郑斌，吴齐全，周克文，王钢，任雨，祁洪刚，朱伟智，翁国斌

（宁波市鄞州第二医院 泌尿外科，浙江 宁波 315100）

转录因子SOX7对前列腺癌PC-3细胞生物学活性的影响及其机制

郑斌，吴齐全，周克文，王钢，任雨，祁洪刚，朱伟智，翁国斌

（宁波市鄞州第二医院 泌尿外科，浙江 宁波 315100）

目的：探讨转录因子SOX7对前列腺癌PC-3细胞增殖、细胞周期、迁移和侵袭能力的影响及其机制。方法：采用脂质体法转染高表达SOX7重组质粒（pCDNA3.1-SOX7），以转染空质粒（Vector）作为对照，检测SOX7对PC-3细胞株增殖、细胞周期、迁移及侵袭的影响，并用Western blot法检测相关蛋白表达水平。结果：pCDNA3.1-SOX7显著抑制PC-3细胞增殖能力，并且诱导细胞周期发生G1期阻滞，同时，使Cylcin D1、E表达量明显下调。此外，pCDNA3.1-SOX7转染后，PC-3细胞迁移距离显著减少，穿透基质胶的细胞数量也显著降低，并且伴随着间质表型标记物MMP-2、MMP-9和N-cadherin蛋白表达减少，上皮标记物E-cadherin蛋白表达增加。结论：SOX7抑制前列腺癌PC-3细胞的增殖、周期、迁移及侵袭能力，其机制可能与调控这些细胞行为的相关蛋白表达有关。

SOX7；前列腺癌；增殖；迁移；侵袭

Abstract: Objective:To investigate the effects and mechanisms of transcription factor SOX7 on PC-3 cells proliferation, cell cycle, cell migration and invasion.Methods:PC-3 cells were transfected with highexpressed SOX7 recombinant plasmid (pCDNA3.1-SOX7) and further detected the changes of cell proliferation,cell cycle, migration, invasion, and related protein expressions.Results:After transfection with pCDNA3.1-SOX7, significant inhibition effects was shown in the PC-3 cell proliferation and G1/S cell cycle arrest, which were accompanied with decreased expressions of cyclin D1 and E. Furthermore, transfection of pCDNA3.1-SOX7 markedly decreased the cell migration distances and invasion capacity, with a reduction of the mesenchymal markers (MMP-2, MMP-9 and N-cadherin), however, the epithelial marker E-cadherin was increased.Conclusion:SOX7 inhibits PC-3 cell proliferation, cell cycle, migration and invasion through regulation of its related protein expressions.

Key words:SOX7; prostate cancer; proliferation; migration; invasion

前列腺癌为西方男性人群最常见的恶性肿瘤之一，近年来在我国的发病率与病死率呈逐年上升趋势[1-2]。临床上一般采取手术切除和放射疗法为主，然而肿瘤复发与转移问题已成为目前治疗的难点。上皮间质转化（epithelial-mesenchymal transition，EMT）过程是肿瘤侵袭和转移的关键环节。在此过程中，上皮细胞表型转化为成纤维细胞的间质表型，上皮细胞间紧密连接相关蛋白表达下调，细胞外基质增多，抗凋亡能力增强，从而促进细胞迁移与侵袭[3]。……