努尔比艳木?阿布都热合曼+塔依尔?吐尔松+买吾拉尼江?依孜布拉+马丽娜+毛吾丽旦木?迪力夏提+阿米尔?艾力+努尔买买提?艾买提

摘要：目的 通過代谢组学方法，研究咸味黏液质成熟与清除疗法对咸味黏液质型银屑病患者体内物质代谢的影响。方法 采用核磁共振（NMR）技术测定维药咸味黏液质成熟与清除疗法治疗前后咸味黏液质型银屑病患者及健康志愿者血清代谢物，使用正交偏最小二乘法判别分析研究血清内源性化合物在治疗前组、治疗后组与正常对照组间的差异。结果 与正常对照组比较，治疗前组血清中极低密度脂蛋白、异亮氨酸、亮氨酸、缬氨酸、苏氨酸、丙氨酸、蛋氨酸、乳酸、乙酸、糖蛋白、乙酰半胱氨酸、谷氨酰胺、丙酮、肉碱、肌酸、丙二酸、β-葡萄糖、α-葡萄糖、肌醇、胆碱、甘氨酸、低密度脂蛋白明显升高，酪氨酸、组氨酸、甲酸明显降低（P<0.05）。与治疗前组比较，治疗后组血清中β-葡萄糖、α-葡萄糖明显降低，差异有统计学意义（P<0.05）。结论 咸味黏液质型银屑病患者血清中氨基酸代谢、脂类代谢、糖代谢等出现不同程度异常。维药咸味黏液质成熟与清除疗法可能通过调节患者体内紊乱的糖代谢来实现治疗作用。

关键词：成熟与清除疗法；咸味黏液质；银屑病；代谢组学

DOI：10.3969/j.issn.1005-5304.2017.04.007

中图分类号：R291.5；R275.986.3 文献标识码：A 文章编号：1005-5304（2017）04-0024-05

Effects of Uyghur Medicine Munziq and Mushil's Therapy on Serum Metabolomics of Abnormal Phlegmatic Type Psoriasis Nuerbiyanmu Abudureheman1，2， Tayirjan Tursun1， Maiwulanijiang Yizibulla1， MA Li-na3， Maowulidanmu Dilixiati1， Amier Aili2， Nurmuhamat Amat1 （1. College of Traditional Uyghur Medicine of Xinjiang Medical University， Urumqi 830011， China； 2. Hospital of Traditional Uyghur Medicine of Aksu Region， Aksu 843000， China； 3. Central Laboratory of Xinjiang Medical University， Urumqi 830011， China）

Abstract： Objective To investigate the effects of Uyghur Medicine Munziq and Mushils therapy on the metabolic changes in serum of patients with abnormal phlegmatic type psoriasis by NMR based metabonomic method. Methods NMR technology were used to detect serum of patients with abnormal phlegmatic type psoriasis before and after treated by Munzig and Mushils therapy. OPLS-DA was used to study the differences of serum endogenous compounds between pre-treatment， post-treatment and normal control groups. Results Compared with normal control group， levels of some metabolites including VLDL， isoleucine， leucine， valine， lactate， threonine， alanine， methionine， acetic acid， glycoprotein， acetylcysteine， glutamine， acetone， carnitine， creatine， malonic acid， β-glucose， α-glucose， inositol， choline， glycine and LDL in serum increased significantly， while levels of tyrosine， histidine， and formic acid decreased significantly （P<0.05）. Compared with before treatment， levels of β-glucose and α-glucose decreased significantly after treatment， with statistical significance （P<0.05）. Conclusion The levels of amino acid metabolism， lipid metabolism and glucose metabolism in patients with abnormal phlegmatic type psoriasis are abnormal to different degrees. The treatment of Uyghur Medicine Munziq and Mushils therapy may be achieved through regulating the disorder of glucose metabolism in the patient.endprint

Key words： Munziq and Mushil's therapy； abnormal phlegm； psoriasis； metabonomics

銀屑病（psoriasis）是一种常见的免疫介导的慢性炎症性复发性皮肤病，世界范围内自然人群发病率为2%～3%[1-2]。其发病机制迄今未明确，尚无有效的治疗方法，研究有效的防治方法已成为众多医学工作者面临的一项迫切任务。体液论是维吾尔医学的核心，胆液质、黏液质、血液质、黑胆质4种体液的异常变化都可导致银屑病，其中黏液质的异常变化最常见。代谢组学可以反映生命个体对外源性物质的刺激、环境变化或遗传修饰所作出的所有代谢应答的全貌和动态变化过程。……