薛梅 周卫平

摘要[目的]探讨龙牙百合鳞茎总黄酮的最佳提取工艺，并研究其抗氧化活性。[方法]以龙牙百合鳞茎为研究对象，采用优化超声波法提取总黄酮。在单因素试验的基础上，采用正交试验法研究了乙醇浓度、料液比、提取时间、提取温度对总黄酮提取量的影响，并确定其最佳工艺条件，并对其体外抗氧化活性进行了研究。[结果]龙牙百合鳞茎总黄酮的最佳工艺条件如下：提取温度为30 ℃，乙醇浓度为80%，料液比（g ∶mL）为1∶10，提取时间为20 min。在此条件下，百合鳞茎总黄酮提取量为（32.236±0.513）mg/g。抗氧化试验结果表明，龙牙百合鳞茎总黄酮提取液对DPPH· 的清除效果优于相同浓度的维生素C溶液，且存在顯著差异（P<0.05）。[结论]该研究可为龙牙百合的综合开发与利用提供理论依据。

关键词 龙牙百合鳞茎；总黄酮；超声波法；抗氧化活性

中图分类号 S644.1 文献标识码 A 文章编号 0517-6611（2017）03-0010-03

Abstract[Objective] To explore the optimum extraction technology of total flavonoids from Longya lilium bulb and study their antioxidant activity.[Method] Taking L.lilium bulb as research object，total flavonoids were extracted from L.lilium bulb by using optimized ultrasonic method.Based on single factor experiment，the effects of ethanol concentration，materialliquid ratio，extraction time and extraction temperature on the extraction quantity of total flavonoids from L.lilium bulb were studied and the optimum extraction technology was confirmed by using orthogonal experiment.And their antioxidant activities of total flavonoids in vitro were studied.[Result] The optimum extraction conditions of total flavonoids from L.lilium bulb were as follows：80% ethanol，solidliquid ratio of 1∶10 （g ∶mL），extraction temperature of 30 ℃，extraction time of 20 min.Under the optimum conditions，the extraction quantity of total flavonoids was （32.236±0.513） mg/g.The results of in vitro antioxidant test showed that the scavenging effects of total flavonoids extract from L.lilium bulb to DPPH· was better than that of vitamin C solution at the same concentration，and there was significant difference（P<0.05）.[Conclusion] The research can provide theoretical basis for the comprehensive exploration and utilization of L.lilium.

Key words Longya lilium bulb；Total flavonoids；Ultrasonic method；Antioxidant activity

龍牙百合（Lilium brownii var.viridulum）为多年生草本植物，是我国重要的经济型食用百合品种之一，主产于四川、湖南、重庆等地[1]。龙牙百合食用部分为鳞茎，且具有多种药效，营养价值很高，是卫生部首批公布的药食同源食品[2]。

黄酮类化合物又名生物类黄酮（Bioflavonoids），包括黄酮、异黄酮、黄烷酮、双黄酮及其甙类，具有广泛的生理活性，广泛存在于果蔬和中草药中。黄酮具有清除自由基、抗脂质过氧化、提升体内抗氧化水平的作用，此外还具有作为防治心脑血管疾病的天然药物和膳食补充剂开发的潜力[3]。

目前，关于植物黄酮提取工艺的研究已经涉及多种植物[4-7]，但关于龙牙百合鳞茎的研究还较少。笔者以龙牙……