皇甫咪咪，曲建强，李 燕，徐 阁，石 军，杜军兰，侯纯扬

1.天津大学理学院，天津 300072

水杨酸钠-次氯酸钠法海水氨氮快速检测方法研究

皇甫咪咪1,2，曲建强1，李 燕2，徐 阁3，石 军3，杜军兰2，侯纯扬2

1.天津大学理学院，天津 300072

2.国家海洋技术中心，天津 300111

3.海口海洋环境监测中心站，海南 海口 570311

对水杨酸钠-次氯酸钠法测量海水氨氮化学分析条件进行了研究。通过正交实验对水杨酸钠-次氯酸钠法试剂配比进行优化，并确定了最佳试剂配比。同时，用水杨酸钠-次氯酸钠法和靛酚蓝法测量24个海水水样，测量结果无显著差异。在反应温度为35 ℃，反应时间为10 min时，用改进后的方法分别测量60、120 μg/L的氨氮标准溶液，相对标准偏差分别为4.0%、2.8%，回收率分别为99.8%、100.8%。该方法试剂无毒、环保，操作简单、快速，可满足营养盐自动分析仪现场快速测量的要求，提高仪器的环境友好性。

水杨酸钠-次氯酸钠；氨氮；海水；环境友好

Abstract:The detection of ammonia nitrogen in seawater through the method of Sodium Salicylate and Sodium Hypochlorite was studied in this paper. The reagent ratios were optimized by the orthogonal experiment and the best reagent ratio was determined. Ammonia nitrogen in 24 seawater samples was determined with Sodium Salicylate and Sodium Hypochlorite method and indophenol blue method. The results showed that the two methods gave similar values and did not have significant difference. Under the reaction of 10 minutes at the temperature of 35 ℃, the standard ammonia nitrogen solution of 60 and 120 μg/L were detected by the optimized method, while the standard deviation were 4.0% and 2.8%, and recovery were 99.8% and 100.8%, respectively, which can reach the demands of auto-analyzing technology on the spot. The new method is convenient and environment-friendly.

Keywords:sodium salicylate and sodium hypochlorite;ammonia nitrogen;seawater;environmentally friendly

目前，海水中氨氮测量采用的方法主要有荧光法[4]、次溴酸钠氧化法[5]、靛酚蓝法[6-8]、水杨酸钠法等。荧光法灵敏度高，主要用于大洋中nmol/L浓度水平氨氮的测量[9]。次溴酸钠氧化法和靛酚蓝法技术成熟，重现性好，准确度高，是《海洋监测规范》(GB 17378.4—2007)[10]规定的适用于大洋和近岸海水中氨氮测量的标准方法，但是次溴酸钠氧化法容易受到氨基酸的干扰，所用试剂次溴酸钠溶液稳定性差，需要现用现配，使其在现场仪器测量中的应用受到局限。水杨酸钠分光光度法是测量水中氨氮常用分析方法，国家环境保护……