严国鸿 张玉琴 林雄 黄燕 翁淑琴 徐伟

摘要：目的 观察双藤痹痛凝胶膏剂对胶原诱导性关节炎（CIA）模型大鼠血浆炎性细胞因子和滑膜组织Toll样受体4（TLR4）、髓样分化因子88（MyD88）、核因子-κB（NF-κB）表达的影响，探讨其相关作用机制。方法 大鼠尾部皮下注射胶原乳剂制作CIA模型。大鼠随机分为正常组、模型组和双藤痹痛凝胶高、低剂量组，ELISA检测血浆肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）-1β及IL-6的含量，RT-PCR检测滑膜组织TLR4、MyD88、NF-κB的mRNA表达，Western blot检测滑膜组织TLR4、MyD88、NF-κB的蛋白表达。结果 与模型组比较，双藤痹痛凝胶高、低剂量组大鼠血浆TNF-α、IL-1β及IL-6含量显著降低，滑膜组织TLR4、MyD88、NF-κB mRNA和蛋白表达明显降低。结论 双藤痹痛凝胶膏剂可能是通过抑制炎症反应、调节滑膜组织TLR4/NF-κB信号通路相关靶点表达发挥抗炎作用。

关键词：双藤痹痛凝胶膏剂；胶原诱导性关节炎；Toll样受体4；髓样分化因子88；核因子-κB；大鼠

DOI：10.3969/j.issn.1005-5304.2018.01.010

中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2018）01-0043-05

Effect of Shuangteng Bitong Cataplasm on Inflammatory Cytokines in Plasma and Relevant Factors of Rats with Collagen-induced Arthritis

YAN Guo-hong1， ZHANG Yu-qin2， LIN Xiong1， HUANG Yan1， WENG Shu-qin1， XU Wei2

1. Peoples Hospital Affiliated to Fujian University of Traditional Chinese Medicine， Fuzhou 300122， China；

2. School of Pharmacy， Fujian University of Traditional Chinese Medicine， Fuzhou 350108， China

Abstract： Objective To study the effect of Shuangteng Bitong Cataplasm on TLR4， MyD88 and NF-κB and inflammatory cytokines in rats with collagen-induced arthritis； To discuss relevant mechanism of action. Methods A CIA model was made by subcutaneous injection of collagen emulsion from rat tail. Rats were randomly divided into normal group， model group， Shuangteng Bitong Cataplasm high-dose and low-dose groups. The contents of TNF-α， IL-1β and IL-6 in plasma were evaluated by ELISA， and the expression levels of TLR4， MyD88 and NF-κB in synovial tissues were evaluated by RT-PCR and Western blot. Results Compared with the model group， the contents of TNF-α， IL-1β and IL-6 in serum decreased remarkably in the Shuangteng Bitong Cataplasm low-dose and high-dose group， and the expression levels of TLR4， MyD88 and NF-κB in synovial tissues decreased remarkably. Conclusion Shuangteng Bitong Cataplasm can inhibit inflammatory reaction and regulate the expressions of relevant factors in synovial tissues to realize the anti-flammatory effects.

Keywords： Shuangteng Bitong Cataplasm； collagen-induced arthritis； TLR4； MyD88； NF-κB； rats

類风湿关节炎（rheumatoid arthritis，RA）是一种以各关节病变为主，同时伴多系统受累的慢性炎症性的全身性自身免疫性疾病。其病理改变主要发生在关

基金项目：国家自然科学基金（81370096）；福建省科技厅引导性项目（2015Y0060）

通讯作者：徐伟，E-mail：xwfjlab@163.com

节，主要表现为关节腔积液、滑膜炎、炎症细胞浸润、微血管新生、血管翳的形成及软骨和骨组织的破坏。目前RA的病因尚未完全阐明，一般认为是多因素诱发机体自身免疫反应而致病。Toll样受体（TLRs）在免疫系统中发挥着重要的作用，能识别外源性病原相关分子模式及内源性分子产生的组织损伤、炎症反应，通过……