林云娇王露露吴　洁黄　佳宋长明

林冰冰1，2，3柳维林1，2，3陶　静1，2，3陈立典1，2，3，4△

·研究报告·

电针“曲池”“足三里”穴对缺血再灌注损伤大鼠纹状体DARPP-32磷酸化的影响*

林云娇1，2，3王露露1，2，3吴洁1，2，3黄佳1，2，3宋长明1，2，3

林冰冰1，2，3柳维林1，2，3陶静1，2，3陈立典1，2，3，4△

（1.福建中医药大学康复医学院，福建福州350122；2.福建省康复技术重点实验室，福建福州350122；3福建康复产业研究院，福建福州350122；4.国家中医药管理局中医康复研究中心，福建福州350122）

目的观察电针“曲池”“足三里”穴对脑缺血再灌注损伤局灶性大脑中动脉闭塞模型（MCAO）大鼠神经行为学的变化及多巴胺和环磷酸腺苷调节的蛋白（DARPP-32）磷酸化的影响。方法将25只雄性SD大鼠随机分为假手术组（6只）、手术组（19只）。手术组采用Longa线拴法制备左侧左侧大脑中动脉栓塞（MCAO）大鼠模型，将造模后符合纳入标准的12只大鼠随机分为模型组、电针组，各6只。电针组取“曲池”和“足三里”穴，电针干预14 d，假手术组和模型组在同等条件下抓取及固定，不予任何干预。通过神经功能评分判断大鼠的神经功能缺损情况；小动物磁共振仪（MRI）扫描观察脑梗死体积，Western blot检测纹状体DARPP-32、p-Thr75-DARPP-32的表达情况。结果与模型组相比，电针组大鼠神经功能评分降低（P＜0.05），脑梗死体积减少（P＜0.05），纹状体p-DARPP-32-Thr75/DARPP-32比值增高（P＜0.05）。结论电针“曲池”和“足三里”穴可改善MCAO大鼠神经功能缺损症状，其机制可能与促进缺血纹状体的DARPP-32在Thr75位点的磷酸化有关。

脑缺血电针DARPP-32 p-DARPP-32-Thr75

【Abstract】Objective：To observe the effect of electro-acupuncture at Quchi（LI11）and Zusanli（ST36）on the change of neurological deficit，and the phosphorylation of dopamine and cyclic adenosin 3′，5′-monophosphate regulated phosphoprotein（DARPP-32）in rat after cerebral ischemia reperfusion injury.M ethods：25 male SD ratswere randomly divided into the sham operation group（n=6）and the operation group（n=19）.The operation group were under the operation of the leftmiddle cerebral artery occlusion（MCAO）by themodified Longa′methods，and 12 qualified ratswere randomly divided into themodel group（n=6）and the electro-acupuncture group（n=6）.The electro-acupuncture group received electro-acupuncture at Quchi（LI11）and Zusanli（ST36）for 14 days.The neurological deficit was assessed；the cerebral infarct volume was scanned with animalmagnetic resonance imaging（MRI）machine.The protein expression of DARPP-32 and p-Thr75-DARPP-32 were detected by Western blotting technique.Results：Compared with themodel group，the neurological deficit score in the electro-acupuncture group was improved（P＜0.05）.The size of infarct volume was significantly reduced（P＜0.05）. The radio of p-DARPP-32-Thr75/DARPP-32 in corpus striatum in electro-acupuncture group was higher than that in themodel group（P＜0.05）.Conclusion：The electro-acupuncture at Quchi（LI11）and Zusanli（ST36）can promote the recovery of neurologic deficit symptom，which may be associated with promoting the phosphorylation of DARPP-32 on Thr75.

【Key words】Cerebral ischemia；Electro-acupuncture；DARPP-32；p-DARPP-32-Thr75在我国，脑卒中后幸存患者中70%～80%遗留不同程度的功能障碍［1］，严重响患者的生存质量，同时也带来沉重的经济负担［2］。脑……