邹俊驹+吴佳敏+贺又舜+伍参荣+赵国荣

摘要：目的 觀察甘露消毒丹及其拆方对柯萨奇病毒A16型（CoxA16）小鼠模型P-选凝素糖蛋白配体1（PSGL-1）和促炎因子的影响，探讨其抗病毒的作用机制。方法 7日龄ICR小鼠150只，随机分为正常组、模型组、全方组、芳残方组、清残方组和利残方组，每组25只。除正常组外，其余各组小鼠腹腔注射20 μL 107TCID50的CoxA16标准株原液进行造模。除正常组和模型组外，其余各组给予相应药物干预。于给药后第10日取材，检测PSGL-1、肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）-1β、IL-4的表达，并进行病理组织观察。结果 除正常组外，其余各组小鼠肌肉组织内存在大量CoxA16，表明造模成功；HE染色结果显示，甘露消毒丹及其拆方可减轻CoxA16对肌肉的损伤。与正常组比较，模型组PSGL-1蛋白表达明显升高（P<0.01）；与模型组比较，全方组、芳残方组、清残方组和利残方组不同程度抑制PSGL-1蛋白的表达，减少致炎因子TNF-α、IL-1β、IL-4的产生（P<0.01）。结论 甘露消毒丹及其拆方具有抗炎作用，其中以全方组效果最明显。

关键词：甘露消毒丹；拆方；P-选凝素糖蛋白配体1；促炎因子；小鼠

DOI：10.3969/j.issn.1005-5304.2017.12.011

中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2017）12-0042-05

Effects of Ganlu Xiaodu Pills and Its Residues on PSGL-1 and Proinflammatory Cytokines in CoxA16 Mouse Model ZOU Jun-ju， WU Jia-min， HE You-shun， WU Can-rong， ZHAO Guo-rong （College of TCM， Hunan University of Chinese Medicine， Changsha 410208， China）

Abstract： Objective To investigate the effects of Ganlu Xiaodu Pills and its residues on PSGL-1 and proinflammatory cytokines in Coxsackie virus A16 （CoxA16） mouse model； To discuss its antiviral mechanism of action. Methods Totally 150 ICR mice at age of 7 days were randomly divided into normal group， model group， all-side group， aromatic residual group， clearing residual group and removing residual group， with 25 mice in each group. Except for normal group， other groups were injected intraperitoneally with 20 μL of 107TCID50 CoxA16 standard stock solution to establish models. Except for normal group and model group， other groups were given relevant medicine for intervention. The expressions of PSGL-1， TNF-α， IL-1β and IL-4 and histopathological observation were detected after 10 days of medication. Results Except for the normal group， the existence of a large number of CoxA16 in other groups of mouse muscle tissues proved successful modeling. HE staining showed that Ganlu Xiaodu Pills and residual could reduce damage to the muscle by CoxA16 virus. Compared with the normal group， the expression of PSGL-1 protein in the model group increased （P<0.01）； compared with the model group， all-side group， aromatic residue group， clearing residual group， removing residual group inhibited the expression of PSGL-1 protein， reduced the inflammatory factors of TNF-α， IL-1β， and IL-4 （P<0.01）. Conclusion Ganlu Xiaodu Pills and its residues have anti-inflammatory effects， and the all-side group shows the best efficacy.

Key words： Ganlu Xiaodu Pills； residual； PSGL-1； proinflammatory cytokines； miceendprint

手足口病是一类由肠道病毒引起的急性传染病，

基金项目：国家自然科学基金（81173188）

通讯作者：贺又舜，E-mail：youshunh@sina.com

其中柯萨奇病毒A组16型（CoxA16）和肠道病毒71型（EV71）2种最为常见[1]。近年来，手足口病在我国出现多次大范围爆发，对我国儿童的健康造成严重威胁。因此对于手足口病的临床特征及最常见病原体的生物学特性、致病机理及疫苗开发等研究日益关注。前期研究发现，甘露消毒丹能抑制柯萨奇病毒在细胞内的复制，并且能在细胞外影响病毒的吸附和穿入[2-3]，通过调节淋巴细胞亚群，诱导IFR3的表达，刺激免疫细胞分泌干扰素（IFN）-γ，达到抗肠道病毒目的[4]。……