肖小芹，贺艳萍，邓桂明*，陈　镇，李晓屏，欧阳林旗，肖望重，何海，舒圆月，向彪

（1.湖南中医药大学，湖南长沙410208；2.湖南中医药大学第一附属医院，湖南长沙410007）

·方药研究·

咳喘穴位敷贴对哮喘大鼠Th1/Th2免疫平衡的影响

肖小芹1，贺艳萍1，邓桂明2*，陈镇2，李晓屏2，欧阳林旗2，肖望重2，何海2，舒圆月1，向彪2

（1.湖南中医药大学，湖南长沙410208；2.湖南中医药大学第一附属医院，湖南长沙410007）

目的探讨咳喘穴位敷贴对哮喘大鼠辅助性T淋巴细胞1（Th1）/辅助性T淋巴细胞2（Th2）免疫平衡的影响。方法60只雄性SD大鼠随机分为6组：正常对照组、哮喘模型组、生芥子组、生炒各半组、炒芥子组、地塞米松组，每组10只。采用卵清蛋白致敏并激发的方法制备大鼠慢性哮喘模型，白芥子不同配比咳喘穴位敷贴散贴敷穴位干预治疗。采用酶联免疫吸附剂测定法（ELISA）测定各组大鼠血清中干扰素（IFN）-γ、白介素（IL）-4、白介素（IL）-13的含量。结果与正常对照组比较，哮喘模型组大鼠血清IFN-γ含量明显降低，而IL-4、IL-13的含量明显升高（P＜0.01）。与哮喘模型组比较，生芥子组、生炒各半组、炒芥子组和地塞米松组的大鼠血清IFN-γ含量明显升高，而IL-4、IL-13的含量明显降低（P＜0.01），各治疗组之间差异无统计学意义（P＞0.05）。结论咳喘穴位敷贴能够调节哮喘大鼠Th1/Th2免疫平衡，改善炎症反应，从而改善哮喘病情。

哮喘；穴位敷贴；白芥子；Th1；Th2

〔Abstract〕Objective To discuss the effects of kechuan acupoint application on Th1/Th2 cells immune balance of asthma rats.M ethods 60 male SD rats were random ly divided into control normal group，asthma model group，raw mustard group，half raw and half roasted mustard group，roasted mustard group，dexamethasone group，and with 10 rats in each group.The chronic asthma rat models were established by using ovalbumin to sensitize and stimulate，different proportion of white mustard acupoint application powder was as the intervention treatment.The levels of IFN-γ，IL-4 and IL-13 in serum of rats were detected by using ELISA.Results Compard with control group，the level of IFN-γin serum of rats in model group was decreased significantiy，while the level of IL-4，IL-13 in serum of rats in model group was increased significantiy（P＜0.01）.Compard with asthma model group，the IFN-γlevels in raw mustard group，half raw and half roasted mustard group，roasted mustard group，dexamethasone group were increased obviously，while the IL-4，IL-13 levels of rats in these groups were decreased（P＜0.01）.The results had no significant difference between the treatment group（P＞0.05）. Conclusion Kechuan acupoint application may adjust Th1/Th2 cells immune balance of asthma rats，to imporve the inflammatory response，and improve asthma condition.

〔Keywords〕asthma；acupoint application；white mustard；Th1；Th2

支气管哮喘是由多种细胞（如嗜酸性粒细胞、肥大细胞、T淋巴细胞等）和细胞组分参与的气道慢性炎症性疾病［1］。哮喘是世界上最常见的慢性疾病之一，目前全球哮喘患者已经达到3亿，各国哮喘患病率从1%～30%不等，我国约为0.5%～5%，尤其是儿童和青少年患病率高［2］，是严重危害人类健康和生活质量的常见病和多发病。……