鲍月宁　曹传宇宁夏回族自治区固原市人民医院神经内科，宁夏固原　756000

天麻素改善氯化钴诱导大鼠缺氧性脑神经元损伤的效果

鲍月宁曹传宇
宁夏回族自治区固原市人民医院神经内科，宁夏固原756000

目的 研究天麻素对氯化钴诱导大鼠缺氧性脑神经元损伤的修复作用，为缺血缺氧性脑损伤的临床治疗提供实验依据。方法 取SD大鼠大脑皮质神经细胞，原代培养7 d后按照培养皿编号，采用随机数字表法分为空白对照组、模型组、天麻素组及天麻素对照组，并实施相应处理。观察各组细胞形态学、神经元相对活力、乳酸脱氢酶（LDH）释放量、EphA4表达的差异。结果 空白对照组神经元细胞相对活力显著高于其他各组，模型组神经元细胞相对活力显著低于天麻素组、天麻素对照组，天麻素组神经元细胞相对活力显著低于天麻素对照组（P＜0.01）。模型组神经元细胞LDH活性显著高于其他各组，天麻素组、天麻素对照组神经元细胞LDH活性显著高于空白对照组（P＜0.01）。模型组神经元平均累积吸光度（IOD）值显著高于其他各组，空白对照组神经元平均IOD值显著低于其他各组（P＜0.01）。结论 天麻素能够显著降低大鼠缺氧损伤神经元EphA4表达水平，抑制神经元细胞LDH活性，增强细胞相对活力，对大鼠皮质神经元损伤的保护具有积极作用，值得进一步研究。

天麻素；氯化钴；皮质神经元损伤；神经保护；缺血缺氧性脑损伤

［Abstract］Objective To study the repair effect of gastrodin on hypoxic brain neurons damage induced by cobalt chloride in rats，and provide an experimental reference for clinical treatment of ischemic and hypoxic brain damage. Methods Cerebral cortex nerve cells of SD rats were selected for study.The original generation after 7 d，they were numbered according to the culture dish.Primary cells were divided into blank control group，model group，gastrodin group and gastrodin control group by using random number table method，and corresponding treatment was implemented.Cell morphology，neurons relative vigor，lactate dehydrogenase（LDH）release quantity，EphA4 expression difference among four groups were observed.Results The relative activity of neurons in blank control group was significantly higher than that in other groups，the relative activity of neurons in model group was significantly lower than that in gastrodin group and gastrodin control group，the relative activity of neurons in gastrodin group was significantly lower than that in gastrodin control group（P＜0.01）.The neurons LDH activity of model group was significantly higher than that of other groups，the neurons LDH activity of gastrodin group and gastrodin control group were significantly higher than those of blank control group（P＜0.01）.The average neurons IOD of model group was higher than that of other groups，the average neurons IOD of blank control group was lower than that of other groups（P＜0.01）.Conclusion Gastrodin can reduce the expression level of EphA4 in the injured neuron，inhibit the activity of LDH in neuronal cells，and enhance the cell relative viability，has a positive effect on the protection of rat cortical neuronal injury，is worthy of further study.

［Key words］Gastrodin；Cobalt chloride；Cortical neuron injury；Nerve protection；Hypoxic ischemic brain damage

缺血缺氧性脑损伤的病理过程包括能量代谢障碍、细胞内钙超载、兴奋性氨基酸分泌过多及一氧化氮蓄积等，上述病理反应引发的级联细胞毒作用是诱发神经元损伤的主要原因［1］。……