谭宏伟　楚光华　胡春艳　张恩娣西北妇女儿童医院妇科，陕西西安　710061

三七总皂苷对子宫内膜癌Ishikawa和HEC-1A细胞增殖、侵袭和凋亡的影响

谭宏伟楚光华胡春艳张恩娣
西北妇女儿童医院妇科，陕西西安710061

目的 探讨三七总皂苷（PNS）对子宫内膜癌Ishikawa和HEC-1A细胞增殖、侵袭和凋亡的影响及其作用机制。方法 应用不同浓度的PNS分别作用于Ishikawa和HEC-1A细胞24、48、72 h，采用MTT法检测细胞增殖率；Transwell检测细胞侵袭能力；流式细胞仪检测细胞凋亡水平；采用RT-PCR和Western blot分别检测VEGF、PI3K、AKT的mRNA和蛋白表达水平。 结果PNS可以显著抑制Ishikawa和HEC-1A细胞的增殖。与control组比较，PNS处理的Ishikawa和HEC-1A细胞侵袭显著下降（P＜0.05），细胞凋亡率明显升高（P＜0.05），VEGF和PI3K的表达水平下降（P＜0.05）。结论PNS能够抑制Ishikawa和HEC-1A细胞的增殖和侵袭，诱导细胞凋亡，其过程可能与VEGF/PI3K/AKT信号通路的抑制有关。

三七总皂苷；子宫内膜癌；血管内皮生长因子；PI3K/AKT信号通路

［Abstract］Objective To investigate the effects of Panaxnotoginsengsaponins（PNS）on the cell proliferation，invasion and apoptosis in endometrial cancercell line Ishikawa and HEC-1A and its underlying mechanism.Methods Ishikawa and HEC-1A cells were treated with different density of PNS for 24，48，72 h.The MTT method was used to detect the cell proliferation rate after Ishikawa and HEC-1A；cell invasion ability was detected by Transwell method；cell apoptosis was measured by flow cytometry；the mRNA and protein expression level of VEGF，PI3K and AKT were detected by RT-PCR and Western blot，respectively.Results PNS could significantly inhibited the cell proliferation of Ishikawa and HEC-1A cells.Compared with control group，the cell invasion ability of Ishikawa and HEC-1A apparently decreased（P＜0.05），and the cell apoptosis were elevated with PNS treatment（P＜0.05），the expression of VEGF and PI3K decreased with PNS treatment（P＜0.05）.Conclusion PNS inhibits the proliferation，invasion and induces apoptosis in Ishikawaand HEC-1A cells，which may be related to the inhibition of VEGF/PI3K/AKT signaling pathway.

［Key words］Panaxnotoginsengsaponins；Endometrial cancer；Vascular endothelial growth factor；PI3K/AKT

子宫内膜癌是三大妇科恶性肿瘤之一［1-2］。目前子宫内膜癌的治疗方法较多，以手术和放疗为主，辅助化学治疗或激素治疗，然而化学及激素治疗存在明显的复发症和毒副作用［3-4］。因此，寻找高效、安全、使用范围广的子宫内膜癌治疗新药是现今的研究热点。三七总皂苷（panaxnotoginsengsaponins，PNS）三七的主要有效成分，具有扩张血管、降低血脂和抗炎等药理作用［5-6］。已有研究表明，PNS对多种癌细胞的增值、凋亡和代谢等有影响［7-10］。但PNS在子宫内膜癌中的作用还未见报道。本研究拟以子宫内膜癌Ishikawa和HEC-1A细胞系为细胞模型，探讨PNS对子宫内膜癌细胞增殖、侵袭和凋亡的影响，并探讨其分子机制，为PNS治疗子宫内膜癌的临床应用提供理论依据。……