王新军　杨卓　杨如意　袁小威孙利坤牛晓阳

脑胶质瘤组织SNAT1蛋白表达及其意义☆

王新军*杨卓*杨如意*袁小威*孙利坤**牛晓阳**

目的探讨人钠耦合中性氨基酸转运蛋白1（human sodium coupled neutral amino acid transporter 1，SNAT1）在胶质瘤组织中的表达及其与临床病理参数，预后的关系。方法 用免疫组织化学法，蛋白质印迹法观察89例胶质瘤患者的胶质瘤组织和瘤周组织中的SNAT1的表达与分布，其中低级别胶质瘤55例（WHO Ⅰ-Ⅱ），高级别胶质瘤（WHOⅢ-Ⅳ）34例，采用两独立样本t检验分析蛋白质印迹中SNAT1的表达，采用单因素χ2检验分析SNAT1的表达与临床病理参数间的关系，采用Kaplan-Meier法观察SNAT1的不同表达对患者预后的影响，并建立Cox回归模型。结果 胶质瘤组织中SNAT1的表达明显高于瘤周组织（t=-9.803，P=0.001），高病理级别组织中SNAT1的表达明显高于低病理级别胶质瘤组织（t=-6.682，P=0.003）；SNAT1的表达与胶质瘤直径，病理级别有关（χ2=4.963，8.527，P＜0.05）；Cox回归模型显示肿瘤病理级别，SNAT1蛋白不同表达为影响胶质瘤患者预后的独立危险因素。结论 SNAT1蛋白的表达与胶质瘤病理级别及患者预后密切相关，有望成为判断胶质瘤生物学特征及评价患者预后的分子新靶点。

SNAT1胶质瘤 病理参数 预后

【Abstract】Objective To explore the expression of human sodium coupled neutral amino acid transporter 1 （SNAT1）in human glioma tissues and its relationship with clinical pathological parameters and prognosis.Methods Immu⁃nohistochemical and western blotting were used to detect SNAT1 expression in glioma tissue and tumor peripheral tissue from 89 cases of glioma patients including 55 cases of low grade gliomas（WHO I-II），and 34 cases of high grade gliomas （WHO grade III-IV）.χ2test and was used to analyze the relationship between expression and clinical pathological param⁃eters of SNAT1.Kaplan-Meier method was used to analyze the effect of different expression of SNAT1 on the prognosis of patients and to establish the Cox regression model.Results The expression of SNAT1 was significantly higher in gliomas than in tumor peripheral tissue（t=-9.803，P=0.001）.The expression of SNAT1 was significantly higher in high pathologi⁃cal grade tissues than in low grade of glioma tissues（t=-6.682，P=0.003）.SNAT1 expression was associated with tumor di⁃ameter and pathological grade（χ2=4.963，8.527，P＜0.05）；Cox regression model showed that the tumor pathological grade and different SNAT1 protein expression were independent risk factors for the prognosis of patients with glioma.Conclu⁃sions The expression of SNAT1 protein is closely associated with the pathological grade of gliomas and the prognosis of the patients，which may be a new target to judge the biological characteristics and to evaluate the prognosis of gliomas.

【Key Words】SNAT1 Gliomas Pathological parameters Prognosis

胶质瘤是神经系统最常见的肿瘤，其呈浸润性生长，患者的生存期短，死亡率高，预后差［1］。人钠耦合中性氨基酸转运蛋白1（human sodium cou⁃pled neutral amino acid transporter 1，SNAT1），又称SLC38A1，ATA1等，是一种Na+偶联的中性氨基酸转运蛋白，介导小分子物质如谷氨酸，丙氨酸等［2］，主要在胎盘，大脑和脊髓中表达［3］。近些年研究证明SNAT1在神经胶质瘤［4］，肝癌［5］，乳腺癌［6］中均异常高表达，本研究中我们通过免疫组织化学法，蛋白质印记法检测胶质瘤组织中SNAT1的表达与分布，并探讨SNAT1的表达与临床参数及患者预后的关系。……