赵国荣*，张衎，何宜荣*，艾碧琛，肖碧跃，贺又舜，左亚杰，熊焰，陈兰玲，尹周安

（1.湖南中医药大学中医学院，湖南长沙410208；2.广西中医药大学第一附属医院，广西南宁530023；3.湖南中医药大学第一附属医院，湖南长沙410007）

清热解毒化浊片对ETM兔肝组织CD14及TNF-α、IL-1表达的影响

赵国荣1*，张衎2，何宜荣1*，艾碧琛1，肖碧跃1，贺又舜1，左亚杰3，熊焰1，陈兰玲3，尹周安1

（1.湖南中医药大学中医学院，湖南长沙410208；2.广西中医药大学第一附属医院，广西南宁530023；3.湖南中医药大学第一附属医院，湖南长沙410007）

目的探讨清热解毒化浊片对内毒素血症（Endotoxemia，ETM）模型兔肝功能、肝组织CD14和血清肿瘤坏死因子-α（TNF-α）、白细胞介素-1（IL-1）的影响。方法将21只新西兰大耳白兔随机分为正常组、模型组、清热解毒化浊片组（清化组），每组7只。将模型组及清化组兔沿耳缘静脉注射大肠杆菌内毒素（LPS，10μg/kg），于造模后2 h干预，灌胃后2 h心脏采血检测家兔的肝功能、TNF-α和IL-α的变化，采肝脏组织检测CD14及观察肝脏病理形态。结果（1）与模型组比较，清化组ALT、AST上升（P＜0.05），肝组织CD14表达及血清TNF-α、IL-1下降（P＜0.05）；（2）模型组肝细胞排列结构紊乱，有炎性细胞浸润，且出现空泡样改变，清化组及正常组改变不明显。结论清热解毒化浊片能下调ETM模型兔CDl4、TNF-α和IL-1的表达，具有多靶点、多效应的干预作用，推测其有阻断早期LPS诱导信号通路的作用。

内毒素血症；清热解毒化浊片；CD14；肿瘤坏死因子-α；白细胞介素-1；茵陈；黄芩；滑石

Effect of Qingre Jiedu Huazhuo Tablets on Expression of CD14 in Liver Tissue，TNF-αand IL-1 of ETM Rabbits

〔Abstract〕Objective To investigate the effect of Qingre Jiedu Huazhuo（QJH）tablets on the expression of CD14，TNF-αand IL-1 in liver tissue of ETM rabbits.M ethods The 21 New Zealand big-eared rabbits were random ly divided into normal group，model group，QJH tablets group，7 in each group.The ear edge of model group and QJH group was given intravenous injection of 10μg/kg endotoxin.After 2 hours of modeling，the rabbites were given intervention.Then，the liver function，TNF-αand IL-1 were tested.The CD14 in liver tissue was determined and the pathological morphology was observed.Results（1）Compared with the model group，the ALT and AST of QJH tablets group were increased（P＜0.05）.The CD14 expression in liver tissue and serum TNF-αand IL-1 were decreased（P＜0.05）.（2）The liver cell array structure of model group was disorder with inflammatory cells infiltration and a cavity change，but the changes in QJH tablets group and blank group were not obvious.Conclusion Qingre Jiedu Huazhuo tablets with multi-point target and munti-effects can decrease CDl4，TNF-αand IL-1 in ETN rabbits，suggesting the tablets have an effect on the signaling pathway induced by LPS in the early days.

〔Keywords〕endotoxemia；Qingre Jiedu Huazhuo tablets；CD14；TNF-a；IL-1；oriental wormwood；radix scutellariae；talc

研究团队前期对清热解毒化浊片（简称化浊片）进行了大量临床及实验研究，证实其不仅可用于治疗病毒性肝炎肝损伤，还可清除内毒素［1-3］。……