李岩岩，何泽云*，吴　凡，周艳利

（1.郑州市第三人民医院，河南郑州450000；2.湖南中医药大学第一附属医院，湖南长沙410007；3.广州市中医医院，广东广州510130）

六味地黄汤对5/6肾切除大鼠NF-κB、MCP-1、Col-Ⅲ表达的影响

李岩岩1，何泽云2*，吴凡1，周艳利3

（1.郑州市第三人民医院，河南郑州450000；2.湖南中医药大学第一附属医院，湖南长沙410007；3.广州市中医医院，广东广州510130）

目的观察六味地黄汤对5/6肾切除大鼠残肾转录因子-核因子κB（NF-κB）、单核细胞趋化因子（MCP-1）及Ⅲ型胶原（Col-Ⅲ）表达的影响，探讨六味地黄汤抑制肾间质纤维化进展的作用机制。方法按照随机数字表法将60只SD雄性大鼠分为5组：空白组、假手术组、模型组、依那普利组、六味地黄汤组，每组12只，后三组行5/6肾切除术诱导大鼠肾衰模型，假手术组同期手术，但不损及肾脏。造模术后3 d进行灌胃干预。灌胃8周后处死各组大鼠，观察各组大鼠残肾组织形态学变化并用免疫组化法检测大鼠残肾NF-κB、MCP-1、Col-Ⅲ的表达。结果（1）观察5/6肾切除大鼠残肾组织细胞形态学，与模型组相比六味地黄汤、依那普利组可减轻大鼠肾间质损害及纤维化程度；（2）免疫组化半定量分析显示六味地黄汤组肾皮质的NF-κB、MCP-1及Col-Ⅲ表达均明显低于模型组（P＜0.05），与依那普利组差异无统计学意义（P＞0.05）。结论六味地黄汤可能通过下调NF-κB、MCP-1及Col-Ⅲ的表达，减少细胞外基质的积聚，减轻炎症反应和纤维化程度，抑制5/6肾切除大鼠肾间质纤维化进展，可作为慢性肾衰竭的辅助用药。

六味地黄汤；5/6肾切除；转录因子-核因子κB；单核细胞趋化因子；Ⅲ型胶原

〔Abstract〕Objective To observe the effects of Liuwei Dihuang decoction on the expression of nuclear factor kappa B（NF-κB），monocyte chemoattractant protein-1（MCP-1）and collagen type III（Col-Ⅲ）in remnant kidney of 5/6 nephrectmized rats，and to investigate the mechanism of action for Liuwei Dihuang decoction on renal tubulointerstitial fibrosis.M ethods The sixty male Sprapue-Dewley rats were randomly divided into five groups：blank group，sham-operation group，model group，enalapril group and Liuwei Dihuang decoction group，12 rats in each group.The model group，enalapril group and Liuwei Dihuang decoction group adopted 5/6 nephrectomized induced into renal failure model.After 3 days of modeling，the rats were treated by gavage.After 8 weeks，rats were sacrificed，remnant kidney tissue morphological changes were observed and the expression of NF-kB，MCP-1，Col-Ⅲlevels were tested by immunohistochemistry.Results（1）The cell morphology in remnant kidney of 5/6 nephrectmized rats was observed.Compared with model group，renal tubulointerstitial damage and fibrosis was reduced in Liuwei Dihuang decoction and enalapril group；（2）Compared with model group，NF-κB，MCP-1 and Col-III expression level were reduced markedly in Liuwei Dihuang decoction group（P＜0.05），there is no obvious diffrencebetween Liuwei Dihaung group and enalapril group（P＞0.05）.Conclusion Liuwei Dihuang decoction may inhibit the expression of NF-κB，reduce the expression of MCP-1，decrease the accumulation of extracellular matrix，reduce the inflammation and fibrosis and delay the progress of renal interstitial fibrosis，which can be the adjuvant drug of chronic renal failure.

〔Keywords〕Liuwei Dihuang decoction；5/6 nephrectomy；nuclear factor kappa B；monocyte chemoattractant protein-1；collagen type III

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