格便宜、稳定性高、吸附快、吸附容量大、洗脱率高、解吸条件温和、再生简便、使用周期长等优点。本试验应用大孔吸附树脂对辣蓼总黄酮进行分离纯化，探讨静态及动态吸附过程中多种因素对吸附及解吸效果的影响，为开发利用辣蓼提供依据。……