陈奔+覃梅春+黄金兰+吴登攀+郭尔楚+刘志萍+许哲郝++郭茜茜+钟振国

[摘要]初步建立老年性痴呆血瘀证病证结合树鼩动物模型并以三七总皂苷进行药物干预，以该药物反证该病证结合模型的稳定性。以5种中医表征分析方法及凝血4项指标、血浆NO含量、血浆SOD含量评价各组树鼩模型的血瘀证水平；以免疫印迹试验（Western blot）检测各组树鼩大脑Aβ142蛋白、淀粉样蛋白前体蛋白（APP）、磷酸化Tau蛋白（PTau）含量；HE染色观察各组树鼩大脑海马神经细胞形态，免疫组化法检测各组树鼩大脑海马胆碱乙酰基转移酶（ChAT）与突触素（SYP）含量。通过实验得出病证结合组树鼩血瘀证中医表征较AD组显著，给药组树鼩血瘀证表征较病证结合组减轻；与空白组相比，AD組树鼩大脑中Aβ142，APP及PTau含量降低，ChAT及SYP的平均阳性截面积/A降低，病证结合造模使这一降低加重，而三七总皂苷给药缓解了这一变化。说明AD血瘀证病证结合树鼩模型具有稳定性。

[关键词]老年性痴呆病； 血瘀证； 病证结合动物模型； 树鼩； 三七总皂苷

[Abstract] To establish the integration of Alzheimer′s disease（AD） and blood stasis syndrome tree shrew model. Panax notoginseng saponins （PNS） was used to intervene the model to testify the stability of the model. The level of blood stasis of each group in the tree shrew model was evaluated by analyzing five traditional Chinese medicine（TCM） characterizations， four blood coagulation indexes， plasma nitric oxide （NO） level， plasma superoxide dismutase （SOD） level in each group. Hematoxylin and eosin（HE） staining was used to observe the morphological changes of brain hippocampal neuron cell of each group. Immunohistochemical staining was used to assay the ChAT and SYP levels in brain hippocampus of each group.The blood stasis characterization of the integration of disease and syndrome group was more obvious than the AD group， and that of the drug administration group was lower than that of the integration of disease and syndrome group. Aβ142， APP， PTau， ChAT and SYP level of AD group were lower than those in the blank group， which were further reduced in the model of integration of disease and syndrome. However， the administration of PNS relieved the reduction， indicating that the AD and blood stasis integration syndrome tree shrew model is stable.

[Key words]Alzheimer′s disease； blood stasis syndrome； integration of disease and syndrome animal model； tree shrew； Panax notoginseng saponins

阿尔茨海默病（Alzheimer′s disease）是一种慢性进行性智力损害疾病，被认为是在老年群体中最普遍的一种痴呆类型[1]。主要表现为年老者语言、记忆、认知、情感、性格和行为的功能性缺失。目前估计已有3 600万人患上AD，这个数字预计到2050年会变为11 800万[2]。AD已经逐渐成为社会和医药学科发展的主要问题。先前的研究显示了AD的病理特征包括细胞外沉积老年斑，细胞内神经原纤维缠结的形成，产生在大脑皮层、海马和大脑其他区域的胆碱能神经元损害及突触改变[3]。……