晁　旭王越月杜　静赵家荣安庆玲李磊磊

（1.陕西中医药大学基础医学院，陕西　咸阳712046；2.陕西中医药大学第二附属医院，陕西咸阳71200）

·研究报告·

加味苓桂术甘方对酒精性脂肪肝大鼠肝脏组织ADRP、PPAR-γ和Sir 2蛋白表达的影响*

晁旭1，2王越月2杜静2赵家荣1安庆玲1李磊磊1

（1.陕西中医药大学基础医学院，陕西咸阳712046；2.陕西中医药大学第二附属医院，陕西咸阳71200）

目的研究加味苓桂术甘方对酒精性脂肪肝大鼠肝脏组织脂肪分化相关蛋白（ADRP）、过氧化物酶体增殖活化物受体γ（PPAR-γ）和沉默信息调控子2（Sir2）蛋白表达的影响。方法SD雄性大鼠60只，随机分为6组，每组10只。50只给予含乙醇灌胃，同时饲喂高脂饲料，制备酒精性脂肪肝模型。实验组动物给予不同剂量的加味苓桂术甘灌胃6周。然后采用生化分析仪检测血样中总胆固醇（CHOL）、甘油三酯（TG）、脂肪酸（FFA）的含量；利用实时荧光定量聚合酶链式反应（RT-PCR）和免疫印迹（Western blot）技术，检测酒精性脂肪肝大鼠模型肝脏组织中ADRP、PPAR-γ和Sir2的mRNA和蛋白表达。结果与模型组相比，给药6周后加味苓桂术甘方中剂量和大剂量组大鼠血清中TG、CHOL和FFA水平均有明显下降（P＜0.05）；加味苓桂术甘方可显著下调肝脏组织中ADRP、PPAR-γ的表达，而上调Sir2的表达。结论加味苓桂术甘方可通过改变肝脏组织ADRP、PPAR-γ和Sir2的表达，从而抑制酒精性脂肪肝的发生。

酒精性脂肪加味苓桂术甘方ADRP PPAR-γSir2

【Abstract】Objective：To study effects of Jiawei Linggui Zhugan Decoction on expression of ADRP，PPAR-γ and Sir 2 protein in liver tissues in ratmodelwith alcoholic fatty liver.M ethods：60male SD ratswere random ly divided into 6 groups with 10 rats in each group.50 rats were fed with high fat diet and ethanol fed to establish the experimentalmodel of alcoholic fatty liver.The animals of the experimental group were treated with different doses of Jiawei Linggui Zhugan Decoction and ethanol gavage.The total cholesterol（CHOL），triglyceride（TG）and fatty acid（FFA）in blood of animalsweremeasured with biochemical analyzer.The expressions of ADRP，PPAR-γand Sir2 mRNA and proteins in liver tissues were analyzed through RT-PCR and western blot.Results：Compared with themodel group，the levels of TG，CHOL and FFA in serum of ratmodels，treated with Jiawei Linggui Zhugan Decoction with medium-dosage and the high for 6 weeks，were significantly lower（P＜0.05）.Jiawei Linggui Zhugan Decoction could significantly decrease the expression of ADRP，PPAR-γ，and up-regulated the expression of Sir 2 in liver tissue.Conclusion：Jiawei Linggui Zhugan Decoction can change the expression of ADRP，PPAR-γand Sir 2 in liver tissue，thereby inhibiting the occurrence of fatty liver.

【Key words】Alcoholic fatty liver；Jiawei Linggui Zhugan Decoction；ADRP；PPAR-γ；Sir2

酒精性脂肪肝（AFL）是指由于长期酒精摄入过量而导致的肝脏疾病［1］。随着生活水平的提高，我国酒精性脂肪肝患者急剧增长［2］。AFL在早期是可以逆转的，经戒酒和治疗后，肝损害可逐渐得以改善［3］。……