何骁，邓莉莉，邹康（.郴州市第一人民医院中心医院骨科一区，湖南43000；.郴州市第一人民医院北院/儿童医院儿科，湖南43000）

菲立磁标记兔骨髓基质细胞自体移植坐骨神经的研究

何骁1，邓莉莉2，邹康1
（1.郴州市第一人民医院中心医院骨科一区，湖南423000；2.郴州市第一人民医院北院/儿童医院儿科，湖南423000）

目的在合适示踪剂帮助下，采用无创伤性影像学技术来在体识别、跟踪移植后骨髓基质细胞（BMSCs）的存活状态及与宿主神经组织整合情况，了解BMSCs移植周围神经后细胞如何迁移、如何凋亡和如何被吸收。方法BMSCs原代分离培养、诱导，菲立磁标记、鉴定及传代后注入坐骨神经损伤的兔模型中，并于细胞移植后第1、2、4、8、16周分别行磁共振成像（MRI）、透射电镜及免疫组织化学等。结果将菲立磁标记的干细胞移植后，采用MRI技术对兔坐骨神经进行扫描发现，以T1加权像（T1WI）和T2加权像（T2WI）序列检查可见移植菲立磁-多聚赖氨酸（FE-PLL）标记干细胞的坐骨神经区有明显低信号改变，T2WI扫描序列信号较T1WI明显。结论兔BMSCs源神经干细胞移植后，可在坐骨神经内存活、迁移、分化，T2WI序列成像可清晰显示菲立磁标记的BMSCs所致的低密度影像改变。利用MRI可以对移植后的标记细胞进行初步活体追踪。

移植，自体；坐骨神经；磁共振成像；菲立磁；骨髓基质细胞

【Abstract】Objective To adopt the non-invasive imaging technology in the help of suitable tracermaterial to in vivo recognize and track the survivalstatus of post-trans plantbone marrow stromal cells（BMSCs）and integration condition of hostnervous tissue，to understand themigration，apoptosis and absorption of cells after bonemarrow stromal cells autotransplant into peripheral nerve.M ethods BMSCs were primarily isolated，cultured，induced，Feridex labeled，identified and injected into the rabbitmodelof sciatic nerve injury after passage.Then MRI，transmission electron microscope and immunohis tochemicalex aminations were performed at1，2，4，8 and 16 weeks after cell transplantation.Results After Feridex labeled stem cell trans plantation，MRI scanning on rabbitsciatic nerve found that the obvious low signal change in the sciatic nerve area of FE-PLL labeled stem cells could be seen by the T1W1 and T2W1 sequence examinations，moreover the sequence signal of T2W1 scanningwas more significant than T1W1.Conclusion Theneuralstem celloriginated from rabbit BMSCs can survive，migrateand different iate after trans plantation.The T2W1 sequence imaging can clearly display the changeof low density imaging caused by Feridex labeled BMSCs.Using MRI can conduct thepreliminary in vivo trackingon labeled cellsafter transplantation.

【Keywords】Transplantation，autologous；Sciatic nerve；Magnetic resonance imaging；Feridex；Stromal cell

近年来干细胞疗法已成为治疗多种疾病的新策略，其目的是替代、修复及加强受损组织或器官的生物学功能。随着干细胞研究的深入，有关骨髓基质细胞（bonemarrow stormal cells，BMSCs）移植修复神经系统损伤的研究也越来越多。……