贲定严 宁炯杰 徐寅 罗燕 李木清 王云辉 易展?

摘要：目的 观察不同灸量隔姜灸对脾虚证大鼠胃组织丝裂原细胞外激酶（MEK）1/2和细胞外调节蛋白激酶（ERK）1/2表达的影响，探讨隔姜灸治疗脾虚证的可能作用机制及量效特征。方法 将75只SD大鼠按随机数字表法分为空白对照组、模型组、隔姜灸3壮组、隔姜灸6壮组、隔姜灸9壮组，每组15只。200%大黄浓缩液4 ℃灌胃制作脾虚证大鼠模型。造模成功后，隔姜灸组选取“足三里”“中脘”予不同灸量治疗，连续8 d。HE染色镜下观察大鼠胃组织病理学改变，免疫组化检测大鼠胃组织MEK1/2及ERK1/2的蛋白表达。结果 与空白对照组比较，模型组大鼠胃黏膜损伤明显，可见较大的破损、脱落；与模型组比较，隔姜灸3壮组大鼠胃黏膜表面有部分脱落、破损情况改善，隔姜灸6壮组和隔姜灸9壮组大鼠胃黏膜表面较完整、脱落及破损明显改善。与空白对照组比较，模型组胃组织MEK1/2及ERK1/2蛋白表达明显升高（P<0.01）；与模型组比较，隔姜灸各组胃组织MEK1/2及ERK1/2蛋白明显表达升高（P<0.01）；与隔姜灸3壮组比较，隔姜灸6壮组和隔姜灸9壮组胃组织MEK1/2及ERK1/2蛋白表达明显升高（P<0.01），但二者作用效果相当，差异无统计学意义（P>0.05）。结论 隔姜灸通过提高胃组织MEK1/2及ERK1/2的蛋白表达，激活MEK/ERK信号转导通路，进而促进脾虚证大鼠胃黏膜的修复。

关键词：灸量；隔姜灸；脾虚证；胃黏膜增殖修复；丝裂原细胞外激酶；细胞外調节蛋白激酶；大鼠

DOI：10.3969/j.issn.1005-5304.2018.01.012

中图分类号：R245 文献标识码：A 文章编号：1005-5304（2018）01-0054-05

Effects of Different Dosages of Moxibustion with Ginger-Separated Moxibustion on MEK1/2 and ERK1/2 of Gastric Tissue in Rats with Spleen Deficiency

BI Ding-yan1， NING Jiong-jie1， XU Yin2， LUO Yan3， LI Mu-qing1， WANG Yun-hui1， YI Zhan1

1. Hunan University of Chinese Medicine， Changsha 410208， China；

2. The First Hospital of Hunan University of Chinese Medicine， Changsha 410007， China；

3. Hunan Academy of Chinese Medicine， Changsha 410006， China

Abstract： Objective To observe effects of different dosages of moxibustion with ginger-separated moxibustion on expressions of mitogen extracellular kinase （MEK） 1/2 and extracellular regulated protein kinase （ERK） 1/2 of gastric tissue in rats with spleen deficiency； To explore the possible mechanism and the dose-effect relationship. Methods Seventy-five SD rats were randomly divided into blank control group， model group， ginger-separated moxibustion for three zhuang group， six zhuang group and nine zhuang group according to random digits table method， with fifteen rats in each group. The rat model of spleen deficiency was established by intragastric administration with 200% Rhei Radix et Rhizoma infusion at 4 ℃. Ginger-separated moxibustion groups were treated with different dosage of moxibustion at “Zusanli”， “Zhongwan” for eight days after the modeling. Pathological changes of gastric tissue by HE staining were observed under light microscope， and immunohistochemistry was used to detect the expressions of MEK1/2 and ERK1/2 protein in gastric tissue of rats. Results Compared with the blank

基金项目：湖南省教育厅科研计划项目（13C685）；湖南省研究生科研创新项目（CX2016B351）

通讯作者：易展，E-mail：2271813059@qq.com

control group rats， the gastric mucosa injury in the model group was obvious， which showed that the damage and abscission was more serious； compared with the model group， the gastric mucosa of rats was partly exfoliated and the damage was improved in three zhuang group， and the surface of gastric mucosa of rats was more complete and damage was improved obviously in six zhuang group and nine zhuang group； compared with the blank control group， the expressions of MEK1/2 and ERK1/2 protein in gastric tissue increased obviously in other groups （P<0.01）； compared with three zhuang group， the expressions of MEK1/2 and ERK1/2 protein in gastric tissue increased in six zhuang group and nine zhuang group （P<0.01）， but the effects of the two group were similar， without statistical significance （P>0.05）. Conclusion Ginger-separated moxibustion can repair gastric mucosa in rats with spleen deficiency， which may be closely associated with its effect in increasing the expressions of MEK1/2 and ERK1/2 protein in gastric tissue and activating the MEK/ERK signal transduction pathway.

Keywords： dosage of moxibustion； ginger-separated moxibustion； spleen deficiency； proliferation and restoring of gastric mucosa； mitogen activated protein kinase； extracellular regulated protein kinase； rats

脾虚证是指脾气虚弱导致脾失运化的病证，是消化性溃疡、慢性胃炎等消化系统疾病的常见中医证型。有研究发现，脾虚大鼠胃底部黏膜缺损、炎细胞浸润、血管扩张充血、黏膜下层水肿[1]。……